Chromatin remodeling and transcription of the TPK1 subunit of PKA during stress in Saccharomyces cerevisiae

Reca, Sol; Galello, Fiorella; Ojeda, Lucas; Pautasso, Constanza; Cañonero, Luciana; Moreno, Sílvia; Portela, Paula; Rossi, Silvia

doi:10.1016/j.bbagrm.2020.194599

Cited by 10 publications

(23 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously demonstrated that the TPK1 promoter, but not the other PKA subunit promoters, is activated upon heat shock and osmostress (Pautasso and Rossi, 2014;Reca et al, 2020). We also demonstrate that, during exponential growth, the BCY1 promoter has the lowest activity of the four PKA subunit promoters and does not respond to thermal stress (Pautasso and Rossi, 2014).…”

Section: Tpk1 Mrna Decay Kinetics During Heat Shockmentioning

confidence: 58%

“…Several levels of control acting simultaneously and in coordination allows the specificity of the cAMP-PKA signal transduction. Our previous results have demonstrated that the mechanisms involved in TPKs and BCY1 expression in response to stress or growth conditions are subunitspecific (Galello et al, 2017;Pautasso and Rossi, 2014;Reca et al, 2020).…”

Section: Discussionmentioning

confidence: 98%

“…TPK1 is the only PKA subunit that is transcriptionally upregulated during heat shock and saline stress (Pautasso and Rossi, 2014). Moreover, TPK1 promoter, but not TPK2 and TPK3, presents positioned nucleosomes that are evicted upon heat stress (Reca et al, 2020). We have previously described a complex network of specific expression regulators for each PKA subunit with a high-throughput screening (Pautasso et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Heat Stress Regulates the Expression ofTPK1Gene at Transcriptional and Post-Transcriptional Levels inSaccharomyces cerevisiae

Canononero

Pautasso

Galello

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

In Saccharomyces cerevisiae, cAMP regulates a number of different cellular processes, such as cell growth, metabolism, stress resistance and gene transcription. The intracellular target for this second messenger in yeast cells is the cAMP-dependent protein kinase (PKA). The way in which a broad specificity protein kinase mediates one right physiological response after cAMP increase indicates that specificity is highly regulated in the cAMP / PKA system. Here we address the mechanism through which cAMP-PKA signalling mediates its response to heat shock thermotolerance in Saccharomyces cerevisiae. Yeast PKA is a tetrameric holoenzyme composed of a regulatory (Bcy1) subunit dimer and two catalytic subunits (Tpk1, Tpk2 and Tpk3). PKA subunits are differentially expressed under certain stress conditions. In the present study we show that, although the mRNA levels of TPK1 are upregulated upon heat shock at 37℃, no change is detected in Tpk1 protein levels. The half-life of TPK1 mRNA increases and this mRNA condensates in cytoplasmic foci upon thermal stress. The resistance of TPK1 mRNA foci to cycloheximide-induced disassembly, together with the polysome profiling analysis suggest that TPK1 mRNA is impaired for entry into translation. TPK1 mRNA foci and TPK1 expression were also evaluated during thermotolerance. The crosstalk of cAMP-PKA pathway and cell wall integrity (CWI) signalling was also studied. Wsc3 sensor and other components of the CWI pathway are necessary for the upregulation of TPK1 mRNA upon heat shock conditions. The assembly in cytoplasmic foci upon thermal stress shows to be dependent of Wsc3. Finally, evidence of an increase in the abundance of Tpk1 in the PKA holoenzyme in response to heat shock is presented. The results indicate the existence of a mechanism that exclusively regulates Tpk1 subunit expression, which contributes to cAMP-PKA specificity and also suggest that a recurrent stress enhanced the fitness for the coming favorable conditions.

show abstract

Section: Tpk1 Mrna Decay Kinetics During Heat Shockmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Heat Stress Regulates the Expression ofTPK1Gene at Transcriptional and Post-Transcriptional Levels inSaccharomyces cerevisiae

Canononero

Pautasso

Galello

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…As high PKA activity is linked to lower stress tolerance, it seems plausible that regulation of PKA subunits at the transcriptional level might represent a mechanism to fine-tune PKA activity in response to stress. Some work has been carried out to study the regulation of TPK1-3 and BCY1 during heat shock and has identified that the TPK1 isoform is specifically upregulated, while TPK2 and TPK3 show no such increase in expression [ 101 , 102 , 103 ]. Curiously, a recent study of Tpk1 regulation during recurrent heat shock has shown that although TPK1 mRNA becomes more abundant, it is translationally inhibited and stored in mRNA granules until the stress is removed ( Figure 4 ) [ 103 ].…”

Section: Control Of the Heat Shock Response By The Camp/pka Pathwaymentioning

confidence: 99%

Yeast Protein Kinase A Isoforms: A Means of Encoding Specificity in the Response to Diverse Stress Conditions?

et al. 2022

View full text Add to dashboard Cite

Eukaryotic cells have developed a complex circuitry of signalling molecules which monitor changes in their intra- and extracellular environments. One of the most widely studied signalling pathways is the highly conserved cyclic AMP (cAMP)/protein kinase A (PKA) pathway, which is a major glucose sensing circuit in the yeast Saccharomyces cerevisiae. PKA activity regulates diverse targets in yeast, positively activating the processes that are associated with rapid cell growth (e.g., fermentative metabolism, ribosome biogenesis and cell division) and negatively regulating the processes that are associated with slow growth, such as respiratory growth, carbohydrate storage and entry into stationary phase. As in higher eukaryotes, yeast has evolved complexity at the level of the PKA catalytic subunit, and Saccharomyces cerevisiae expresses three isoforms, denoted Tpk1-3. Despite evidence for isoform differences in multiple biological processes, the molecular basis of PKA signalling specificity remains poorly defined, and many studies continue to assume redundancy with regards to PKA-mediated regulation. PKA has canonically been shown to play a key role in fine-tuning the cellular response to diverse stressors; however, recent studies have now begun to interrogate the requirement for individual PKA catalytic isoforms in coordinating distinct steps in stress response pathways. In this review, we discuss the known non-redundant functions of the Tpk catalytic subunits and the evolving picture of how these isoforms establish specificity in the response to different stress conditions.

show abstract

“…During stress conditions, heat shock and osmostress, the TPK1 promoter is the only one of the PKA subunit promoters that is upregulated. This promoter presents three Author's personal copy positioned nucleosomes that are evicted upon heat stress or osmostress, in correlation with promoter activation and upregulation of TPK1 mRNA levels (Pautasso and Rossi 2014;Reca et al 2020). It was demonstrated that the kinase Rim15 and the transcription factors Msn2/4, Gis1, and Sok2 are involved in TPK1 upregulation during heat shock (Pautasso and Rossi 2014).…”

Section: Transcriptional Regulation Of the Protein Kinase A Subunits ...mentioning

confidence: 99%

cAMP-PKA signal transduction specificity in Saccharomyces cerevisiae

Portela

Rossi

2020

Curr Genet

Self Cite

View full text Add to dashboard Cite

Your article is protected by copyright and all rights are held exclusively by Springer-Verlag GmbH Germany, part of Springer Nature. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

show abstract

Chromatin remodeling and transcription of the TPK1 subunit of PKA during stress in Saccharomyces cerevisiae

Cited by 10 publications

References 79 publications

Heat Stress Regulates the Expression ofTPK1Gene at Transcriptional and Post-Transcriptional Levels inSaccharomyces cerevisiae

Heat Stress Regulates the Expression ofTPK1Gene at Transcriptional and Post-Transcriptional Levels inSaccharomyces cerevisiae

Yeast Protein Kinase A Isoforms: A Means of Encoding Specificity in the Response to Diverse Stress Conditions?

cAMP-PKA signal transduction specificity in Saccharomyces cerevisiae

Contact Info

Product

Resources

About