Chromatin Regulators as Prognostic Biomarkers In Patients With Pheochromocytomas And Paragangliomas

Abstract

Chromatin regulators participated in tumorigenesis of various cancers while less research about its relationships with pheochromocytomas and paragangliomas. The aim of our study was to discover potential chromatin regulators as biomarkers for pathogenesis as well as prognosis of pheochromocytomas and paragangliomas. In this study, we explored expression patterns of pheochromocytomas and paragangliomas from the Gene Expression Omnibus database and 870 manually curated verified chromatin regulators. Then 185 differentially expressed chromatin regulators were verified which biological function were explored using Gene Ontology，Disease Ontology，Moreover, Kyoto Encyclopedia of Genes and Genomes pathways enrichment analysis. Besides protein-protein interaction network indicated 10 Hub genes. Their diagnostic performance as well as related drugs were valued by Receiver Operating Characteristics analysis, The Drug Signature Databases separately. Then we use TISIDB database to verify the relationship between 10 Hub genes and immune infiltration of pheochromocytomas and paragangliomas. Finally, 2 key genes were screened after Kaplan-Meier survival analysis and miRNA regulatory network was constructed based on Key genes by TargetScan microRNA 2017 in the Enrichr platform and TargetScanHuman (version8.0) platform. In conclusion, we identified 10 chromatin regulatorsespecially EZH2 and TAF7 which had high diagnostic performance and their functions were complicated in tumorigenesis of pheochromocytomas and paragangliomas. Likewise, the immune infiltration of pheochromocytomas and paragangliomas was intrinsically complex and these Hub genes mainly had negative correlation with immune infiltrating cells as well as immune infiltrating functions. Consequently, these chromatin regulators verified in our research could help to a further evaluation in pathogenesis and prognosis of pheochromocytomas and paragangliomas. Trial registration None