“…Additionally, recent super-resolution studies indicate that chromatin is also functionally organized in connected nano-scale compartments (Prakash et al, 2015;Szabo et al, 2018;Nir et al, 2018;Maiser ............................................................................................................................................................................. et al, 2020). Rapidly evolving methods of chromatin tracing (Boettiger et al, 2016;Wang et al, 2016;Beliveau et al, 2015;Nir et al, 2018;Bintu et al, 2018) and super-resolved imaging of the accessible genome (Xie et al, 2020) require sophisticated algorithms to analyze the topology of the generated paths (Goundaroulis et al, 2020). To understand the relationship between these complex structures and the underlying biological mechanism and functions of the genome (Bronshtein et al, 2015;Khanna et al, 2019;Leidescher et al, 2020 Preprint;Smeets et al, 2014), a more sophisticated and standardized analysis of SMLM data is urgently required.…”