Chromatin in the Cell Nucleus: Higher‐order Organisation

Abstract

Chromosome territories (CTs) constitute a major feature of nuclear architecture. Recent progress in three‐dimensional (3D) super‐resolution microscopy further supports the following functional model of chromatin organisation: CTs consist of interconnected assemblies of approximately 1 Mb chromatin domains (CDs). These domains are permeated by a 3D channel system, the so‐called interchromatin compartment (IC), which may serve as a preferential compartment for ribonucleic acid (RNA) transport. Wider parts of the IC are nearly deoxyribonucleic acid (DNA) free, expand between CTs and accommodate splicing speckles and nuclear bodies. The interior of CDs contains transcriptionally silent chromatin, whereas their periphery represents a zone of decondensed, transcriptionally competent chromatin. This perichromatin region borders on the network of IC channels and is the site of RNA transcription and DNA replication. During interphase large‐scale movements of CTs are typically absent, although exceptions may exist. In contrast, chromosome neighbourhood arrangements change profoundly during prometaphase resulting in variable CT neighbourhoods arrangements in cycling cells. Key Concepts: Each individual chromosome occupies a distinct region (territory) of the nuclear space. Chromosome territories (CTs) do not occupy fixed positions in the nucleus but show a polarised radial orientation: gene‐dense chromatin is typically located towards the nuclear interior and gene‐poor chromatin at the nuclear periphery. During interphase large‐scale movement of chromatin is not typically observed. Nuclear rotational movements are likely essential for chromatin reorganisation during post‐mitotic differentiation. In cycling cells profound repositioning of chromatin occurs during prometaphase, resulting in new CT neighbourhoods in subsequent daughter nuclei. Recently developed 3D super‐resolution light microscopy provides detailed insight into chromatin ultrastructure and increasing evidence for a highly compartmentalised functional organisation of CTs. We postulate that CTs are built up from interconnected approximately 1 Mb chromatin domains (CD). CDs are permeated by a network of channels, which constitute the interchromatin compartment (IC). The IC is connected to nuclear pores. It accommodates splicing speckles and nuclear bodies and provides a compartment for RNA transport. A zone of decondensed chromatin, called the perichromatin region (PR), is located at the periphery of CDs and lines the IC. It constitutes the site of transcription, splicing, DNA‐replication and possibly also DNA‐repair.

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus

“…Synchronization and cell cycle stage were confirmed by staining B35 cells with an antibody against γ-tubulin to assess centrosome duplication and positioning. At all stages of the cell cycle, RGS14 foci within the nucleus that were not localized to intranuclear channels were restricted to the periphery of or within regions of Hoechst-stained DNA, and were absent in adjacent DNA-poor interchromatin compartments (IC), which are thought to serve as a preferential compartment for RNA processing and transport [ 34 ] ( Fig 7 ). In B35 cell nuclei synchronized at G1, endogenous RGS14 staining was punctate along perichromatin regions between DNA-rich and DNA-poor subcompartments and was absent in highly compact chromatin areas typically associated with transcriptionally silenced genes [ 35 ] ( Fig 7A ).…”

Independent Theatre in Contemporary Europe : Structures – Aesthetics – Cultural Policy

“…209 Looking back on ten years of In-Presentable, one can easily recognise, however, the magnitude of this one-of-a-kind festival's contribution to the development of content and continuity.…”