Chromatin folding in human spermatozoa. I. Dynamics of chromatin remodelling in differentiating human spermatids

Arifulin, Eugene A.; Брагина, Е. Е.; Zamyatnina, V. A.; Volkova, E. G.; Sheval, Eugene V.; Golyshev, Sergey A.; Kintsurashvili, L. N.; Kiryanov, G. I.; Прусов, А. Н.; Polyakov, V. B.

doi:10.1134/s1062360411050031

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…The disturbance of chromatin condensation is a consequence of a reduced protamine content [14]. Hammoud et al [15] have recently found that defects in histone-to-protamine exchange lead to a random distribution of nucleosomal (histone-associated and potentially active) chromatin in infertile patients, in contrast to a programmed nucleosomal chromatin distribution in fertile men.…”

Section: Abnormal Chromatin Condensation In Spermatozoamentioning

confidence: 99%

Ultrastructure of Spermatozoa from Infertility Patients

Брагина¹,

Bocharova²

2018

Spermatozoa - Facts and Perspectives

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Standard examination of human semen currently remains a main test for examination of male fertility disorders. Although parameters of sperm quality in fertile men are generally higher than in sterile ones, there is a substantial overlap between the two populations, indicating that other important factors affect fertility, but are not assessed in conventional assay. Currently, tests determining the functional properties of sperm have been intensively developed. This review considers an electron microscopic examination of sperm, which assesses the structure and function of the sperm nuclear, penetration and motor apparatus. The detection of sperm chromatin structure can help to understand the causes of early embryonic malformation. Genetically caused and functional disorders of the structure and function of spermatozoa are discussed. Indications for electron microscopic examination of spermatozoa in fertility disorders are given.

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Section: Abnormal Chromatin Condensation In Spermatozoamentioning

confidence: 99%

Ultrastructure of Spermatozoa from Infertility Patients

Брагина¹,

Bocharova²

2018

Spermatozoa - Facts and Perspectives

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Biophysical Characterization of Human Protamine-1 as a Responsive CEST MR Contrast Agent

et al. 2014

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The protamines are a low-molecular-weight, arginine-rich family of nuclear proteins that protect chromosomal DNA in germ cells by packing it densely using electrostatic interactions. Human protamine-1 (hPRM1) has been developed as a magnetic resonance imaging (MRI) chemical exchange saturation transfer (CEST) reporter gene, based on a sequence that is approximately 50% arginine, which has a side chain with rapidly exchanging protons. In this study, we have synthesized hPRM1 and determined how its CEST MRI contrast varies as a function of pH, phosphorylation state, and upon noncovalent interaction with nucleic acids and heparin (as antagonist). CEST contrast was found to be highly sensitive to phosphorylation on serine residues, intra- and intermolecular disulfide bridge formation, and the binding of negatively charged nucleotides and heparin. In addition, the nucleotide binding constants (Keq) for the protamines were determined through plotting the molar concentration of heparin versus CEST contrast and compared between hPRM1 and salmon protamine. Taken together, these findings are important for explaining the CEST contrast of existing arginine-rich probes as well as serving as a guideline for designing new genetic or synthetic probes.

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Optimization of the sperm processing protocol for subsequent molecular cytogenetic studies

et al. 2021

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One of the causes of spontaneous pregnancy termination, infertility, and birth of children with development delay and malformations are chromosomal abnormalities (CA) as well as spontaneous aneuploidies in gametes of phenotypically normal parents. Often couples with reproductive problems, as well as spouses one of whom is a carrier of CA, turn to the programs of assisted reproductive technologies (ART) for preimplantation evaluation of the zygote chromosomal status. As part of ART programs, parental gametes are examined to assess the level of spontaneous aneuploidy. As a rule, the most accessible material for analysis is the ejaculate. Fluorescent in situ hybridization (FISH) is used to examine male gametes obtained from the ejaculate. However, this FISH-analysis has a number of limitations and difficulties because of the peculiarities of the sperm head structure, namely the supercondensed state of chromosome chromatin. In order to optimize the FISH protocol, five different protocols were used for pre-hybridization processing of ejaculate samples obtained from nine phenotypically normal men. A comparative analysis of hybridization efficiency showed that the protocol using tris(2-carboxyethyl)phosphine hydrochloride (TCEP) as a decondensation agent was the most effective for subsequent molecular cytogenetic studies. The developed hybrid protocol combining proteolytic pretreatment, TCEP and thermal decondensation can be used when other protocols for pre-hybridization treatment of ejaculate preparations are not effective.

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Chromatin folding in human spermatozoa. I. Dynamics of chromatin remodelling in differentiating human spermatids

Cited by 3 publications

References 23 publications

Ultrastructure of Spermatozoa from Infertility Patients

Ultrastructure of Spermatozoa from Infertility Patients

Biophysical Characterization of Human Protamine-1 as a Responsive CEST MR Contrast Agent

Optimization of the sperm processing protocol for subsequent molecular cytogenetic studies

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