Chromatin endogenous cleavage provides a global view of RNA polymerase II transcription kinetics
Jake VanBelzen,
Bennet Sakelaris,
Donna Garvey Brickner
et al.
Abstract:Chromatin immunoprecipitation (ChIP-seq) is the most common approach to observe global binding of proteins to DNA
in vivo
. The occupancy of transcription factors (TFs) from ChIP-seq agrees well with an alternative method, chromatin endogenous cleavage (ChEC-seq2). However, ChIP-seq and ChEC-seq2 reveal strikingly diUerent patterns of enrichment of yeast RNA polymerase II. We hypothesized that this reflects distinct populations of RNAPII, some of which are captured by ChIP-seq and some of which are captured b… Show more
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