Chromatin and the cell cycle meet in Madrid

Domı́nguez, Marı́a; Berger, Frédéric

doi:10.1242/dev.000844

Cited by 4 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These indicate that 2-d timing could be correlated with one to two cell division cycles. The next challenge is to reveal how AG is involved in the dynamics of epigenetic marks and to understand a possible link between chromatin modification and cell cycle progression (Dominguez and Berger 2008).…”

Section: Ag Directly Induces Knu With a 2-d Time Lag In Flower Develomentioning

confidence: 99%

A timing mechanism for stem cell maintenance and differentiation in the Arabidopsis floral meristem

Sun¹,

Xu²,

Ng³

et al. 2009

Genes Dev.

262

299

View full text Add to dashboard Cite

Developmental regulation of the floral meristem ensures that plants of the same species have similarly sized flowers with a fixed number of floral organs. The maintenance of stem cells in the floral meristem is terminated after the production of a fixed number of floral organ primordia. Precise repression of the Arabidopsis thaliana homeobox gene WUSCHEL (WUS) by the floral homeotic protein AGAMOUS (AG) plays a major part in this process. Here we show that KNUCKLES (KNU) mediates the repression of WUS in floral meristem determinacy control. AG directly induces the transcription of KNU, which encodes a C2H2-type zinc finger protein with a conserved transcriptional repression motif. In turn, KNU represses WUS transcription to abolish stem cell activity. We also show that the timing of KNU induction is key in balancing proliferation and differentiation in flower development. Delayed KNU expression results in an indeterminate meristem, whereas ectopic KNU expression prematurely terminates the floral meristem. Furthermore, KNU induction by AG is preceded by changes in repressive histone modification at the KNU locus, which occurs in an AG-dependent manner. This study provides a mechanistic link between transcriptional feedback and epigenetic regulation in plant stem cell proliferation.[Keywords: Arabidopsis thaliana; flower development; homeotic control; MADS protein; AGAMOUS; WUSCHEL; stem cell] Supplemental material is available at http://www.genesdev.org.

show abstract

Section: Ag Directly Induces Knu With a 2-d Time Lag In Flower Develomentioning

confidence: 99%

A timing mechanism for stem cell maintenance and differentiation in the Arabidopsis floral meristem

Sun¹,

Xu²,

Ng³

et al. 2009

Genes Dev.

262

299

View full text Add to dashboard Cite

show abstract

“…Therefore, derangement of steady shear stress leads to cell proliferation, which may cause atherosclerosis through disruption of EC stability (Akimoto et al, 2000). The stages of the cell cycle are regulated by epigenetic modification (Dominguez and Berger, 2008), but the mechanism of assembly and dynamics of histone modification in cell cycle regulation are not known.…”

Section: Introductionmentioning

confidence: 99%

Maintenance of HDACs and H3K9me3 Prevents Arterial Flow-Induced Venous Endothelial Damage

Wang

Chang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The transition of flow microenvironments from veins to arteries in vein graft surgery induces “peel-off” of venous endothelial cells (vECs) and results in restenosis. Recently, arterial laminar shear stress (ALS) and oscillatory shear stress (OS) have been shown to affect the cell cycle and inflammation through epigenetic controls such as histone deacetylation by histone deacetylases (HDACs) and trimethylation on lysine 9 of histone 3 (H3K9me3) in arterial ECs. However, the roles of H3K9me3 and HDAC in vEC damage under ALS are not known. We hypothesized that the different responses of HDACs and H3K9me3 might cause vEC damage under the transition of venous flow to arterial flow. We found that arterial ECs showed high expression of H3K9me3 protein and were retained in the G0 phase of the cell cycle after being subjected to ALS. vECs became round under ALS with a decrease in the expression of H3K9me3, HDAC3, and HDAC5, and an increase in the expression of vascular cell adhesion molecule 1 (VCAM-1). Inhibition of HDACs activity by a specific inhibitor, phenylbutyrate, in arterial ECs caused similar ALS-induced inflammation and cell loss as observed in vECs. Activation of HDACs and H3K9me3 by ITSA-1, an HDAC activator, could prevent ALS-induced peel-off and reduced VCAM-1 expression in vECs. Moreover, shear stress modulates EC morphology by the regulation of focal adhesion kinase (FAK) expression. ITSA-1 or EGF could increase phosphorylated (p)-FAK expression in vECs under ALS. We found that perturbation of the activity of p-FAK and increase in p-FAK expression restored ALS-induced H3K9me3 expression in vECs. Hence, the abnormal mechanoresponses of H3K9me3 and HDAC in vECs after being subjected to ALS could be reversed by ITSA-1 or EGF treatment: this offers a strategy to prevent vein graft failure.

show abstract

Epigenetic reprogramming of H3K27me3 and DNA methylation during leaf-to-callus transition in peach

Zheng

Liu

An-qi

et al. 2022

Horticulture Research

View full text Add to dashboard Cite

Plant tissues are capable of developing unorganized cells masses termed callus in response to the appropriate combination of auxin and cytokinin. Revealing the potential epigenetic mechanisms involved in callus development can improve our understanding of the regeneration process of plant cells, which will be benefit for overcoming regeneration recalcitrance in peach. In this study, we report on single-base resolution mapping of DNA methylation and reprogramming of the trimethylation of histone H3 at lysine 27 (H3K27me3) pattern at a genome-wide level during the leaf-to-callus transition in peach. Overall, the predominant mCG and mCHH at the genome-wide level and the predominant mCG in genic regions were observed in peach. H3K27me3 deposition was mainly detected in gene-body and the TSS site, and GAGA repetitive sequences were prone to recruit H3K27me3 modification. H3K27me3 methylation was negatively correlated with gene expression. In vitro culture of leaf explant was accompanied with DNA hypomethylation and H3K27me3 demethylation, which could activate auxin- and cytokinin-related regulators to induce callus development. DNA methylation inhibitor 5-azacytidine could significantly increase callus development, while H3K27me3 demethylase inhibitor GSK-J4 dramatically reduced callus development. These results demonstrate the roles of DNA methylation and H3K27me3 modification in mediating chromatin status during callus development. Our study provides new insights into epigenetic mechanisms through which differentiated cells acquire proliferative competence to induce callus development in plants.

show abstract

Chromatin and the cell cycle meet in Madrid

Cited by 4 publications

References 48 publications

A timing mechanism for stem cell maintenance and differentiation in the Arabidopsis floral meristem

A timing mechanism for stem cell maintenance and differentiation in the Arabidopsis floral meristem

Maintenance of HDACs and H3K9me3 Prevents Arterial Flow-Induced Venous Endothelial Damage

Epigenetic reprogramming of H3K27me3 and DNA methylation during leaf-to-callus transition in peach

Contact Info

Product

Resources

About