2022
DOI: 10.1186/s13072-021-00433-4
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Chromatin alterations during the epididymal maturation of mouse sperm refine the paternally inherited epigenome

Abstract: Background Paternal lifestyle choices and male exposure history have a critical influence on the health and fitness of the next generation. Accordingly, defining the processes of germline programming is essential to resolving how the epigenetic memory of paternal experiences transmits to their offspring. Established dogma holds that all facets of chromatin organization and histone posttranslational modification are complete before sperm exits the testes. However, recent clinical and animal stud… Show more

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Cited by 12 publications
(7 citation statements)
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“…The increasing number of data indicates changes of epigenetic markers of sperm DNA also during the maturation in epididymis (and its particular parts), and thus puts attention also on the epigenetic role of this organ, leading to the need of revision of the statement that the histone PTMs are completed before the release of the sperm from the male gonad [ 21 , 217 , 223 ]. So, in the context of male reproduction there is a strong need to deep evaluation of the epididymal processes in the epigenetic manner, particularly in the light of the known rich epididymal microenvironment and its already described influence both: on sperm maturation (motility, fertilization capability), as well as on the rate of sperm DNA damage [ 21 , 202 , 217 220 , 223 ]. Thus, the link between proper epimarks and sperm chromatin integrity perhaps will possess part of its principles also from the epididymal side.…”
Section: Discussionmentioning
confidence: 99%
“…The increasing number of data indicates changes of epigenetic markers of sperm DNA also during the maturation in epididymis (and its particular parts), and thus puts attention also on the epigenetic role of this organ, leading to the need of revision of the statement that the histone PTMs are completed before the release of the sperm from the male gonad [ 21 , 217 , 223 ]. So, in the context of male reproduction there is a strong need to deep evaluation of the epididymal processes in the epigenetic manner, particularly in the light of the known rich epididymal microenvironment and its already described influence both: on sperm maturation (motility, fertilization capability), as well as on the rate of sperm DNA damage [ 21 , 202 , 217 220 , 223 ]. Thus, the link between proper epimarks and sperm chromatin integrity perhaps will possess part of its principles also from the epididymal side.…”
Section: Discussionmentioning
confidence: 99%
“…We acid extracted histones using methods previously described 73 . Briefly, we resuspended frozen sperm pellets in Nuclei Isolation Buffer-250 with 0.3% NP-40 (15 mM Tris–HCl (pH 7.5), 60 mM KCl, 15 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 250 mM Sucrose, 1 mM DTT, 10 mM sodium butyrate, and 1:100 Halt protease inhibitor (Cat# PI78437, Thermo Fisher Scientific, Pittsburgh, PA, USA)) and rotated them at 4 °C for 30 min.…”
Section: Methodsmentioning
confidence: 99%
“…While predominantly located at transcriptionally active promoters in round spermatids [9] , H4ac levels are globally upregulated upon spermatid elongation 10 , 11 , 12 . Subsequently, H4ac dramatically decreases concomitantly with histone eviction, resulting in only ~2–5% of H4 peptides acetylated at residues K5, K8, or K12 in mature sperm [13] . In vitro, protamines displace hyperacetylated nucleosomes more efficiently than unmodified ones [14] .…”
Section: Nucleosome Eviction and Retention During The Histone-to-prot...mentioning
confidence: 99%