Chromatidaustausch und Heterochromatinver�nderungen menschlicher Chromosomen nach BUdR-Markierung

Kim, My. A.

doi:10.1007/bf00281425

Cited by 62 publications

(9 citation statements)

References 28 publications

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“…2), there is a relatively good agreement between our results and those reported by KIM (1974) and LATT (1974a) at BrdU concentrations between 50-100 pM. The frequency reported by DUTRILLAUX et al (1974), 27 SCE/cell at BrdU concentration of 600 pM, is about twice as high as that reported by LATT (1974a), KIM (1974) and GALLOWAY and EVANS (1975) for BrdU concentrations between 20 and 160 pM (see Fig. 2).…”

Section: Discussionsupporting

confidence: 91%

“…Similar results were obtained by DUTRILLAUX et al (1974) and LATT (1974b). There is one exception to this general rule, and that is centromeric exchanges (CME), which seem to occur in an unexpectedly high frequency (KIM 1974;TICE et al 1975). Other authors have either avoided the inclusion of C M E s in the calculation of SCE-frequency (CHAGANTI et al 1974) referring to the difficulty to distinguish between SCE and chromosome twists, or neglected to present the distinction between CME and SCE.…”

Section: Discussionsupporting

confidence: 85%

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Bromodeoxyuridine-induced sister chromatid exchanges in human lymphocytes

et al. 2009

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Human lymphocytes were cultivated for 75 hours in the presence of various concentrations of bromodeoxyuridine (BrdU). The cells were stained with the tluorochrome Hoechst 33258 plus Giemsa. Cells in the first, second and third division could be distinguished, as well as sister chromatid exchanges (SCE) and centromeric exchanges (CME). BrdU-concentrations higher than 100 pM decreased the relative number of cells in the third division and increased the number of cells in the first division in vitro, indicating that higher BrdU-concentrations considerably inhibit cell propagation. The frequency of SCE and CME increased significantly for each stepwise rise in the BrdU concentration between 20-500 pM, suggesting that the vast majority of SCE and CME are induced by BrdU. The average frequency of SCE per cell in 100 pM of BrdU ranged between 18-28 for 8 control subjects with a group mean of 22.2k3.4. BrdU-induced SCE were found to be equally distributed between chromosomes in relation to their lengths while the frequency of CME was independent of chromosome length. High concentrations of BrdU (200--500 pM) significantly increased the number of chromosome aberrations in the cells of all of four subjects investigated. The aberrations were mainly chromosome breaks. Bo Lambert, Department of Clinical Genetics, Kurolinska Hospital, S-104 01 Stockholm 60, SwedenThe recently developed technique for the detection of sister chromatid exchanges (SCE) as originally described by LATT (1973) has attracted immediate interest as a potential screening method for chromosomal effects of mutagenic and carcinogenic agents (SAVAGE 1975). It has been shown to be more sensitive than the conventional analysis of chromosome aberrations with regard to some mutagens and carcinogens tested (LATT 1974a;PERRY and EVANS 1975) and it appears to be less laborious. The technique is based on the property of sister chromatids to quench fluorescence differentially after staining with the fluorochrome Hoechst 33258, provided that the cells have passed through two subsequent S-phases in the presence of bromodeoxyuridine (BrdU). Later modifications of the technique permit permanent preparations to be made, in which sister chromatids are, stained differentially by Giemsa ( WOLFF and PERRY 1974;KORENBERG and FREEDLENDER 1974; GOTO eta]. 1975).The biological significance of SCE in somatic cells is not known and it remains unclear whether SCE occurs or not in vivo. The demonstration of SCE in vitro depends on the usage of BrdU which itself may give rise to SCE (WOLFF and PERRY 1974;KATO 1974;LATT 1974a). The purpose of the present investigation was to study the effect of BrdU on cell turnover and the frequency of SCE and chromosome aberrations in peripheral lymphocytes from a number of healthy subjects, as a basis for future in vivo and in vitro experiments. Our results clearly demonstrate a dependence of SCE frequency on increasing concentrations of BrdU. At higher concentrations of BrdU there is an increased frequency of chromosome breaks and cell turnover i...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

Bromodeoxyuridine-induced sister chromatid exchanges in human lymphocytes

et al. 2009

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“…The mean exchange frequency per cell in man varies rather widely from one individual to another. While several authors found a mean of 14 exchanges per cell (Latt 1973, Kim 1974, Chaganti et al 1974, Galloway and Evans 1975, others find 4 or 5 (Beek and Obe 1975, Sperling et al 1976), 8 (Sperling et al 1975, 12 (Latt 1974) or 27 (Dutrillaux et al 1974). The mean frequency of 12.2 (1.5-31) in cattle is similar to that often found in man.…”

Section: Discussionmentioning

confidence: 99%

Sister chromatid exchanges (S.C.E.) in normal and abnormal bovine (Bos taurus L.) karyotypes.

Popescu¹

1978

CYTOLOGIA

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“…Chromosome banding with "33258 Hoechst" under various conditions were reported by several authors (Hilwig and Gropp, 1972;Natrajan et aL, 1973;Seth and Gropp, 1973;Latt, 1973;Kim, 1974;Raposa and Natarajan, 1974;Holmquist, 1975;Jalal et al, 1975). Natarajan et al (1973) described that with the manipulation of the staining intensity with this dye, only bands corresponding to C-bands or those corresponding to both C-and Q-bands were obtained.…”

Section: Discussionmentioning

confidence: 92%

Staining properties of a benzimidazol derivative “33258 hoechst” and a simplified staining method for chromosome banding

et al. 1977

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SummaryTo obtain good chromosome banding with "33258 Hoechst," various conditions were examined. For fluorescence microscopy, an UV filter for excitaion and a BV filter for emission were selected from spectrofluorometric examination. "33258 Hoechst" was dissolved in distilled water and good chromosome banding was obtained when slides were stained at room temperature, for 10 to 20 minutes at the concentration between 4 ~g/ml to 0.2/~g/ml of this dye. The aging of slides before and after staining was shown to be another important factor. Chromosome banding revealed with "33258 Hoechst" represents chromosome banding corresponding to both Q-and C-bandings in a very simple way. The mechanism of chromosome banding with "33258 Hoechst" is briefly discussed.

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Chromatidaustausch und Heterochromatinver�nderungen menschlicher Chromosomen nach BUdR-Markierung

Cited by 62 publications

References 28 publications

Bromodeoxyuridine-induced sister chromatid exchanges in human lymphocytes

Bromodeoxyuridine-induced sister chromatid exchanges in human lymphocytes

Sister chromatid exchanges (S.C.E.) in normal and abnormal bovine (Bos taurus L.) karyotypes.

Staining properties of a benzimidazol derivative “33258 hoechst” and a simplified staining method for chromosome banding

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