In this article, an SPR sensor based on 12-fold quasi-periodic PCF composed of Ge-doped silica is introduced for analyte detection. The outer surface of the cladding is covered by a 40 nm gold layer serving as the plasmonic layer for the proposed sensor. The birefringence induced due to the inherent characteristic of the 12-fold pattern in the cross-section provides discrepancy for the operation of the structure regarding x- and y-polarizations. The wavelength sensitivity, amplitude sensitivity, and resolution of 12150 nm.RIU−1, 678 RIU−1, and 8.23 × 10–6 RIU, respectively are obtained for the proposed sensor. The FEM method has been implemented to investigate the performance of the proposed sensor for biosensing application in a detection range of 1.33–1.41.