ChromagarTM Col-Apse for Detection of Colistin Resistance in Clinical Isolates of Multidrug Resistant Gram Negative Bacilli

Ali, Saad Emhmed; Hussain, Wajid; Ahmed, Farooq; Afzal, Raja Kamran; Mirza, Irfan Ali; Khurshid, Umer

doi:10.29271/jcpsp.2022.02.177

Cited by 1 publication

(2 citation statements)

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“…In this study, CHROMAgar Col- APSE produced the highest rate of MEs and a high VME second only to that of the in-house Rapid NP test (Tables 1-2), particularly for Klebsiella spp. Although the specificity recorded in this study and that of Osei Sekyere et al (at 66,67%) agree, two other studies recorded a significantly higher specificity of ≥ 97% [11,22,23]. These discrepancies could be attributed to the different bacterial inoculum concentrations used.…”

Section: Discussioncontrasting

confidence: 52%

“…These discrepancies could be attributed to the different bacterial inoculum concentrations used. Ali et al demonstrated that when a 0.5 McFarland standard inoculum was used, colistin- susceptible isolates were able to grow on the media [23], whereas the same isolates were inhibited when the inoculum was diluted to a density of 1×105 CFU/mL. Therefore, the high rate of MEs in this present study could be due to the inoculum used being a 0.5 McFarland standard (1.5×108 CFU).…”

Section: Discussionmentioning

confidence: 78%

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Evaluation of Six Commercial and Non-Commercial Colistin Resistance Diagnostics

Leshaba,

Mmatli,

Skosana

et al. 2024

Preprint

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Background. Resistance to colistin, a last-reserve antibiotic used for treating drug-resistant infections, is increasing globally. This study evaluated six diagnostic tests designed to detect colistin-resistant pathogens. Methods. PCR and broth microdilution assays (BMD) were used to respectively characterize the molecular mechanisms and phenotypic colistin resistance of 142 Gram-negative bacterial isolates and controls. The sensitivity, specificity, positive- and negative-predictive values, major (ME) and very major errors (VME), categorical and essential agreements (EA) of ComASP Colistin, CHROMagar COL-APSE, Rapid NP Test, Sensititre, MicroScan, and Vitek 2 were determined with these isolates; the BMD was used as gold standard. Results. The Vitek 2, Sensititre, and ComASP tests were more efficient, albeit with concerning ME and VMEs and low EAs. Sensititre was 100% specific with 0% ME and 3.61% VME; Vitek 2 had the least VME (1.25% and 0%) and a low EA (57.50%). ComASP had an EA of 75.35%. MicroScan was highly sensitive (96.55%) but less specific (87.50%), with very below-accetable EAs (48.11%). The CHROMAgar COL-APSE efficiently identified the species with their unique colours but was the least specific (67.80%), with the highest ME (32.20%) and high VME (7.23%). The Rapid NP test had the highest VME (7.84%), producing results within 4 hours with 92.16% sensitivity and 96.08% specificity. Conclusion: Vitek 2, MicroScan, ComASP colistin, and Sensititre are good for determining colistin resistance; the latter two tests are recommendable for low-resourced laboratories. The in-house Rapid NP test has short turnaround time with high efficiency for initial resistance screening.

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Section: Discussioncontrasting

confidence: 52%

Section: Discussionmentioning

confidence: 78%