CHP2 gene expression and quantitation in Egyptian patients with acute leukemia

Abstract

AimCHP2 (calcineurin B homologous protein 2) is identified as a tumor-associated antigen highly expressed in different malignancies. It plays a critical role in cancer cell development, proliferation, motility and survival. It is suggested that the human tumor related gene CHP2 expression in leukemia primary cells and leukemia cell lines significantly increase, which may play an important role in growth process of leukemia cells.MethodsIn this study, the expression of CHP2 gene was analyzed in 10 normal healthy controls and 40 patients with de novo acute leukemia (20 AML and 20 ALL). CHP2 expression was analyzed using a real-time quantitative reverse-transcriptase polymerase chain reaction (RTQ-PCR) to investigate a possible relation, association or correlation with the clinical features of AL (acute leukemia) at diagnosis, such as age, gender, lineage, HB, TLC, platelet count, BM blast cell infiltration and risk group.ResultsCHP2 was highly expressed in 13/40 AL studied patients (7/20 AML and 6/20 ALL) with mean expression level of 2.7 while it was not expressed in any of the controls.ConclusionsMany studies suggest that CHP2 expression is a novel prognostic marker in AL and thus needs to be incorporated into the patient stratification and treatment protocols. In addition, a quarter of AL patients fail therapy and novel treatments that are focused on undermining specifically the leukemic process are needed urgently.

CHP2 Promotes Cell Proliferation in Breast Cancer via Suppression of FOXO3a

“…Univariate and multivariate analyses indicated that CHP2 overexpression might function as an independent prognostic factor for breast cancer patients. Previous reports have provided evidence that CHP2 is involved in cell growth in acute leukemia and ovarian cancer (20,22). Consistent with these reports, we found that CHP2 was strongly expressed in highly proliferative lesions of human breast cancer, as indicated by a significant correlation between CHP2 and Ki67 expressions (P < 0.01).…”

“…In accordance with this, CHP2-transfected cells showed an increased nuclear presence of NFATc3 and enhanced NFAT activity (19). In addition, Hammam and colleagues found that the expression of CHP2 in leukemia primary cells was significantly increased, which might play an important role in leukemia cell growth (20). These results highlighted a potential role of CHP2 in tumorigenesis and revealed a novel function of CHP2 as an activator of the calcineurin/NFAT signaling pathway (21).…”

Identification of novel biomarkers affecting the metastasis of colorectal cancer through bioinformatics analysis and validation through qRT-PCR

“…calcineurin homologous protein isoform 2 (CHP2) is expressed in normal intestinal epithelia and also epithelium-like cell line [25], and it might act as a potential role in transmembrane Nat/Ht exchange which can protect cells from serum deprivation induced death [25,26]. The expression of CHP2 has been reported to be significantly increased in human ovarian carcinoma cells [27] and leukemia primary cells [28] and breast cancer cells [29]. Mechanistically, overexpression of CHP2 activated AKT signaling and suppressed the transactivation of the forkhead box O3 (FOXO3/FOXO3a) transcription factor in breast cancer [29].…”

Insights Into the Albinism Mechanism for Two Distinct Color Morphs of Northern Snakehead, Channa argus Through Histological and Transcriptome Analyses

“…In order to verify the RNA-seq results, ten pigment-related genes (Casp et al, 2002;Bhoumik et al, 2007;Mirmohammadsadegh et al, 2010;Liu et al, 2013;Hammam et al, 2014;Wang et al, 2017;Yang et al, 2018;Li et al, 2020) were selected to quantify their mRNA expression levels in the skin of two distinct color morphs of C. argus by using qRT-PCR (Figure 11). The expressions of all the selected genes of BT were extremely higher than that of AT, consistent with the results obtained through transcriptome analysis.…”

“…Meanwhile, the MITF is an upstream TF that can directly regulate the TYR gene (Bharti et al, 2012;Raviv et al, 2014), and some genes such as SOX10, MAPK, CATs act directly or indirectly on the MITF gene to regulate the development, growth and differentiation melanocytes (Hou et al, 2000). In addition, the MLPH, CHP2, and EpoR play roles in the formation, transportation, development and other processes of pigment cells and melanoma (Bhoumik et al, 2007;Liu et al, 2013;Hammam et al, 2014;Li et al, 2020). Previous studies showed that the differential expression of albino-related genes regulated the development and differentiation of body color, and over 95 and 38 body color development related genes were found in zebrafish and Oryziaslatipes (Hidehito et al, 1994;Dooley et al, 2013).…”