Chorionic gonadotrophin in the mouse from implantation to term

Wide, Leif; Wide, Mariann

doi:10.1530/jrf.0.0570005

Cited by 26 publications

(16 citation statements)

References 10 publications

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“…This has also been observed in extracts of placentae from the rat, hamster and mouse [Wide and Hobson, 1978;Wide and Wide, 1979]. A molecular form of p subunit more retarded than that of the a subunit has also been reported in extracts of human pregnancy urine and placentae after chroma tography on Sephadex G-100 [Good et al, 1977].…”

Section: Discussionmentioning

confidence: 69%

The Similarity of Chorionic Gonadotrophin and Its Subunits in Term Placentae from Man, Apes, Old and New World Monkeys and a Prosimian

Hobson¹,

Wide²

1981

IJFP

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Chorionic gonadotrophin (CG) was estimated, by bioassay and radioimmunoassay (RIA), in placental extracts from 11 ape and monkey species. There was a significant correlation between the results of the two assay systems (r = 0.903, p < 0.001). The concentration of CG in most primate term placentae was the same as that in the human placenta at term. Extracts from all placentae cross-reacted with antiserum to ovine LH-β subunit, and those of the chimpanzee and gorilla also had a significant cross-reaction with an antiserum to the carboxyl terminal peptide of the HCG-β subunit. Primate placentae chromatographed on Sephadex G-200 had components active in the RIA systems for HCG, HCG-α HCG-β sub-units. In general, the elution profiles of all ape and monkey placental extracts resemble those made from human term placentae and of purified HCG and its subunits. The shape of the elution patterns from human and non-human material suggests that there was more than one molecular form of CG-α subunit activity. A second, more retarded molecular form having β subunit activity was found in extracts made from human, gorilla, gibbon and rhesus monkey placentae. The similarity between the structure of ape and monkey placental CG with HCG and its subunits implies a function similar to that of HCG in late pregnancy.

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Section: Discussionmentioning

confidence: 69%

The Similarity of Chorionic Gonadotrophin and Its Subunits in Term Placentae from Man, Apes, Old and New World Monkeys and a Prosimian

Hobson¹,

Wide²

1981

IJFP

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“…However, Astwood & Greep (1938) provided evidence for an extra-pituitary source of luteotrophin in the pregnant rat which was later referred to as "chorionic gonadotrophin" by Astwood (1953). More recently, extracts or expiants from placentae of hamster, mouse, rat and sheep have behaved in a number of biological and non-biological assays in a manner which suggests a similarity between ovine, rodent and human CG (Linkie & Niswender, 1973;Okker-Reitsma, 1978;Haour, Tell & Sanchez, 1976; Wide & Hobson, 1978;Wide & Wide, 1979;Lacroix & Martal, 1979;Okker-Reitsma & Wilson, 1980; Wide, Hobson & Wide, 1980).…”

Section: Introductionmentioning

confidence: 99%

Gonadotrophic activity of the guinea-pig placenta during pregnancy

Humphreys¹,

Hobson²,

Wide³

1982

Reproduction

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Summary. A gonadotrophic substance was extracted from placentae and subplacentae of guinea-pigs. Biological and immunological assays of these tissues obtained on Days 10\p=n-\63of pregnancy showed the highest mean concentration of the gonadotrophin on Day 18. Extracts chromatographed on Sephadex G200 had components active in the radioimmunoassay system for hCG, hCG-alpha and hCG-beta subunits. The elution profiles of these extracts were similar to those obtained with human term placentae and purified hCG and its subunits. The guinea-pig placental extracts also cross-reacted with an antiserum to the beta subunit of ovine LH, as did hCG. The biological activity of placental and subplacental extracts and of the hCG standard was neutralized by an anti-hCG serum. There is a strong similarity between hCG and the substance extracted from guinea-pig placentae which may therefore have a luteotrophic function similar to that of hCG.

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“…The contribution of uterine LHCGR signalling to the infertility of LuRKO mice was demonstrated by one group (Lin et al 2005); however, conflicting results were published by Pakarainen et al (2005), who were unable to show that orthotopic transplantation of wild-type ovaries into an LuRKO mouse resulted in impaired implantation. Nevertheless, although LHCGR has been identified previously in murine endometrium (Mukherjee et al 1994) and an embryonic LH/ hCG-like signal has been detected throughout the pregnancy in the conceptus, placenta (Wide and Wide 1979) and plasma (Gidley-Baird 1977), these findings were never confirmed or have been the subject of controversy in the field (Roberts et al 1996). In addition, to date the endometrial LHCGR ligand has never been identified in mouse blastocysts.…”

Section: Discussionmentioning

confidence: 95%

Evidence for cross-talk between the LH receptor and LH during implantation in mice

Gridelet

Tsampalas

Berndt

et al. 2013

Reprod. Fertil. Dev.

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Abstract. The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation.

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Chorionic gonadotrophin in the mouse from implantation to term

Cited by 26 publications

References 10 publications

The Similarity of Chorionic Gonadotrophin and Its Subunits in Term Placentae from Man, Apes, Old and New World Monkeys and a Prosimian

The Similarity of Chorionic Gonadotrophin and Its Subunits in Term Placentae from Man, Apes, Old and New World Monkeys and a Prosimian

Gonadotrophic activity of the guinea-pig placenta during pregnancy

Evidence for cross-talk between the LH receptor and LH during implantation in mice

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