Chorioallantoic membrane tumor models highlight the effects of cisplatin compounds in oral carcinoma treatment

Sarogni, Patrizia; Mapanao, Ana Katrina; Gonnelli, Alessandra; Ermini, Maria Laura; Marchetti, Sabrina; Kusmic, Claudia; Paiar, Fabiola; Voliani, Valerio

doi:10.1016/j.isci.2022.103980

Cited by 19 publications

(52 citation statements)

References 73 publications

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“…Eggs were treated with NAs-cisPt-Tf2 received 0.7 mM cisplatin (~4 μg Pt per egg). This concentration was equivalent to the administered amount of cisplatin and NAs-cisPt in the previous investigations [26].…”

Section: Cam Tumor Treatment and Monitoringmentioning

confidence: 97%

“…Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene and served as loading control. The sequences of forward and reverse primers are listed in the previous study [26]. The amplification curves were visualized using SYBR Green Analysis on Applied Biosystems Instrument (7300).…”

Section: Hematoxylin and Eosin (Hande) Stainingmentioning

confidence: 99%

“…A standardized method tailored for SCC-25 neoplasms was implemented to have a consistent biological model and an effective comparison among the different treatments (Figure 3.A) [26,33]. The tumor grafting procedure results in a high survival rate of the embryos, which permits the use of the system as a rough indicator of the treatment toxicity.…”

Section: Nas-cispt-tf2 Efficacy On Tumor-grafted Chorioallantoic Memb...mentioning

confidence: 99%

“…The tumor volumes were analyzed by the measurement of their length and width (Figures 3.B, S1.B, and Table S2) to calculate the tumor fold change (Figure S1.C) [26]. The comparison between the conditions has been performed by the analysis of the relative volume fold change (R-value) (Figure 3C, Table S3) in order to assess the effect of the treatments regardless the differences of the initial tumor size.…”

Section: Nas-cispt-tf2 Efficacy On Tumor-grafted Chorioallantoic Memb...mentioning

confidence: 99%

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Pro-apoptotic and size-reducing effects of protein corona-modulating nano-architectures enclosing platinum prodrug in in vivo oral carcinoma

et al. 2022

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Section: Cam Tumor Treatment and Monitoringmentioning

confidence: 97%

Section: Hematoxylin and Eosin (Hande) Stainingmentioning

confidence: 99%

Section: Nas-cispt-tf2 Efficacy On Tumor-grafted Chorioallantoic Memb...mentioning

confidence: 99%

Section: Nas-cispt-tf2 Efficacy On Tumor-grafted Chorioallantoic Memb...mentioning

confidence: 99%

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Pro-apoptotic and size-reducing effects of protein corona-modulating nano-architectures enclosing platinum prodrug in in vivo oral carcinoma

et al. 2022

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“…In order to overcome the resistance against cisplatin, biodegradable nanoarchitectures (NAs) were synthesized to encapsulate gold ultrasmall nanoparticles (USNPs) of approximately 3 nm in diameter together with a cisplatin prodrug as described previously [ 13 , 14 ]. These NAs consist of a hollow silica capsule which allow active molecules along polymer matrices with gold USNPs to reside in the inner cavity, and their rational design avoids the persistence of noble metals after the action [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Ultrasmall-in-Nano Architectures Loaded with Cisplatin Prodrug in Combination with Ionizing Radiation Induces DNA Damage and Apoptosis in Pancreatic Ductal Adenocarcinoma

Pei

Mapanao

Gregori

et al. 2022

Cancers

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Considering the dismal survival rate, novel therapeutic strategies are warranted to improve the outcome of pancreatic ductal adenocarcinoma (PDAC). Combining nanotechnology for delivery of chemotherapeutics—preferably radiosensitizing agents—is a promising approach to enhance the therapeutic efficacy of chemoradiation. We assessed the effect of biodegradable ultrasmall-in-nano architectures (NAs) containing gold ultra-small nanoparticles (USNPs) enclosed in silica shells loaded with cisplatin prodrug (NAs-cisPt) combined with ionizing radiation (IR). The cytotoxic effects and DNA damage induction were evaluated in PDAC cell lines (MIA PaCa2, SUIT2-028) and primary culture (PDAC3) in vitro and in the chorioallantoic membrane (CAM) in ovo model. Unlike NAs, NAs-cisPt affected the cell viability in MIA PaCa2 and SUIT2-028 cells. Furthermore, NAs-cisPt showed increased γH2AX expression up to 24 h post-IR and reduced β-globin amplifications resulting in apoptosis induction at DNA and protein levels. Similarly, combined treatment of NAs-cisPt + IR in PDAC3 and SUIT2-028 CAM models showed enhanced DNA damage and apoptosis leading to tumor growth delay. Our results demonstrate an increased cytotoxic effect of NAs-cisPt, particularly through its release of the cisplatin prodrug. As cisplatin is a well-known radiosensitizer, administration of cisplatin prodrug in a controlled fashion through encapsulation is a promising new treatment approach which merits further investigation in combination with other radiosensitizing agents.

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Hyperthermia Reduces Irradiation‐Induced Tumor Repopulation in an In Vivo Pancreatic Carcinoma Model

et al. 2023

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Pancreatic cancer has a poor prognosis due to its aggressive nature and ability to metastasize at an early stage. Currently, its management is still a challenge because this neoplasm is resistant to conventional treatment approaches, among which is chemo‐radiotherapy (CRT), due to the abundant stromal compartment involved in the mechanism of hypoxia. Hyperthermia, among other effects, counteracts hypoxia by promoting blood perfusion and thereby can enhance the therapeutic effect of radiotherapy (RT). Therefore, the establishment of integrated treatments would be a promising strategy for the management of pancreatic carcinoma. Here, the effects of joint radiotherapy/hyperthermia (RT/HT) on optimized chick embryo chorioallantoic membrane (CAM) pancreatic tumor models are investigated. This model enables a thorough assessment of the tumor‐arresting effect of the combined approach as well as the quantitative evaluation of hypoxia and cell cycle‐associated mechanisms by both gene expression analysis and histology. The analysis of the lower CAM allows to investigate the variation of the metastatic behaviors of the cancer cells associated with the treatments. Overall, this study provides a potentially effective combined strategy for the non‐invasive management of pancreatic carcinoma.

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Chorioallantoic membrane tumor models highlight the effects of cisplatin compounds in oral carcinoma treatment

Cited by 19 publications

References 73 publications

Pro-apoptotic and size-reducing effects of protein corona-modulating nano-architectures enclosing platinum prodrug in in vivo oral carcinoma

Pro-apoptotic and size-reducing effects of protein corona-modulating nano-architectures enclosing platinum prodrug in in vivo oral carcinoma

Biodegradable Ultrasmall-in-Nano Architectures Loaded with Cisplatin Prodrug in Combination with Ionizing Radiation Induces DNA Damage and Apoptosis in Pancreatic Ductal Adenocarcinoma

Hyperthermia Reduces Irradiation‐Induced Tumor Repopulation in an In Vivo Pancreatic Carcinoma Model

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