Choreography of multiple omics reveals the mechanism of lipid turnover in Schizochytrium sp. S31

Chang, Ming; Wang, Zhangtie; Li, Leilei; Lou, Fei; Ma, Meimei; Jin, Qingzhe; Wang, Xingguo

doi:10.1016/j.algal.2021.102182

Cited by 6 publications

(7 citation statements)

References 49 publications

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“…A late increase of PUFA content was reported also in Schizochytrium sp. S31 [ 8 ]. In this thraustochytrid, saturated fatty acids in neutral lipids were preferentially consumed, once the carbon supply in the culture medium was exhausted, to generate PUFA-rich phospholipids [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…S31 [ 8 ]. In this thraustochytrid, saturated fatty acids in neutral lipids were preferentially consumed, once the carbon supply in the culture medium was exhausted, to generate PUFA-rich phospholipids [ 8 ]. As phospholipids are main components of cell membranes, this response would allow production of new cells, or cells better adapted to survive famine.…”

Section: Discussionmentioning

confidence: 99%

“…CCTCC M209059, 39.09 Mb [ 29 ]; Schizochytrium sp. S31, 42.99 Mb [ 8 ]; and Thraustochytrium sp. ATCC 26185, 39 Mb [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thraustochytrids are heterotrophic single-celled eukaryotes found commonly in marine ecosystems, and many of them have been investigated as potential sustainable sources of DHA to replace fish oil derived DHA from wild-caught marine fish. Much interest has focused on the thraustochytrid genus Schizochytrium , as species within this genus are particularly rich in lipids (30–50% of dry biomass), having a high proportion of DHA [ 8 ]. Other thraustochytrid genera, especially Thraustochytrium spp., produce lipids with a more complex fatty acid profile that includes DHA, EPA, and arachidonic acid (ARA, C20:4n−6) [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Antarctic Thraustochytrids as Sources of Carotenoids and High-Value Fatty Acids

et al. 2021

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Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and carotenoids are needed as human dietary supplements and are essential components in commercial feeds for the production of aquacultured seafood. Microorganisms such as thraustochytrids are potential natural sources of these compounds. This research reports on the lipid and carotenoid production capacity of thraustochytrids that were isolated from coastal waters of Antarctica. Of the 22 isolates, 21 produced lipids containing EPA+DHA, and the amount of these fatty acids exceeded 20% of the total fatty acids in 12 isolates. Ten isolates were shown to produce carotenoids (27.4–63.9 μg/g dry biomass). The isolate RT2316-16, identified as Thraustochytrium sp., was the best producer of biomass (7.2 g/L in five days) rich in carotenoids (63.9 μg/g) and, therefore, became the focus of this investigation. The main carotenoids in RT2316-16 were β-carotene and canthaxanthin. The content of EPA+DHA in the total lipids (34 ± 3% w/w in dry biomass) depended on the stage of growth of RT2316-16. Lipid and carotenoid content of the biomass and its concentration could be enhanced by modifying the composition of the culture medium. The estimated genome size of RT2316-16 was 44 Mb. Of the 5656 genes predicted from the genome, 4559 were annotated. These included genes of most of the enzymes in the elongation and desaturation pathway of synthesis of ω-3 polyunsaturated fatty acids. Carotenoid precursors in RT2316-16 were synthesized through the mevalonate pathway. A β-carotene synthase gene, with a different domain organization compared to the gene in other thraustochytrids, explained the carotenoid profile of RT2316-16.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…CCTCC M209059, 39.09 Mb [ 29 ]; Schizochytrium sp. S31, 42.99 Mb [ 8 ]; and Thraustochytrium sp. ATCC 26185, 39 Mb [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antarctic Thraustochytrids as Sources of Carotenoids and High-Value Fatty Acids

et al. 2021

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“…EPA was derived from fungus Pythium irregular waste glycerol with a final concentration of 90 mg/L and productivity of 14.9 mg/L/day [ 133 ]. The algal species Schizochytrium limacinum SR21 developed with a DHA productivity of 0.51 g/L/day with waste glycerol [ 134 ].…”

Section: Glycerol Into Value-added Productsmentioning

confidence: 99%

Glycerol waste to value added products and its potential applications

Chilakamarry

Sakinah

Zularisam

et al. 2021

Syst Microbiol and Biomanuf

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Graphic abstract The rapid industrial and economic development runs on fossil fuel and other energy sources. Limited oil reserves, environmental issues, and high transportation costs lead towards carbon unbiased renewable and sustainable fuel. Compared to other carbon-based fuels, biodiesel is attracted worldwide as a biofuel for the reduction of global dependence on fossil fuels and the greenhouse effect. During biodiesel production, approximately 10% of glycerol is formed in the transesterification process in a biodiesel plant. The ditching of crude glycerol is important as it contains salt, free fatty acids, and methanol that cause contamination of soil and creates environmental challenges for researchers. However, the excessive cost of crude glycerol refining and market capacity encourage the biodiesel industries for developing a new idea for utilising and produced extra sources of income and treat biodiesel waste. This review focuses on the significance of crude glycerol in the value-added utilisation and conversion to bioethanol by a fermentation process and describes the opportunities of glycerol in various applications.

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