Chorda Tympani Responses in Two Inbred Strains of Mice With Different Taste Preferences

Frank, Marion E.; Blizard, David A.

doi:10.1016/s0031-9384(99)00071-2

Cited by 45 publications

(26 citation statements)

References 82 publications

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“…We chose NH 4 Cl as a standard stimulus for data correction to be consistent with our previous studies (Bachmanov et al, 1997;Inoue et al, 2001a,b;Li et al, 2001). Moreover, in our previous analyses (discussed in detail in Inoue et al, 2001a,b), we have shown that (1) NH 4 Cl responses normalized relative to responses to other taste stimuli are similar in the B6 and 129 strains, and (2) results of response normalization relative to NH 4 Cl are similar to normalization using an alternative approach proposed by Frank and Blizard (1999). An additional argument supporting the appropriateness of our standardization procedure is that in this study we observed genotypical differences in responses to only a subset of stimuli, rather than generalized differences that would be expected if they were confounded by the standardization procedure.…”

Section: Methodssupporting

confidence: 92%

Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F₂Hybrids between C57BL/6ByJ and 129P3/J Mice

Inoue¹,

Reed²,

Li³

et al. 2004

J. Neurosci.

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Recent studies have shown that the T1R3 receptor protein encoded by the Tas1r3 gene is involved in transduction of sweet taste. To assess ligand specificity of the T1R3 receptor, we analyzed the association of Tas1r3 allelic variants with taste responses in mice. In the F 2 hybrids between the C57BL/6ByJ (B6) and 129P3/J (129) inbred mouse strains, we determined genotypes of markers on chromosome 4, where Tas1r3 resides, measured consumption of taste solutions presented in two-bottle preference tests, and recorded integrated responses of the chorda tympani gustatory nerve to lingual application of taste stimuli. For intakes and preferences, significant linkages to Tas1r3 were found for the sweeteners sucrose, saccharin, and D-phenylalanine but not glycine. For chorda tympani responses, significant linkages to Tas1r3 were found for the sweeteners sucrose, saccharin, D-phenylalanine, D-tryptophan, and SC-45647 but not glycine, L-proline, L-alanine, or L-glutamine. No linkages to distal chromosome 4 were detected for behavioral or neural responses to non-sweet quinine, citric acid, HCl, NaCl, KCl, monosodium glutamate, inosine 5Ј-monophosphate, or ammonium glutamate. These results demonstrate that allelic variation of the Tas1r3 gene affects gustatory neural and behavioral responses to some, but not all, sweeteners. This study describes the range of ligand sensitivity of the T1R3 receptor using an in vivo approach and, to our knowledge, is the first genetic mapping study of activity in gustatory nerves.

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Section: Methodssupporting

confidence: 92%

Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F₂Hybrids between C57BL/6ByJ and 129P3/J Mice

Inoue¹,

Reed²,

Li³

et al. 2004

J. Neurosci.

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“…Moreover, in our previous analyses (discussed in detail in Refs. 27, 28), we showed that 1) NH 4Cl responses normalized relative to responses to other taste stimuli are similar in the B6 and 129 strains and 2) results of response normalization relative to NH 4Cl are similar to normalization using an alternative approach (21). An additional argument supporting the appropriateness of our standardization procedure is that in this study we observed genetic differences in responses to only a subset of stimuli, rather than generalized differences that would be expected if the standardization procedure were inappropriate.…”

Section: Experiments 3: Responses Of Chorda Tympani Nervementioning

confidence: 67%

Allelic variation of the Tas1r3 taste receptor gene selectively affects taste responses to sweeteners: evidence from 129.B6-Tas1r3 congenic mice

Inoue

Glendinning

Theodorides

et al. 2007

Physiological Genomics

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The Tas1r3 gene encodes the T1R3 receptor protein, which is involved in sweet taste transduction. To characterize ligand specificity of the T1R3 receptor and the genetic architecture of sweet taste responsiveness, we analyzed taste responses of 129.B6-Tas1r3 congenic mice to a variety of chemically diverse sweeteners and glucose polymers with three different measures: consumption in 48-h two-bottle preference tests, initial licking responses, and responses of the chorda tympani nerve. The results were generally consistent across the three measures. Allelic variation of the Tas1r3 gene influenced taste responsiveness to nonnutritive sweeteners (saccharin, acesulfame-K, sucralose, SC-45647), sugars (sucrose, maltose, glucose, fructose), sugar alcohols (erythritol, sorbitol), and some amino acids (D-tryptophan, D-phenylalanine, L-proline). Tas1r3 genotype did not affect taste responses to several sweet-tasting amino acids (L-glutamine, L-threonine, L-alanine, glycine), glucose polymers (Polycose, maltooligosaccharide), and nonsweet NaCl, HCl, quinine, monosodium glutamate, and inosine 5'-monophosphate. Thus Tas1r3 polymorphisms affect taste responses to many nutritive and nonnutritive sweeteners (all of which must interact with a taste receptor involving T1R3), but not to all carbohydrates and amino acids. In addition, we found that the genetic architecture of sweet taste responsiveness changes depending on the measure of taste response and the intensity of the sweet taste stimulus. Variation in the T1R3 receptor influenced peripheral taste responsiveness over a wide range of sweetener concentrations, but behavioral responses to higher concentrations of some sweeteners increasingly depended on mechanisms that could override input from the peripheral taste system.

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“…1. Therefore, measuring the amplitude at two different intervals, for example within the first 2 sec and at 10 sec, gives a better representation of the response [38,39]. …”

Section: Discussionmentioning

confidence: 99%

Comparison of the responses of the chorda tympani and glossopharyngeal nerves to taste stimuli in C57BL/6J mice

Danilova

Hellekant

2003

BMC Neurosci

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Background: Recent progress in discernment of molecular pathways of taste transduction underscores the need for comprehensive phenotypic information for the understanding of the influence of genetic factors in taste. To obtain information that can be used as a base line for assessment of effects of genetic manipulations in mice taste, we have recorded the whole-nerve integrated responses to a wide array of taste stimuli in the chorda tympani (CT) and glossopharyngeal (NG) nerves, the two major taste nerves from the tongue.

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Chorda Tympani Responses in Two Inbred Strains of Mice With Different Taste Preferences

Cited by 45 publications

References 82 publications

Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F₂Hybrids between C57BL/6ByJ and 129P3/J Mice

Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F₂Hybrids between C57BL/6ByJ and 129P3/J Mice

Allelic variation of the Tas1r3 taste receptor gene selectively affects taste responses to sweeteners: evidence from 129.B6-Tas1r3 congenic mice

Comparison of the responses of the chorda tympani and glossopharyngeal nerves to taste stimuli in C57BL/6J mice

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Chorda Tympani Responses in Two Inbred Strains of Mice With Different Taste Preferences

Cited by 45 publications

References 82 publications

Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F2Hybrids between C57BL/6ByJ and 129P3/J Mice

Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F2Hybrids between C57BL/6ByJ and 129P3/J Mice

Allelic variation of the Tas1r3 taste receptor gene selectively affects taste responses to sweeteners: evidence from 129.B6-Tas1r3 congenic mice

Comparison of the responses of the chorda tympani and glossopharyngeal nerves to taste stimuli in C57BL/6J mice

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Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F₂Hybrids between C57BL/6ByJ and 129P3/J Mice

Allelic Variation of theTas1r3Taste Receptor Gene Selectively Affects Behavioral and Neural Taste Responses to Sweeteners in the F₂Hybrids between C57BL/6ByJ and 129P3/J Mice