Choosing the tactics of surgical treatment of vascular leukomas based on impression cytology

Zlobin, I. A.; Churashov, Sergey V.; Куликов, А.Н.; Chirsky, V. S.; Chernysh, V F; Gavrilyuk, I. O.

doi:10.21516/2072-0076-2022-15-2-supplement-61-68

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Проведение последующего ROC-анализа позволило определить пороговые уровни этих показателей, которые могут быть использованы в качестве предикторов в комплексе диагностических критериев при скрининге пациентов в группе риска, при подозрении на кератоконус или при монолатеральном кератоконусе. Метод импрессионной цитологии с высокой достоверностью позволяет оценить in vivo морфологическую картину эпителия глазной поверхности путем анализа объективных качественных и количественных данных [14,15].…”

Section:  результатыunclassified

The Disorder of Ocular Surface Homeostasis in Keratoconus

Ситник,

Степанова

2024

Офтальмология. Восточная Европа

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Цель. Изучение цитоморфологических особенностей глазной поверхности у пациентов с кератоконусом различных стадий во взаимосвязи с экспрессией в слезной жидкости биохимических маркеров состояния экстрацеллюлярного матрикса и воспаления. Материалы и методы. Для проведения исследования были сформированы 3 группы. В 1-ю группу вошли 28 пациентов с кератоконусом I–III стадии, во 2-ю группу – 28 пациентов с кератоконусом IV стадии, 3-ю группу составили 28 здоровых добровольцев. Выполнены забор слезы и определение концентрации в ней биохимических показателей. У 15 пациентов (30 глаз) с различными стадиями кератоконуса был проведен забор материала путем аппликации стерильного мембранного ацетат-целлюлозного диска для импрессионной цитологии. Результаты. Выявлены различия в содержании в слезе маркеров воспаления (СРБ, ИЛ-1β, ИЛ-6, ИЛ-17, ФНО-α) при кератоконусе I–III стадии и кератоконусе IV стадии. Концентрация маркеров состояния экстрацеллюлярного матрикса (коллаген IV типа, ММП-9, альфа-2-макроглобулин) при кератоконусе достоверно отличается от таковой у здоровых добровольцев. Результаты импрессионной цитологии показали выраженные изменения роговичного эпителия и стромы, отражающие нарушение цитокин-опосредованных эпителиально-стромальных коммуникаций, что является морфологическим субстратом прогрессирования заболевания. Выводы. Определен диагностический комплекс маркеров кератоконуса, включающий изменение концентрации ММП-9, ИЛ-1β и ИЛ-17, α2-МГ в слезной жидкости, установлены их пороговые уровни. Purpose. To study the cytomorphological features of the ocular surface in patients with keratoconus in relation to the expression of biochemical markers of the extracellular matrix and inflammation in the tear fluid. Materials and methods. Three groups of patients were included in the study. Group 1 consisted of 28 patients with grade I–III keratoconus, group 2 included 28 patients with grade IV keratoconus, group 3 included 28 healthy volunteers. A tear samples were collected in all groups and the concentrations of biochemical markers were determined. The sterile membrane cellulose acetate disks for impression cytology were used for collecting material in 15 patients (30 eyes) with various grades of keratoconus. Results. The differences in the concentrations of inflammatory markers (CRP, IL-1β, IL-6, IL-17, TNF-α) in tears were revealed in grade I–III keratoconus and grade IV keratoconus. The concentration of markers of the state of the extracellular matrix (type IV collagen, MMP-9, alpha-2 macroglobulin) in keratoconus is significantly different from that in healthy volunteers. Impression cytology showed changes in the corneal epithelium and stroma illustrating disruption of cytokine-mediated epithelial-stromal communications, which is the morphological substrate of disease progression. Conclusions. A diagnostic complex of keratoconus markers including of MMP-9, IL-1β and IL-17, α2-MG in the tear fluid and their cut-off levels was determined.

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Section:  результатыunclassified

The Disorder of Ocular Surface Homeostasis in Keratoconus

Ситник,

Степанова

2024

Офтальмология. Восточная Европа

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Impression Cytology as a Method of Cytological Diagnostics in Ophthalmology. Literature Review

et al. 2023

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Impression cytology (from the Latin impressio — impact, indentation) is a minimally invasive method of cytological examination of the surface epithelium of the cornea, limbus and conjunctiva using various sorbing applicators for the collection of cellular material. The spectrum of diseases, in the diagnosis of which impression cytology is traditionally used, is mainly associated with pathological processes occurring in the conjunctiva. Such diseases as: Sjogren’s syndrome, dry eye syndrome, mucopolysaccharidosis, vitamin A deficiency, trachoma, squamous cell neoplasia of the ocular surface, etc. With the advent of the concept of limbal epithelial stem cell, new diagnostic possibilities of impression cytology in ophthalmology occurred.

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Features and Outcomes of Penetrating Keratoplasty in Combined Opto-reconstructive Surgery for Combat Eye Injury and its Complication

Kulikov,

Churashov,

Danilenko

et al. 2024

Oftalʹmologiâ

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Purpose: to evaluate the features and outcomes of penetrating keratoplasty in combined opto-reconstructive surgery for combat eye injury and its complications.Patients and methods. The study enrolled 17 eyes (16 men) aged 34.00 ± 12.51 years, divided into 2 groups: I — with inflammatory diseases of the anterior segment, complicated combat eye injury (4 eyes), II — with open eye injury (OGI, 13 eyes). Patients underwent examination to reveal and localize damaged intraocular structures and intraocular foreign bodies (IOFB), determine the prognosis of visual functions, as well as impression cytology. In all cases penetrating keratoplasty was performed, as well as complex of vitreoretinal surgery if it was needed. In the postoperative period, visual acuity, the timing of epithelialization, and graft transparency were assessed. Period of observation was 4,5 ± 2,1 months.Results. Group I had severe keratouveitis, when intraocular structures were intact also as electrophysiological parameters. The presence of mucosal cells was determined in 50 % of cases. The number of surgery was 2.00 ± 0.82. Graft epithelialization was completed in 38.99 ± 35.43 days. Postoperatively all grafts had transparent retention and complete epithelialization, also as had high visual acuity (0.25 ± 0.10) was noted. Group II had damaged several intraocular structures and varying electrophysiological parameters. The epithelial phenotype was corneal all over. The average number of surgical treatments was 3.00 ± 1.48. The time of graft epithelialization was significantly less — 17.66 ± 8.69 days (p = 0.014). Corrected visual acuity ranged from zero (30.77 %) to 0.3 (0.08 ± 0.11) and was significantly less than in group I (p = 0.018). The parameters that correlated low visual acuity were aphakia, the level of electrical sensitivity, the number of surgical interventions, and the age of patients.Conclusions. Penetrating keratoplasty functional results prognosis is better in group with infectious complications of combat eye injury with reduced cornea transparency than in patient with open globe injury. Besides light perception and conducting structures (retina, optical nerve) lesion, optico-reconstructive surgery in open globe injury often leads to single-chamber formation in silicone filled eye, which significantly worsens the functional prognosis of treatment.

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Choosing the tactics of surgical treatment of vascular leukomas based on impression cytology

Cited by 3 publications

References 6 publications

The Disorder of Ocular Surface Homeostasis in Keratoconus

The Disorder of Ocular Surface Homeostasis in Keratoconus

Impression Cytology as a Method of Cytological Diagnostics in Ophthalmology. Literature Review

Features and Outcomes of Penetrating Keratoplasty in Combined Opto-reconstructive Surgery for Combat Eye Injury and its Complication

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