Chondroprotective effects of a polycarbonate-urethane meniscal implant: histopathological results in a sheep model

Zur, Gal; Linder-Ganz, Eran; Elsner, Jonathan J.; Shani, Jonathan; Brenner, Ori; Agar, Gabriel; Hershman, Elliott B.; Arnoczky, Steven P.; Guilak, Farshid; Shterling, Avi

doi:10.1007/s00167-010-1210-5

Cited by 99 publications

(84 citation statements)

References 42 publications

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“…The anatomy of the ovine stifle joint has been well described and is considered a reasonable model of the human knee joint [20,21]. It has been used to investigate articular cartilage repair as well as anterior cruciate ligament (ACL) reconstruction [22] and meniscal repair [23] and replacement [24]. Caplan et al (2011) proposed the idea that MSCs work using a paracrine effect, essentially acting as a 'drug store' to aid in tissue regeneration [25].…”

Section: Introductionmentioning

confidence: 99%

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Hindle

Baily

Khan

et al. 2016

Stem Cells and Development

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Publisher Rights Statement:This is the final peer-reviewed manuscript as accepted for publication General rightsCopyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policyThe University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact openaccess@ed.ac.uk providing details, and we will remove access to the work immediately and investigate your claim. Immunohistochemistry and fluorescence-activated cell sorting (FACS) were used to ascertain the reactivity of anti-human and anti-ovine antibodies, which were combined and used to identify and isolate pericytes (CD34-CD45-CD146+) and adventitial cells (CD34+CD45-CD146-). The purified cells demonstrated osteogenic, adipogenic and chondrogenic potential in culture.Autologous ovine PSCs (oPSCs) were isolated, cultured and efficiently transfected using a GFP encoding lentivirus. The cells were implanted into articular cartilage defects on the medial femoral condyle using a hydrogel and collagen membranes.Four weeks following implantation the condyle was explanted and confocal laser scanning microscopy demonstrated the presence of oPSCs in the defect repaired with the hydrogel.These data suggest the testability in a large animal of native mesenchymal stem cell autologous grafting, thus avoiding possible biases associated with xenotransplantation.Such a setting will be used in priority for indications in orthopaedics, at first to model articular cartilage repair.

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Section: Introductionmentioning

confidence: 99%

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Hindle

Baily

Khan

et al. 2016

Stem Cells and Development

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“…The computed contact mechanics under the ultimate implant resembled those of an intact human meniscus measured from cadaveric experiments [51,52]. Subsequent in vivo experiments in a sheep model showed no differences in cartilage condition between the PCU implant and the contralateral control joints up to six months post-operatively [53]. However, Mankin scores of these non-operated controls were relatively high compared to earlier reported values [54], and it would be interesting to see if the chondroprotective effect is prolonged upon longterm implantation.…”

Section: Synthetic Total Meniscal Substitutesmentioning

confidence: 75%

“…Publications on the design and testing processes of the disc-shaped, free-floating NUsurface® implant all originate from recent years [51][52][53]. The short span of time of these publications and the fact that the implant has already proceeded to phase 1 clinical trials illustrate the high speed at which progress can be made with synthetic implants that do not require tissue regeneration.…”

Section: Future Directions In Meniscus Replacementmentioning

confidence: 99%

“…3b), the human version is disc shaped and freefloating (Fig. 3a) [51,53]. Arguments for this change were not provided in the literature and no publications are available describing the effects of the free-floating implant in animal studies.…”

Section: Synthetic Total Meniscal Substitutesmentioning

confidence: 99%

“…b Anatomically shaped version of the NUsurface® total meniscal prosthesis, including fixation bolts at both horns. Reprinted from [53]. c A 12-month follow-up sagittal MR image of the NUsurface® meniscal replacement.…”

Section: Preclinical Testing Platformsmentioning

confidence: 99%

See 2 more Smart Citations

Synthetic meniscus replacement: a review

Vrancken

Buma

Tienen

2012

International Orthopaedics (SICOT)

105

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Current Concepts in Meniscus Tissue Engineering and Repair

Bilgen

Jayasuriya

Owens

2018

Adv Healthcare Materials

108

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The meniscus is the most commonly injured structure in the human knee. Meniscus deficiency has been shown to lead to advanced osteoarthritis (OA) due to abnormal mechanical forces, and replacement strategies for this structure have lagged behind other tissue engineering endeavors. The challenges include the complex 3D structure with individualized size parameters, the significant compressive, tensile and shear loads encountered, and the poor blood supply. In this progress report, a review of the current clinical treatments for different types of meniscal injury is provided. The state-of-the-art research in cellular therapies and novel cell sources for these therapies is discussed. The clinically available cell-free biomaterial implants and the current progress on cell-free biomaterial implants are reviewed. Cell-based tissue engineering strategies for the repair and replacement of meniscus are presented, and the current challenges are identified. Tissue-engineered meniscal biocomposite implants may provide an alternative solution for the treatment of meniscal injury to prevent OA in the long run, because of the limitations of the existing therapies.

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Chondroprotective effects of a polycarbonate-urethane meniscal implant: histopathological results in a sheep model

Abstract: current findings provide preliminary evidence for the ability of an artificial PCU meniscal implant to delay or prevent osteoarthritic changes in knee joint following complete medial meniscectomy.

Cited by 99 publications

References 42 publications

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Synthetic meniscus replacement: a review

Current Concepts in Meniscus Tissue Engineering and Repair

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