Chondroitin Sulfates and Their Role in Metobolism of Chondrocytes and Intercellular Matrix of Cartilage Tissue

Panasvuk, A F; Larionov, E V

doi:10.14412/1995-4484-2000-1233

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…Результаты научных исследований, выполненных в последние десятилетия, позволили сформировать понимание о полифункциональном влиянии сульфатированных гликозаминогликанов (сГАГ) [3][4][5][6]. Экзогенно введенные сГАГ могут оказывать трофическую и структурную функции в тканях, а также оказывать регуляторное действие на многие физиологические и патологические процессы, в том числе на пролиферацию и дифференциацию клеток [7].…”

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The effect of the sulfated glycosaminoglycans complex on the human corneal epithelial cells proliferation: an <i>in vitro</i> experiment

Trifanenkova

Petersen

Novikov³

et al. 2022

Aspirantskiy Vestnik Povolzhiya

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Aim to study the proliferative effect of various concentrations of sulfated glycosaminoglycans (sGAG) on the culture of human corneal epithelial cells. Material and methods. The study focused on the properties of a mixture of sGAG consisting of chondroitin-4-sulfate, chondroitin-6-sulfates and keratan sulfates obtained by isolation from the transparent unchanged stroma of the cornea of farm animals. The material for the in vitro study was the cells of the anterior epithelium of the human cornea. In the experimental group No.1, a mixture of sGAG was added to the culture medium at a concentration of 0.1%, in the experimental group No.2 at a concentration of 0.5%, in the experimental group No.3 at a concentration of 1.0%. In the control group No.1, the medium was replaced without the addition of solutions, cell seeding was carried out similarly to seeding with experimental samples. The negative control group included the holes of the tablet without the addition of medium and solution. Results. The highest levels of the cellular index (CI) were obtained in the experimental group No.3 (1.00% of sGAG) and amounted to 2.86 0.11 (the total increase in indicators was 60.67%). In the experimental group No.2 (0.50% sGAG), the cellular index levels were 2.650.24 (the total increase in indicators was 52.29%), which was significantly different from the experimental group No.1 (0.10% sGAG) and the control group No.1, where these indicators were 2.54 0.21 (35.81%) and 2.370.02 (25.39%) respectively. Conclusion. The final CI indicators show that solutions of the sGAG mixture at concentrations of 0.5% and 1.0% have a more pronounced proliferative effect on human corneal epithelial cells in vitro from the range of concentrations under study. The results obtained open up the prospects for the use of sGAG in the development of new approaches to the treatment of patients undergoing a chronic drug therapy or patients with concomitant corneal diseases.

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Section: Introductionunclassified

The effect of the sulfated glycosaminoglycans complex on the human corneal epithelial cells proliferation: an <i>in vitro</i> experiment

Trifanenkova

Petersen

Novikov³

et al. 2022

Aspirantskiy Vestnik Povolzhiya

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Prescription of chondroitin sulfate in back pains

Shavlovskaya

Романов

2020

Medicinskij sovet

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One of the promising areas of pharmacotherapy in degenerative-dystrophic lesions of the joints, such as osteoarthritis (OA), is the use of chondroprotectors (CP). CPs belong to the group of Symptomatic Slow Acting Drugs for OsteoArthritis (SYSADOA). Among CPs, chondroitin sulfate (CS) is considered to be the most acceptable means capable of influencing metabolic processes in cartilage, synovial and bone tissue, suppressing the synthesis of proinflammatory mediators. CS improves phosphorus-calcium metabolism in cartilage tissue, inhibits enzymes that violate the structure and function of articular cartilage, inhibits the degeneration of cartilage tissue; stimulates the synthesis of glycosaminoglycans (GAG), normalizes the metabolism of hyaline tissue, promotes regeneration of cartilage surfaces and articular bags.The main effects that show CS on the joint in OA: anti-inflammatory, analgesic, protective. Slowing bone resorption, reduces the loss of Ca2+ and accelerates the processes of bone repair, inhibits the progression of OA. It has analgesic effect, reduces joint pain, pain at rest and when walking, the severity of inflammation, reduces the need for non-steroidal anti-inflammatory drugs (NSAIDs). The appointment of correctors of bone and cartilage metabolism is indicated at any stage of the degenerative process in the joints. One of the medicinal product (MP) based on CS is Chondroitin-ACOS (capsules, 25mg), whose effectiveness in the treatment of patients with OA has been demonstrated in a number of studies. Dosage Chondroitin-ACOS: 0,75 g 3 times a day for 3 weeks, then 0,5 g 2 times a day for 9 weeks. The therapeutic effect develops after 8-12 weeks of taking the drug. Within one month after discontinuation of Chondroitin-ACOS, its aftereffect persisted. Chondroitin-ACOS can be recommended for patients with severe pain syndrome, radiologically confirmed OA I-III stages of different localization.

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Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon

Chepkasova

Slutskaya

Караулова

2022

Izv. Tihookean. naučno-issled. rybohoz. centra

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Аннотация. Из хрящевой ткани ската и осетра (калуги) получены сульфатированные гликозаминогликаны (ГАГ) -хондроитинсульфаты -при следующих условиях: гидротермическая обработка сырья при 50 о С; гидролиз протеолитическим ферментом (гидромодуль 1 : 1; температура 45 о С; длительность 4 ч), последовательное осаждение ГАГ спиртом (2-3 объемами этилового спирта (96 о ) с высаливанием 2 %-ным раствором хлорида натрия. В полученных образцах определено содержание гексозаминов и сульфатированных ГАГ: установлено, что ГАГ из ската и калуги отличаются высоким содержанием сульфатированных форм, сравнимым с коммерческим препаратом, что открывает перспективы разработки технологии получения из отходов при разделке этих объектов монокомпонентных биологически активных добавок хондропротекторного действия.

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Chondroitin Sulfates and Their Role in Metobolism of Chondrocytes and Intercellular Matrix of Cartilage Tissue

Cited by 4 publications

References 15 publications

The effect of the sulfated glycosaminoglycans complex on the human corneal epithelial cells proliferation: an <i>in vitro</i> experiment

The effect of the sulfated glycosaminoglycans complex on the human corneal epithelial cells proliferation: an <i>in vitro</i> experiment

Prescription of chondroitin sulfate in back pains

Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon

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