Chondroitin sulfate is involved in the hypercalcification of the organic matrix of bovine peritubular dentin

Dorvee, Jason R.; Gerkowicz, Lauren; Bahmanyar, Sara; Deymier-Black, Alix C.; Veis, Arthur

doi:10.1016/j.archoralbio.2015.11.008

Cited by 12 publications

(3 citation statements)

References 13 publications

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“…However, it is well documented that dentin mineral is evenly distributed in dentin matrix with many hypermineralized collars (known as the peritubular dentin) surrounding odontoblast processes, indicating a likely mechanism by which mineralization occurs through odontoblast processes. Furthermore, a sulfated component (chondroitin sulfate) was recently identified within the hypermineralized collars of the organic matrix of bovine peritubular dentin 11 . These authors speculate that the presence of sulfur co-localized with calcium may sequester calcium ions allowing for the entire complex to later become mineralized.…”

Section: Introductionmentioning

confidence: 99%

Dentinal mineralization is not limited in the mineralization front but occurs along with the entire odontoblast process

Jing

Wang

et al. 2018

Int. J. Biol. Sci.

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The mineralization-front theory is historically rooted in mineralization research fields for many decades. This theory is widely used to describe mineralization events in both osteogenesis and dentinogenesis. However, this model does not provide enough evidence to explain how minerals are propagated from the pulp-end dentin to dentin-enamel junction (DEJ). To address this issue, we modified the current research approaches by a) extending the mineral deposition windows of time from minutes to hours, instead of limiting the mineralization assay on days and weeks only; b) switching a regular fluorescent microscope to a more powerful confocal microscope; in which both mineral deposition rates and detail mineral labeling along with dentin tubules can be documented; and c) using reporter mice, including the Gli1-CreERT2 activated tomato and the 2.3 Col1-GFP to mark odontoblast processes combined with mineral dye injections. Our key findings are: 1) Odontoblast-processes, full of numerous mini-branches, evenly spread to entire dentin matrices with a high density of processes and a large diameter of the main process at the predentin-dentin junction; and 2) The minerals deposit along with entire odontoblast-processes and form many individual mineral collars surrounding odontoblast processes. As a result, these merged collars give rise to a single labeled line at the dentin-predentin junction, in which the dental tubules are wider in diameter and denser in odontoblast processes compared to other dentin areas. We therefore propose that it is the odontoblast-process that directly contributes to mineralization, which is not simply limited in the mineralization front at the edge of dentin and predentin, but occurs along with the entire odontoblast process. These new findings will shed new light on our understanding of dentin structure and function, as well as the mechanisms of mineralization.

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Section: Introductionmentioning

confidence: 99%

Dentinal mineralization is not limited in the mineralization front but occurs along with the entire odontoblast process

Jing

Wang

et al. 2018

Int. J. Biol. Sci.

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“…GAGs are also thought to play a role in the mineralization; in dentine, chondroitin sulfate may allow the mineralization process through sequestering calcium ions. These mechanisms might be displayed in other mineralized tissues [99][100][101][102][103].…”

Section: Slrp Gag Moieties: Fingerprints Of the Tissue Status And Actmentioning

confidence: 97%

From Translation to Protein Degradation as Mechanisms for Regulating Biological Functions: A Review on the SLRP Family in Skeletal Tissues

Zappia¹,

Joiret

Sanchez³

et al. 2020

Biomolecules

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The extracellular matrix can trigger cellular responses through its composition and structure. Major extracellular matrix components are the proteoglycans, which are composed of a core protein associated with glycosaminoglycans, among which the small leucine-rich proteoglycans (SLRPs) are the largest family. This review highlights how the codon usage pattern can be used to modulate cellular response and discusses the biological impact of post-translational events on SLRPs, including the substitution of glycosaminoglycan moieties, glycosylation, and degradation. These modifications are listed, and their impacts on the biological activities and structural properties of SLRPs are described. We narrowed the topic to skeletal tissues undergoing dynamic remodeling.

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“…The PTD is irregular and not uniformly distributed around the dentine tubules (DTs). The texture of the PTD mineral is different than that of the ITD mineral, and it is difficult to suggest any function of PTD, besides it could provides for the transport of ions and other components [ 8 ]. Around tubules, the collagen fibrils are randomly distributed in intertubular dentine.…”

Section: Introductionmentioning

confidence: 99%

The peritubular reinforcement effect of porous dentine microstructure

Wang¹,

Niu²,

Li³

et al. 2017

PLoS ONE

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In the current study, we evaluate the equivalent stiffness of peritubular reinforcement effect (PRE) of porous dentine optimized by the thickness of peritubular dentine (PTD). Few studies to date have evaluated or quantitated the effect of PRE on composite dentine. The miscrostructure of porous dentine is captured by scanning electron microscope images, and then finite element modeling is used to quantitate the deformation and stiffness of the porous dentine structure. By optimizing the radius of PTD and dentine tubule (DT), the proposed FE model is able to demonstrate the effect of peritubular reinforcement on porous dentine stiffness. It is concluded that the dentinal equivalent stiffness is reduced and degraded with the increase of the radius of DT (i.e., porosity) in the certain ratio value of Ep/Ei and certain radius of PTD, where Ep is the PTD modulus and Ei is the intertubular dentine modulus. So in order to ensure the whole dentinal equivalent stiffness is not loss, the porosity should get some value while the Ep/Ei is certain. Thus, PTD prevents the stress concentration around DTs and reduces the risk of DTs failure. Mechanically, the overall role of PTD appears to enhance the stiffness of the dentine composite structure. These results provide some new and significant insights into the biological evolution of the optimal design for the porous dentine microstructure. These findings on the biological microstructure design of dentine materials are applicable to other engineering structural designs aimed at increasing the overall structural strength.

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Chondroitin sulfate is involved in the hypercalcification of the organic matrix of bovine peritubular dentin

Cited by 12 publications

References 13 publications

Dentinal mineralization is not limited in the mineralization front but occurs along with the entire odontoblast process

Dentinal mineralization is not limited in the mineralization front but occurs along with the entire odontoblast process

From Translation to Protein Degradation as Mechanisms for Regulating Biological Functions: A Review on the SLRP Family in Skeletal Tissues

The peritubular reinforcement effect of porous dentine microstructure

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