Chondroitin Sulfate‐Depolymerizing Activity in <i>Streptococcus intermedius</i> and Other Streptococci

Osano, E; Hibi, E; Ozeki, Masami; Fujii, Yoshiro; Moriyama, Takafumi

doi:10.1111/j.1348-0421.1987.tb01346.x

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…The chondroitinase of P. acnes ATCC 11828 was much more active than that of S. intermedins OE-1 and the activ ity was not detected in S. mutans OZ-1 or strain Ingbritt (serotype c) [Osano et al, 1987]. If the enzyme did work in the affected dentin, alcinophilic areas which appeared af ter proteolytic digestion of proteoglycan in the dentin ma trix must have decreased or diminished because of degra dation of chondroitin sulfate(s).…”

Section: Discussionmentioning

confidence: 99%

“…S. intermedius OE-1 was isolated from supra gingival plaque from a sound tooth of one of us using the method of Tanaka [1988] and identified according to the VPI manual [Holde man et al, 1977], The strain was serologically classified into the same scrogroup as strain ATCC 27335 [Osano et al. 1990], P. acnes ATCC 11828 possessed chondroitinase activity [Osano et al. 1987],…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Streptococcus interme dins and others have no clear standing in taxonomy [Coykendall et al, 1987;Whilcy and Beighton, 1991]. They be long to the 'Streptococcus anginosus-milleri group' accord ing to Bergey's Manual [Butler, 1986], Osano et al [1987], however, demonstrated that S. intermedins ATCC 27335 was the sole producer of chondroitinase among the spe cies of the S. anginosus-milleri group. By screening the chondroitinase activity of anaerobically grown isolates from approximal and from supra-and subgingival plaque of healthy dental students, Tanaka [1988] obtained a num ber of isolates which resembled S. intermedius ATCC 27335 in terms of morphology and sugar fermentation pattern according to the VPI manual [Holdeman et al, 1977],…”

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confidence: 99%

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Invasion of Streptococcus mutans, Streptococcus intermedius and Propionibacterium acnes into the Teeth of Gnotobiotic Rats

Kobayashi¹,

Ozeki²,

Ogawa³

et al. 1992

Caries Res

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Germ-free rats, fed a modified cariogenic diet 2000 with a reduced sucrose content of 25%, were inoculated with a strain of Streptococcus mutans, Streptococcus intermedius or Propionibacterium acnes, respectively. After 100 days on the diet, the rats were sacrificed. Microscopic examination of stained decalcified tooth sections revealed marked destruction of the outer zones of the dentin infected with S. mutans. The destruction was closely associated with the formation of glucan-mediated large aggregates of S. mutans. The aggregates, however, were rarely formed in the deep zones of the infected dentin. Inoculation with S. intermedius and P acnes had little effect on the microscopic outline of the decalcified tooth sections. However, the staining behavior of the sections with Mallory’s method, Alcian blue-PAS and silver was like that of the tooth sections infected with S. mutans, even in the central and inner zones of the dentin. The histochemical alterations of the deep zones of the dentin induced by the three infected bacteria appeared to be due to their proteolytic rather than to their acidogenic activities.

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Section: Discussionmentioning

confidence: 99%

Section: Bacterial Strainsmentioning

confidence: 99%

mentioning

confidence: 99%

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Invasion of Streptococcus mutans, Streptococcus intermedius and Propionibacterium acnes into the Teeth of Gnotobiotic Rats

Kobayashi¹,

Ozeki²,

Ogawa³

et al. 1992

Caries Res

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“…Production of ribonuclease, deoxyribonuclease, gelatinase, chondroitin sulfatase and collagenase has been demonstrated in 5. m///<?n Steffen, 1981;Osano, 1987;, but protein and lipid-degrading activities were not found . Hyaluronidase activity was observed mainly in 8-haemolytic strains .…”

Section: Pathogenicitymentioning

confidence: 99%

Streptococcus milleri : relevance of species

Jacobs¹

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Cloning and expression in Escherichia coli of the gene encoding the Proteus vulgaris chondroitin ABC lyase

Sato¹,

Shimada²,

Nakajima³

et al. 1994

Appl Microbiol Biotechnol

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The structural gene encoding chondroitin ABC lyase from Proteus vulgaris was cloned and sequenced. This gene consists of a single open reading frame of 3,063 bp, including a sequence (72 bp) for a possible secretory protein leader peptide, preceded by a Shine-Dalgarno ribosomal binding site. Promoter-like and rho-independent terminator sequences were detected upstream and downstream of the open reading frame, respectively. The G+C content of the coding region was 38.6%. The transcription startpoint was located 41-bp upstream of the initiation codon (ATG). Chondroitin ABC lyase is composed of 997 amino acids, and has a relative molecular mass of 112,635. When the 5.2-kb fragment containing the 1.2-kb upstream from the gene was inserted into pSTV29, and cloned in Escherichia coli, chondroitin ABC lyase was induced in the medium containing chondroitin-6-sulfate as the carbon source. On the other hand, when a 4.2-kb fragment containing only 0.2 kb upstream was inserted into pSTV29(pCHS delta 6), and pCHS delta 6 was introduced into E. coli, the enzyme was constitutively produced, even in medium containing glucose as the carbon source. By immunoblot analysis, the polypeptide synthesized by E. coli cells carrying pCHS delta 6 appeared to be the same as that of the purified chondroitin ABC lyase from P. vulgaris.

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Chondroitin Sulfate‐Depolymerizing Activity in Streptococcus intermedius and Other Streptococci

Cited by 6 publications

References 14 publications

Invasion of Streptococcus mutans, Streptococcus intermedius and Propionibacterium acnes into the Teeth of Gnotobiotic Rats

Invasion of Streptococcus mutans, Streptococcus intermedius and Propionibacterium acnes into the Teeth of Gnotobiotic Rats

Streptococcus milleri : relevance of species

Cloning and expression in Escherichia coli of the gene encoding the Proteus vulgaris chondroitin ABC lyase

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