Chondrogenic phenotype in responses to poly(ɛ-caprolactone) scaffolds catalyzed by bioenzymes: effects of surface topography and chemistry

Kosorn, Wasana; Sakulsumbat, Morakot; Lertwimol, Tareerat; Thavornyutikarn, Boonlom; Uppanan, Paweena; Chantaweroad, Surapol; Janvikul, Wanida

doi:10.1007/s10856-019-6335-6

Cited by 11 publications

(5 citation statements)

References 41 publications

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“…Jung et al (2008) reported enhanced deposition of chondral extracellular matrix onto LCM [38]. Kosorn et al (2019) treated the surface of LCM grafts enzymatically and reached higher hydrophilicity and improved chondrocyte proliferation and function [39]. Here, we did not study the growth and proliferation of chondrocytes, but of MSC.…”

Section: Discussionmentioning

confidence: 94%

Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity

et al. 2021

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In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity.

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Section: Discussionmentioning

confidence: 94%

Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity

et al. 2021

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“…Immunofluorescence staining of Sox9 showed that the 10–20% D/S group had higher expression than the 30% D/S group ( Appendix A , Appendix A ). These results suggested that 30% D/S group with excessive stiffness and smooth surface is unsuitable for chondrocyte growth and chondrogenesis [ 35 , 36 ]. On the other hand, almost all BMSCs were alive on the P/S materials, verifying the excellent biocompatibility of this scaffold ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Marginal sealing around integral bilayer scaffolds for repairing osteochondral defects based on photocurable silk hydrogels

Zhou

Jiang

et al. 2021

Bioactive Materials

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Osteochondral repair remains a major challenge in current clinical practice despite significant advances in tissue engineering. In particular, the lateral integration of neocartilage into surrounding native cartilage is a difficult and inadequately addressed problem that determines the success of tissue repair. Here, a novel design of an integral bilayer scaffold combined with a photocurable silk sealant for osteochondral repair is reported. First, we fabricated a bilayer silk scaffold with a cartilage layer resembling native cartilage in surface morphology and mechanical strength and a BMP-2-loaded porous subchondral bone layer that facilitated the osteogenic differentiation of BMSCs. Second, a TGF-β3-loaded methacrylated silk fibroin sealant (Sil-MA) exhibiting biocompatibility and good adhesive properties was developed and confirmed to promote chondrocyte migration and differentiation. Importantly, this TGF-β3-loaded Sil-MA hydrogel provided a bridge between the cartilage layer of the scaffold and the surrounding cartilage and then guided new cartilage to grow towards and replace the degraded cartilage layer from the surrounding native cartilage in the early stage of knee repair. Thus, osteochondral regeneration and superior lateral integration were achieved in vivo by using this composite. These results demonstrate that the new approach of marginal sealing around the cartilage layer of bilayer scaffolds with Sil-MA hydrogel has tremendous potential for clinical use in osteochondral regeneration.

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“…On the other hand, the material roughness may also contribute to determine the chondrocyte behavior. However, it has been demonstrated that its role is more relevant when assessing the glycosaminoglycans and collagen formation for Ra values up to 230 nm . Zinc deficiency has been correlated to cartilage-related diseases; being involved in the antioxidant capacity of chondrocytes, its lack might inhibit cell proliferation. , It has been demonstrated that zinc, even at low doses (lower than 0.5 μM), can increase the proliferation of chondrocytes by 40–50% …”

Section: Resultsmentioning

confidence: 99%

Novel Ultrathin Films Based on a Blend of PEG-b-PCL and PLLA and Doped with ZnO Nanoparticles

Vannozzi

Gouveia

Pingue

et al. 2020

ACS Appl. Mater. Interfaces

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In this paper, a novel nanofilm type is proposed based on a blend of poly(ethylene glycol)-block-poly(ε-caprolactone) methyl ether (PEG-b-PCL) and poly(l-lactic acid), doped with zinc oxide nanoparticles (ZnO NPs) at different concentrations (0.1, 1, and 10 mg/mL). All nanofilm types were featured by a thickness value of ∼500 nm. Increasing ZnO NP concentrations implied larger roughness values (∼22 nm for the bare nanofilm and ∼67 nm for the films with 10 mg/mL of NPs), larger piezoelectricity (average d 33 coefficient for the film up to ∼1.98 pm/V), and elastic modulus: the nanofilms doped with 1 and 10 mg/mL of NPs were much stiffer than the nondoped controls and nanofilms doped with 0.1 mg/mL of NPs. The ZnO NP content was also directly proportional to the material melting point and crystallinity and inversely proportional to the material degradation rate, thus highlighting the stabilization role of ZnO particles. In vitro tests were carried out with cells of the musculoskeletal apparatus (fibroblasts, osteoblasts, chondrocytes, and myoblasts). All cell types showed good adhesion and viability on all substrate formulations. Interestingly, a higher content of ZnO NPs in the matrix demonstrated higher bioactivity, boosting the metabolic activity of fibroblasts, myoblasts, and chondrocytes and enhancing the osteogenic and myogenic differentiation. These findings demonstrated the potential of these nanocomposite matrices for regenerative medicine applications, such as tissue engineering.

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Chondrogenic phenotype in responses to poly(ɛ-caprolactone) scaffolds catalyzed by bioenzymes: effects of surface topography and chemistry

Cited by 11 publications

References 41 publications

Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity

Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity

Marginal sealing around integral bilayer scaffolds for repairing osteochondral defects based on photocurable silk hydrogels

Novel Ultrathin Films Based on a Blend of PEG-b-PCL and PLLA and Doped with ZnO Nanoparticles

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