2006
DOI: 10.1016/j.joca.2005.09.002
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Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture

Abstract: These findings suggest that while MSCs do generate constructs with substantial cartilaginous properties, further optimization must be done to achieve levels similar to those produced by chondrocytes.

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Cited by 477 publications
(494 citation statements)
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“…Likewise, bovine MSCs show a sharp decrease with age in cartilage matrix-forming capacity that is more severe than reported for human MSCs in this same format [5]. Overall, at each age, and under ideal conditions (absence of TGF for chondrocytes, presence of TGF for MSCs), bovine chondrocytes in pellet culture produce more GAG and collagen than MSCs, consistent with our previous findings [17,22,33]. Taken together, when considering an autologous cellbased tissue engineering strategy for cartilage repair, age must be an important consideration.…”
Section: Discussionsupporting
confidence: 88%
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“…Likewise, bovine MSCs show a sharp decrease with age in cartilage matrix-forming capacity that is more severe than reported for human MSCs in this same format [5]. Overall, at each age, and under ideal conditions (absence of TGF for chondrocytes, presence of TGF for MSCs), bovine chondrocytes in pellet culture produce more GAG and collagen than MSCs, consistent with our previous findings [17,22,33]. Taken together, when considering an autologous cellbased tissue engineering strategy for cartilage repair, age must be an important consideration.…”
Section: Discussionsupporting
confidence: 88%
“…Interestingly, fetal and juvenile chondrocyte pellets in chemically defined medium with TGF-b3 accumulated less GAG and collagen (fetal only) than those cultured without TGF-b3, a result that has not been previously reported. In contrast, TGF-b3 improved both GAG content and mechanical properties for immature chondrocytes in the context of three-dimensional agarose hydrogels [33]. This may indicate a microenvironmental influence (such as cell-to-cell contact) in the interpretation of this soluble factor.…”
Section: Discussionmentioning
confidence: 90%
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