Chondrogenesis of human umbilical cord-derived mesenchymal stem cells in silk-fibroin/hyaluronic acid scaffolds for cartilage tissue engineering

Jaipaew, Jirayut; Wangkulangkul, Piyanun; Suwanrath, Chitkasem; Meesane, Jirut; Krivimol, Supaporn; Puttawibul, Puttisak

doi:10.1016/j.jbiotec.2014.07.428

Cited by 2 publications

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“…Combining the properties of natural protein and polysaccharides can be a promising strategy to obtain bioactive materials with a controlled structure in the biomedical field [ 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 ]. In silk fibroin/hyaluronic acid scaffolds, increasing hyaluronic acid content significantly enhanced the water binding capacity and flexibility [ 140 ].…”

Section: Blendingmentioning

confidence: 99%

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Grabska-Zielińska

Sionkowska

2021

Materials

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This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

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Section: Blendingmentioning

confidence: 99%

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Grabska-Zielińska

Sionkowska

2021

Materials

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“…The human umbilical cord mesenchymal stem cells (HUMSCs) were used as the original cells for iPSC generation because they could be easily harvested from clinical wastes after child delivery. , Compared with other cell sources, collecting HUMSCs is a process of waste recycling. As a type of multipotent stromal cell, HUMSCs enjoy the capability of differentiation into a large number cell types, including fat and cartilage cells, , but they lack the pluripotency of differentiation into all three germ layers as ESCs and iPSCs do. Considering their easy accessibility and self-renewable property, HUMSCs would be desirable parental cells for iPSC generation.…”

Section: Introductionmentioning

confidence: 99%

Prolonged Three-Dimensional Co-Delivery of Yamanaka Factors for Cell Reprogramming

Deng

Cao

Wang

et al. 2016

ACS Appl. Mater. Interfaces

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Reprogramming somatic cells into a pluripotent state has been widely investigated in two-dimensional (2D) systems but not described in the more biologically faithful three-dimensional (3D) scaffolds. Here, we devise a 3D porous tissue engineering scaffold that could achieve successful and efficient induction of pluripotency. To construct this 3D scaffold, nonviral hybrid nanoparticles were fabricated beforehand by employing calcium phosphate and cationized Pleurotus eryngii polysaccharide to codeliver plasmids OCT4, SOX2, KLF4 ,and C-MYC (pOSKM). These hybrid nanoparticles were then loaded into a 3D porous collagen scaffold to obtain the so-called pOSKM-activated 3D scaffold. This 3D scaffold could reprogram human umbilical cord mesenchymal stem cells (HUMSCs) into a pluripotent state, generating 3D cell spheres which showed positive expression of pluripotency markers in the 3D scaffolds and tightly packed colonies when transferred to 2D feeder layers. Besides sharing similar morphology, epigenetic modification, and expression of pluripotency genes with the embryonic stem cells, the 3D system-generated colonies could also be expanded on feeder layers for more than 20 passages, indicating the successful establishment of stable induced pluripotent stem cell (iPSC) lines. Our findings represent a first employment of porous 3D scaffolds to achieve successful reprogramming via a one-time transfection, offering a safe, simple, and effective alternative strategy for iPSC generation.

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Chondrogenesis of human umbilical cord-derived mesenchymal stem cells in silk-fibroin/hyaluronic acid scaffolds for cartilage tissue engineering

Cited by 2 publications

References 0 publications

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Prolonged Three-Dimensional Co-Delivery of Yamanaka Factors for Cell Reprogramming

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