Chondrogeneic Potential of MSC from Different Sources in Spheroid Culture

Цветкова, А. В.; Вахрушев, И. В.; Basok, Yu. B.; Григорьев, А. М.; Кирсанова, Л. А.; Lupatov, A. Yu.; Sevastianov, V. I.; Yarygin, K N

doi:10.1007/s10517-021-05101-x

Cited by 31 publications

(24 citation statements)

References 25 publications

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“…The immunophenotypic expression profile of cell markers isolated from the adipose tissue met the criteria of The International Society for Cellular Therapy and confirmed that these cells are multipotent MSCs [ 14 ]. In earlier studies, we found a high level of expression of CD29, CD44, CD49b, CD73, and CD90 in primary culture [ 15 ]. At the same time the expression of CD34, CD45, or HLA-DR was not observed in the culture [ 15 ].…”

Section: Methodsmentioning

confidence: 97%

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A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

Sevastianov

Basok

Кирсанова

et al. 2021

Life

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Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into chondrocytes in vitro and to stimulate the regeneration of articular cartilage in an experimental model of rabbit knee osteoarthrosis when cultured on microheterogenic collagen-based hydrogel (MCH) and the microparticles of decellularized porcine articular cartilage (DPC). The morphology of samples was evaluated using scanning electron microscopy and histological staining methods. On the surface of the DPC, the cells were distributed more uniformly than on the MCH surface. On day 28, the cells cultured on the DPC produced glycosaminoglycans more intensely compared to the MCH with the synthesis of collagen type II. However, in the experimental model of osteoarthrosis, the stimulation of the cartilage regeneration was more effective when the MSCs were administered to the MCH carrier. The present study demonstrates the way to regulate the action of the MSCs in the area of cartilage regeneration: the MCH is more conducive to stimulating cartilage repair by the MSCs, while the DPC is an inducer for a formation of a cartilage-like tissue by the MSCs in vitro.

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Section: Methodsmentioning

confidence: 97%

“…In earlier studies, we found a high level of expression of CD29, CD44, CD49b, CD73, and CD90 in primary culture [ 15 ]. At the same time the expression of CD34, CD45, or HLA-DR was not observed in the culture [ 15 ].…”

Section: Methodsmentioning

confidence: 97%

A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

Sevastianov

Basok

Кирсанова

et al. 2021

Life

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“…The International Society for Cell and Gene Therapy (ISCT ® ) has recently suggested that this term is inappropriate unless the data support their "stem-like" functionality and properties [79]. They are found in the bone marrow (in low amounts: 0.001%), fat tissue (a rich source), umbilical blood and cord, and dental pulp [80][81][82]. The source of these cells should also be indicated and emphasised, because they exhibit different immunomodulatory and paracrine properties [82].…”

Section: Comparison Of Ipsc and Mscmentioning

confidence: 99%

“…However, their protective properties were maintained at a similar level [84]. Some studies have also tested the utility of MSC derived from different sources in cartilage regeneration [81,85,86]. Among them, the best choice for joint regeneration purposes would be the MSC derived from bone marrow [81,85,86].…”

Section: Comparison Of Ipsc and Mscmentioning

confidence: 99%

“…Some studies have also tested the utility of MSC derived from different sources in cartilage regeneration [81,85,86]. Among them, the best choice for joint regeneration purposes would be the MSC derived from bone marrow [81,85,86]. Despite this, their unique features make them an attractive source of cells for a wide range of medical applications.…”

Section: Comparison Of Ipsc and Mscmentioning

confidence: 99%

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The Induced Pluripotent Stem Cells in Articular Cartilage Regeneration and Disease Modelling: Are We Ready for Their Clinical Use?

Lach

Rosochowicz

Richter

et al. 2022

Cells

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The development of induced pluripotent stem cells has brought unlimited possibilities to the field of regenerative medicine. This could be ideal for treating osteoarthritis and other skeletal diseases, because the current procedures tend to be short-term solutions. The usage of induced pluripotent stem cells in the cell-based regeneration of cartilage damages could replace or improve on the current techniques. The patient’s specific non-invasive collection of tissue for reprogramming purposes could also create a platform for drug screening and disease modelling for an overview of distinct skeletal abnormalities. In this review, we seek to summarise the latest achievements in the chondrogenic differentiation of pluripotent stem cells for regenerative purposes and disease modelling.

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Unfolding the Mystery Behind the Onset of Chondrocyte Hypertrophy during Chondrogenesis: Toward Designing Advanced Permanent Cartilage‐mimetic Biomaterials

Majumder

Ghosh

2023

Adv Funct Materials

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Successful recapitulation of the anatomical microarchitecture and biomechanics of the native articular cartilage under in vitro culture conditions is still an elusive topic of research. The major roadblock lies in maintaining the stable chondrogenic phenotype in vivo or under long‐term in vitro conditions. Tissue engineers worldwide has coined this aberrant loss of permanent cartilage characteristics to transient cartilage form as “chondrocyte hypertrophy”. Although the following has been validated through the expression of a few known markers but very little is understood regarding the molecular mechanism that dwells underneath. This review summarizes the precise aetiology behind the development and progression of the hypertrophic phenotype in chondrocytes under in vitro chondrogenic conditions. Based on the current literature survey, it is deciphered that the type of cell utilized (chondrocytes or stem cells), the chondrogenic culture conditions (growth factors/biochemical mediators) and the culture microenvironment (oxygen tension, mechanical loading) during chondrogenesis have a direct correlation with the dysregulated activity of the chondrogenic signaling pathways corroborating the onset of hypertrophic maturation of chondrocytes. Furthermore, it is critically analyzed whether to completely inhibit these hypertrophy‐inducing signaling pathways or apply a brake in terms of time‐dependent dose due to their functional duality role in chondrogenesis.

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Chondrogeneic Potential of MSC from Different Sources in Spheroid Culture

Cited by 31 publications

References 25 publications

A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

The Induced Pluripotent Stem Cells in Articular Cartilage Regeneration and Disease Modelling: Are We Ready for Their Clinical Use?

Unfolding the Mystery Behind the Onset of Chondrocyte Hypertrophy during Chondrogenesis: Toward Designing Advanced Permanent Cartilage‐mimetic Biomaterials

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