Cholinesterase inhibitors isolated from bilberry fruit

Borowiec, Kamila; Szwajgier, Dominik; Targoński, Zdzisław; Demchuk, Oleg M.; Cybulska, Justyna; Czernecki, Tomasz; Malik, Agnieszka

doi:10.1016/j.jff.2014.10.008

Cited by 15 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the extracts used in the present study did no show any activity against acetylcholinesterase, while Lbb and Lbe had a weak and Lbn a moderate inhibitory effect on butyrylcholinesterase. In fact, when comparing the results from the present study with other researchers’ results it seems that not always edible plants rich in phenolic compounds possess potent inhibitory effects on cholinesterase 76 , 77 .…”

Section: Resultssupporting

confidence: 70%

Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies

Mocan

Zengin

Simirgiotis

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

160

114

View full text Add to dashboard Cite

Goji (Lycium barbarum L.) leaves are emphasized as a functional tea or as dietary supplements. The phenolic compound profile, antioxidant, enzyme inhibitory, antimicrobial, and antimutagenic activities of leaf extracts from two selected cultivars in comparison with wild-growing plants have been evaluated. HPLC-DAD/ESI-ToF-MS analysis revealed the presence of phenolic acids and flavonoids with chlorogenic acid and rutin being the dominant compounds in the cultivated plants, whereas rutin and kaempeferol-3-O-rutinoside for wild growing ones. In particular, cv. Erma contained the highest amount of chlorogenic acid and showed a strong tyrosinase-inhibitory effect. Staphylococcus aureus, Listeria monocytogenes, and Penicillium funiculosum were the most sensitive strains when exposed to extracts from cultivated plants. Antimutagenic activity was evaluated by Ames' test. The tested extracts provided high protection against mutagenicity induced by 2-anthramine (2-AA) to Salmonella typhimurium strains TA 98 and TA 100 (max. inhibition (%) 88% and 74.2%, respectively). Overall, Goji leaves are a rich source of bioactive compounds with functional properties that need further risk/benefit evaluation when used in foods or health-promoting formulations.

show abstract

Section: Resultssupporting

confidence: 70%

Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies

Mocan

Zengin

Simirgiotis

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

160

114

View full text Add to dashboard Cite

show abstract

“…Preparation of fruit extract and the next stages of isolation and purification of ChE inhibitors from P. persica fruit were performed according to the previously proposed procedure [10].…”

Section: Preparation Of Freeze-dried Water Extractmentioning

confidence: 99%

Activity-guided isolation of cholinesterase inhibitors quercetin, rutin and kaempferol from Prunus persica fruit

Szwajgier

Borowiec

Zapp

2020

Zeitschrift Für Naturforschung C

Self Cite

View full text Add to dashboard Cite

Prunus persica fruit is a source of anti-cholinesterase agents and led to an increase of acetylcholine levels in brain tissue as a usefull tool in the Alzheimer’s disease therapy. This study aimed to propose a convenient method for the purification of cholinesterase inhibitors from P. persica water extract. Water extract from the fruit was ultrafiltered (0.2 μm→5 kDa→3 kDa) followed by preparative chromatography (Sephadex LH-20, high pressure C18) and high pressure analytical C18 chromatography. The chemical structures of inhibitors were confirmed using infrared and 1H-NMR spectroscopies. The anti-acetylcholinesterase activity was measured using the colorimetric method in fractions obtained after each stage of purification. Polyphenolic cholinesterase inhibitors identified in peach fruit were kaempferol, quercetin and quercetin-3-O-rhamnoglucoside (rutin). The relatively fast purification procedure elaborated in this work can be adopted for the isolation of phenolic cholinesterase inhibitors from fruit extracts related to P. persica fruit.

show abstract

“…Phenolic compounds naturally occurring in food have received much attention as alternative agents for therapy in AD. Anticholinesterase activity has previously been shown in coffee (Erdem et al, 2016), tea (Okello et al, 2012), fruits (Borowiec et al, 2014), vegetables (Nwanna et al, 2016) and juices (Gironés-Vilaplana et al, 2012), but until now, wines have not been pointed out as possible sources of cholinesterase inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Gentisic Acid, Salicylic Acid, Total Phenolic Content and Cholinesterase Inhibitory Activities of Red Wines Made from Various Grape Varieties

Stój

Szwajgier

Baranowska‐Wójcik

et al. 2018

SAJEV

View full text Add to dashboard Cite

Alzheimer's disease is characterised by a decrease in acetylcholine (ACh) levels in the brain due to the activity of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). As a result, disorders in cholinergic transmission are observed, leading to cognitive impairment. In this work, the inhibition of AChE and BChE activities by red wines made of various grape varieties was determined for the first time. There was no significant difference in the polyphenol content between the grape varieties; nevertheless, there were significant differences in the content of gentisic acid and salicylic acid, and in the inhibition of AChE and BChE between the wine samples. A statistically significant correlation between AChE inhibitory activity and salicylic acid, as well as between BChE inhibitory activity and total phenolic content, was recorded. In model solution studies, it was shown that salicylic acid effectively inhibited BChE activity at concentrations similar to the maximum concentrations found in the test wines. Hierarchical cluster analysis (HCA) revealed that the wines could be divided into three groups. Cabernet Sauvignon and Syrah wines had the highest content of salicylic acid and AChE inhibitory activity, as well as low BChE inhibitory activity. Pinot noir, Tempranillo, Regent and Rondo wines showed the lowest content of salicylic acid and low AChE inhibitory activity. Garnacha tinta, Merlot, Montepulciano and Negroamaro wines had a medium content of salicylic acid, and the highest or medium BChE inhibitory activity. This work is important for both the wine industry and for health protection.

show abstract

Cholinesterase inhibitors isolated from bilberry fruit

Cited by 15 publications

References 36 publications

Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies

Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies

Activity-guided isolation of cholinesterase inhibitors quercetin, rutin and kaempferol from Prunus persica fruit

Gentisic Acid, Salicylic Acid, Total Phenolic Content and Cholinesterase Inhibitory Activities of Red Wines Made from Various Grape Varieties

Contact Info

Product

Resources

About