Cholinesterase in Adult and Embryonic Hypothalamus

Duffy, Philip E.

doi:10.1001/archneur.1967.00470220049006

Cited by 21 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The1~e are data in the literature on the fact that during ontogenesls of immaturely born mammals, the total brain AChE activity increases from the moment of birth up to the 22nd day of llfe [2][3][4][5][6][7][8]. At the same time, in maturely born animals the brain AChE activity of the newborn is approximately the same as in adults.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

External acetylcholinesterase of the brain in the ontogenesis of maturely and immaturely born rodents

Маслова

Ozirskaya

1981

Neurosci Behav Physiol

View full text Add to dashboard Cite

The fraction of external acetylcholinesterase (AChE) in various divisions of the brain was studied in maturely and immaturely born rodents. It was established that in maturely born rodents (guinea pigs) the fraction of external AChE in all the investigated division of the brain is the same by the time of birth of the animals as in the adults. In immaturely born rodents (white rats), by the moment of birth the fraction of external AChE is significantly higher than in adults. As the brain matures there is a decrease in the proportion of external AChE, most pronounced in the forebrain. It is suggested and experimentally confirmed (in experiments with detergents) that the decrease in the fraction of external AChE is associated with a strengthening of the fixation of the enzyme in the membrane.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Homogenates and protein extracts of the cerebral hemispheres, necessary for electrophoresis, were produced according to the scheme described earlier [i]. A determination of the AChE activity in a brain homogenate was conducted according to the Hestrin method [3]. Acetyl-B-methylcholine bromide was used as the substrate.…”

Section: ~Terials and Methodsmentioning

confidence: 99%

External acetylcholinesterase of the brain in the ontogenesis of maturely and immaturely born rodents

Маслова

Ozirskaya

1981

Neurosci Behav Physiol

View full text Add to dashboard Cite

show abstract

“…3 It is important to recognize that cancer treatments have improved over this time with the advent of chemotherapy, lower toxicity treatment regimens, and more focused radiation therapy. Anecdotal reports have documented late effects of cranial radiation decades after therapy.…”

Section: Pediatrics and Internal Medicine University Of Rochester Mementioning

confidence: 99%

Promoting Breastfeeding: What Works and What Doesn’t

Va¹

2003

AAP Grand Rounds

View full text Add to dashboard Cite

Acetylcholinesterase and choline acetyltransferase activity in the developing chick spinal cord

Burt

1968

J. Exp. Zool.

View full text Add to dashboard Cite

Microchemical procedures were employed for the quantitative assay of acetylcholinesterase ( AChE) and choline acetyltransferase (ChAc) activity in the brachial spinal cord of chick embryos from 3.5 to 21 days' incubation. The activity of AChE increased nearly 10-fold during the 60-hour period from stage 22 (3.5 days) to stage 30 (6.0 days). The level of AChE at six days was essentially the same as that at hatching. During the intervening period, the level of AChE activity remained unchanged with the exception of minor transient peaks a t days 7, 13, and 18. The activity of ChAc increased 22-fold during the developmental period studied. The ontogenetic pattern was characterized by two periods of rapid increase in acitvity. From stage 23 (3.75 days) to stage 31 (7.0 days), ChAc activity increased approximately 5.4-fold and from stage 38+ (12.5 days) to stage 44 (18 days) ChAc activity increased approximately 3.6-fold with the major increase occurring during the 48-hour period from stage 42 to stage 44. The ontogenetic patterns for both enzymes are discussed in relation to morphological and functional differentiation. It is suggested that ChAc activity is a more reliable index of functional differentiation than is AChE and that the initial increase in AChE activity may be associated with some function other than synaptic transmission.The enzymes associated with cholinergic transmission, AChE and ChAc, increase in specific activity during the development of the central nervous system in amphibians (Sawyer, '43; Boell and Shen, '50), birds (Nachmansohn, '39; Wenger, '51; Rogers et al., '60; Burdick and Strittmatter, '65; Turbow and Burkhalter, '68), and mammals (Nachmansohn, '40; Kavaler and Kimel, '52; Hebb, '56; McCaman and Aprison, '64; Maletta and Timiras, '66; Maletta et al., '67; Duffy et al., '67; Tennyson and Brzin, '68).Since the classical studies of Nachmansohn ('39,'40) and Sawyer ('43), numerous investigations have associated the increased AChE activity with the onset of neural function during embryogenesis. However, Hebb ('56), after considering the distribution of acetylcholine, AChE and ChAc in the adult central nervous system, suggested that ChAc activity might be a more reliable index of cholinergic function. Until the development of a sensitive assay procedure for ChAc activity (McCaman and Hunt, '65), few embryological studies included both enzymes.In this study microchemical procedures were employed for the assay of both AChE J. EXP. Zoos., 169: 107-112.and ChAc activity in the brachial spinal cord of the chick embryo. The specific activities of both enzymes increased during the developmental period studied; however, the ontogenetic patterns were not similar. The results of this study, when related to recent electron microscopic and physiological studies, suggest that increased ChAc activity may be a reflection of functional differentiation whereas increased AChE activity initially may reflect the growth and differentiation of the neuroblast and its processes rather than the onset of n...

show abstract

Cholinesterase in Adult and Embryonic Hypothalamus

Cited by 21 publications

References 34 publications

External acetylcholinesterase of the brain in the ontogenesis of maturely and immaturely born rodents

External acetylcholinesterase of the brain in the ontogenesis of maturely and immaturely born rodents

Promoting Breastfeeding: What Works and What Doesn’t

Acetylcholinesterase and choline acetyltransferase activity in the developing chick spinal cord

Contact Info

Product

Resources

About