Cholinergic systems of the rat brain and neuronal reorganization under conditions of acute hypoxia

Захарова, Е. И.; Dudchenko, A. M.; Svinov, M. M.; Федорова, Мария Михайловна; Germanova, E. L.

doi:10.1134/s1819712410040082

Cited by 7 publications

(17 citation statements)

References 50 publications

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“…It is necessary to understand the true mechanisms of the regulation, maintenance and recovery of brain functions. It should be noted that studies on synaptic fraction levels were carried out along these lines from the beginning [4,18,[21][22][23][24][25][26][27][28][29][30][31].…”

Section: Natural Markers Of the Neuronal Systemsmentioning

confidence: 99%

“…cChAT activation was observed under acute ischemia or hypoxia at all intensities [27,29,141]. mChAT or both mChAT and cChAT activation was revealed under acute and chronic ischemia and only in severe hypoxia (4.5% O 2 ) [27][28][29]141]. When the activation of ChAT was observed (165-170%), extracellular mAChE (the predominant isoform of mAChE) was simultaneously activated [141].…”

Section: Biochemical Equivalents Of Activation and Inhibition Of Cholmentioning

confidence: 99%

“…When the activation of ChAT was observed (165-170%), extracellular mAChE (the predominant isoform of mAChE) was simultaneously activated [141]. cChAT activation positively correlated with the activation of Na + /K + -ATPase and negatively correlated with the decrease in the c-protein content [27]. All these reactions of the synaptic biochemical parameters and their combinations are regarded as the activation of cholinergic synaptic function, because they conform to the characteristics of synaptic activation.…”

Section: Biochemical Equivalents Of Activation and Inhibition Of Cholmentioning

confidence: 99%

“…Compared to cChAT, the selective activation of mChAT has been revealed (1) under equal experimental conditions (3 hours of ischemia) in rats less resistant to hypoxia [141], and (2) under hypoxic conditions with variable intensity only in severe hypoxia [27] and was not observed in the subcritical and moderate hypoxia (6.5% or 10% O 2 ) [27,29]. A parallel study of the ultrastructure of the synapses in the cortex revealed the dependence of swelling synapses and synaptic mitochondria on the duration of severe hypoxia [27]. Taken together, these data suggest that the activation of mChAT in vivo occurs due to an imbalance of synaptic Ca2 + , while cChAT activation is apparently initiated in the natural physiological way, under neuronal influences [27].…”

Section: Biochemical Equivalents Of Activation and Inhibition Of Cholmentioning

confidence: 99%

See 3 more Smart Citations

Synaptic Soluble and Membrane-Bound Choline Acetyltransferase as a Marker of Cholinergic Function In Vitro and In Vivo

Захарова¹,

Dudchenko²

2014

Neurochemistry

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Section: Natural Markers Of the Neuronal Systemsmentioning

confidence: 99%

Section: Biochemical Equivalents Of Activation and Inhibition Of Cholmentioning

confidence: 99%

Section: Biochemical Equivalents Of Activation and Inhibition Of Cholmentioning

confidence: 99%

Section: Biochemical Equivalents Of Activation and Inhibition Of Cholmentioning

confidence: 99%

See 2 more Smart Citations

Synaptic Soluble and Membrane-Bound Choline Acetyltransferase as a Marker of Cholinergic Function In Vitro and In Vivo

Захарова¹,

Dudchenko²

2014

Neurochemistry

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“…ПК -поясная кора, Пер -перегородка, ЯЛТП -ядро ложа терминальной полоски цессов обучения, памяти, сна. Они также обеспечивают двигательные функции и участвуют в регулировании моторики желудочно-кишечного тракта [32][33][34][35][36].…”

Section: Immunohistochemical Markers For Neurobiologyunclassified

Immunohistochemical markers for neurobiology

Коржевский

Эдуардович²,

Grigor’ev

et al. 2020

Medical academic journal

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In neurobiological studies, crucial is the selection of most appropriate and informative experimental methods, one of which is immunohistochemistry. This review briefly summarizes the experience of adaptation of immunohistochemical methods to nervous system studies accumulated over years the Laboratory of Functional Morphology of the Central and Peripheral Nervous System (Institute of Experimental Medicine). The aim of this work was to determine the most effective and reliable immunomarkers for neurobiological studies. The article contains theoretical basis and practical recommendations for use of key cytospecific and functional markers used in studies of structural and functional organization of brain and spinal cord of mammalian animals and human. In particular, the results of immunohistochemical reactions to neural markers (NeuN, neurofilament proteins, alpha-tubulin, alpha-synuclein), neurotransmitter synthesizing enzymes (tyrosine hydroxylase, glutamate decarboxylase, choline acetyltransferase, NO synthase) and glial markers (GFAP, glutamine synthetase, Iba-1, vimentin) are demonstrated. The presented methodology is useful for experimental neurobiology and clinical morphological diagnostics.

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Central cholinergic systems in the mechanisms of hypoxic preconditioning: Diverse pathways of synaptic reorganization in vivo

et al. 2013

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Cholinergic systems of the rat brain and neuronal reorganization under conditions of acute hypoxia

Cited by 7 publications

References 50 publications

Synaptic Soluble and Membrane-Bound Choline Acetyltransferase as a Marker of Cholinergic Function In Vitro and In Vivo

Synaptic Soluble and Membrane-Bound Choline Acetyltransferase as a Marker of Cholinergic Function In Vitro and In Vivo

Immunohistochemical markers for neurobiology

Central cholinergic systems in the mechanisms of hypoxic preconditioning: Diverse pathways of synaptic reorganization in vivo

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