“…Lastly, although it is tempting to assert that the modulation of Dα1 and Dα6 expression results solely from transcriptional control based on the clear developmental shifts in their transcript levels, our work did not rule out the involvement of posttranslational regulatory steps, which may influence the folding, processing, and synaptic integration of nAchR subunits during cholinergic synapse development. A number of nAchR-associated accessory proteins, such as RIC-3, 14-3-3 proteins, LRP4, and Nacho, have been identified in vertebrate, Caenorhabditis elegans, and/or Drosophila systems (21,45,46,(49)(50)(51)(52) and deserve attention in future work for roles they play in ensuring the correct expression and function of Dα1-and Dα6-containing receptor subtypes.…”