Cholinergic receptor pathways involved in apoptosis, cell proliferation and neuronal differentiation

Resende, Rodrigo R.; Adhikari, Avishek

doi:10.1186/1478-811x-7-20

Cited by 154 publications

(101 citation statements)

References 267 publications

(335 reference statements)

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“…Bone regeneration, fracture healing, and more recently stem cell differentiation could be directed with the use of an electric field. [130][131][132][133] The results of unstimulated osteoblast assay showed that the aligned nanofibers as topographic cues could enhance the extension and direct the outgrowth of osteoblasts better than random fibers. In the presence of direct current, the osteoblasts on all samples grew along the electrical current direction.…”

Section: -105mentioning

confidence: 99%

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Tonelli¹,

Santos²,

Gomes³

et al. 2012

IJN

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Abstract:In recent years, significant progress has been made in organ transplantation, surgical reconstruction, and the use of artificial prostheses to treat the loss or failure of an organ or bone tissue. In recent years, considerable attention has been given to carbon nanotubes and collagen composite materials and their applications in the field of tissue engineering due to their minimal foreign-body reactions, an intrinsic antibacterial nature, biocompatibility, biodegradability, and the ability to be molded into various geometries and forms such as porous structures, suitable for cell ingrowth, proliferation, and differentiation. Recently, grafted collagen and some other natural and synthetic polymers with carbon nanotubes have been incorporated to increase the mechanical strength of these composites. Carbon nanotube composites are thus emerging as potential materials for artificial bone and bone regeneration in tissue engineering.

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Section: -105mentioning

confidence: 99%

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Tonelli¹,

Santos²,

Gomes³

et al. 2012

IJN

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“…ChAT is produced in the body of neurons and is transported to the nerve terminal [13]. Among its other functions, acetylcholine is pivotal in the development and maintenance of memory and cognitive function in the brain [14]. Thus, this suggest that dIMR32 cells can serve an in vitro model to study neuroprotective effect of plant bioactives that affects the memory function in the brain.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

et al. 2017

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Background: Peltophorum pterocarpum is a plant from the family of Leguminosae and a tropical tree found in South East Asia region. The leaves of the plant is rich in anti-oxidant polyphenols. Traditionally this plant was used to cure intestinal disorders and as a relief for sprain, bruise, muscular pain and sores and for pain during child birth. Since oxidative stress is a major contributing factor towards neurodegenerative diseases the leaves of this plant was explored for its neuroprotective properties. Method: In this study, we report the neuroprotective properties of the ethanolic extract of Peltophorum pterocarpum leaf against oxidative stress induced cell death by H 2 O 2 , in an in vitro model of neuronally differentiated IMR32 neuroblastoma cells. Hydrogen peroxide was used as an inducer for oxidative stress. Cell viability was determined using MTT assay, apoptosis studies were carried out using Annexin V-FITC and Caspase 3 assays, mitochondrial membrane potential was examined using JC1 staining. The oxidative stress induced by H 2 O 2 was determined by quantifying the intracellular reactive oxygen species production using DCF-DA staining. Mitogen activated protein kinase signaling pathway of the neuroprotective effect of PTE was also elucidated since oxidative stress activates this pathway signaling.

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“…The activation of ERK1/2 has been shown to lead to cellular proliferation, whereas JNK and p38 MAPK activation is associated with inflammatory cytokine action, cellular stress and apoptosis [28,29]. MAPK/ERK1/2 can be activated through PKC-dependent mechanism [30]. To clarify the mechanism by which ketamine exerts its inhibitory effect on NSC proliferation, we observed the effect of ketamine on PKCα and ERK1/2 activation.…”

Section: Discussionmentioning

confidence: 99%

Ketamine Inhibits Proliferation of Neural Stem Cell from Neonatal Rat Hippocampusin Vitro

Wu¹,

Liang²,

Huang³

et al. 2014

Cell Physiol Biochem

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Background/Aims: Ketamine is a widely used anesthetic in obstetric and pediatric anesthesia. In the developing brain, the widespread neuron apoptosis triggered by ketamine has been demonstrated. However, little is known about its effect on neural stem cells (NSCs) function. This study aimed to investigate the effect of ketamine on proliferation of NSCs from neonatal rat hippocampus. Methods: Neural stem cells were isolated from the hippocampus of Sprague-Dawley rats on postnatal day 3. In dose-response experiments, cultured neural stem cells (NSCs) were exposed to different concentrations of ketamine (0-1000 µM) for 24 hrs. The proliferative activity of NSCs was evaluated by 5-Bromo-2′-deoxyuridine (BrdU) incorporation assay. Apoptosis of neural stem cells were assessed using caspase-3 by western blot. The intracellular Ca²⁺ concentration ([Ca²⁺]i) in NSCs was analyzed by flow cytometry. The activation of protein kinase C-α (PKCα) and the phosphorylation of extracellular signal-regulated kinases 1/2 (ERK1/2) were measured by western blot analysis. Results: Clinical relevant concentration of ketamine (10, 20 and 50 µM) did not markedly alter the proliferation of NSCs from neonatal rat hippocampus in vitro. However, ketamine (200, 500, 800 and 1000μM) significantly inhibited the proliferation of NSCs and did not affect the expression of caspase-3. Meanwhile, ketamine (200, 500, 800 and 1000μM) also markedly decreased [Ca²⁺]i as well as suppressed PKCα activation and ERK1/2 phosphorylation in NSCs. A combination of subthreshold concentrations of ketamine (100 μM) and Ca²⁺ channel blocker verapamil (2.5 μM), PKCα inhibitor chelerythrine (2.5 μM) or ERK1/2 kinase inhibitor PD98059 (5 μM) significantly produced suprathreshold effects on PKCα activation, ERK1/2 phosphorylation and NSC proliferation. Conclusion: Ketamine inhibited proliferation of NSCs from neonatal rat hippocampus in vitro. Suppressing Ca²⁺-PKCα-ERK1/2 signaling pathway may be involved in this inhibitory effect of ketamine on NSCs proliferation.

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Cholinergic receptor pathways involved in apoptosis, cell proliferation and neuronal differentiation

Cited by 154 publications

References 267 publications

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

Ketamine Inhibits Proliferation of Neural Stem Cell from Neonatal Rat Hippocampusin Vitro

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