Choline transporter‐like1 (<scp>CHER</scp>1) is crucial for plasmodesmata maturation in <i>Arabidopsis thaliana</i>

Kraner, Max; Link, Katrin; Melzer, Michael; Ekici, Arif B.; Uebe, Steffen; Tarazona, Pablo; Feußner, Ivo; Hofmann, Jörg; Sonnewald, Uwe

doi:10.1111/tpj.13393

Cited by 38 publications

(49 citation statements)

References 58 publications

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“…Furthermore, recent work has demonstrated that impairing choline transport, by CHER1 choline transporter inactivation, has an impact on the development of the root vasculature and plasmodesmata in particular (Dettmer et al, 2014;Kraner et al, 2017). In light of the absence of strong architectural alterations and lipid modifications caused by the greatly diminished PCho concentration in our plant lines, the CHER1 mutant phenotype is more likely linked to choline or PtdCho imbalance rather than to lower PCho content (Kraner et al, 2017).…”

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 79%

“…In light of the absence of strong architectural alterations and lipid modifications caused by the greatly diminished PCho concentration in our plant lines, the CHER1 mutant phenotype is more likely linked to choline or PtdCho imbalance rather than to lower PCho content (Kraner et al, 2017 content and lipid composition. Future research will focus on verifying how these alterations can preserve the lipid composition in membranes while ensuring their effective recycling in Pi-starved conditions.…”

Section: Discussionmentioning

confidence: 79%

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The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

et al. 2018

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Phosphate starvation-mediated induction of the HAD-type phosphatases PPsPase1 (AT1G73010) and PECP1 (AT1G17710) has been reported in Arabidopsis (Arabidopsis thaliana). However, little is known about their in vivo function or impact on plant responses to nutrient deficiency. The preferences of PPsPase1 and PECP1 for different substrates have been studied in vitro but require confirmation in planta. Here, we examined the in vivo function of both enzymes using a reverse genetics approach. We demonstrated that PPsPase1 and PECP1 affect plant phosphocholine and phosphoethanolamine content, but not the pyrophosphate-related phenotypes. These observations suggest that the enzymes play a similar role in planta related to the recycling of polar heads from membrane lipids that is triggered during phosphate starvation. Altering the expression of the genes encoding these enzymes had no effect on lipid composition, possibly due to compensation by other lipid recycling pathways triggered during phosphate starvation. Furthermore, our results indicated that PPsPase1 and PECP1 do not influence phosphate homeostasis, since the inactivation of these genes had no effect on phosphate content or on the induction of molecular markers related to phosphate starvation. A combination of transcriptomics and imaging analyses revealed that PPsPase1 and PECP1 display a highly dynamic expression pattern that closely mirrors the phosphate status. This temporal dynamism, combined with the wide range of induction levels, broad expression, and lack of a direct effect on Pi content and regulation, makes PPsPase1 and PECP1 useful molecular markers of the phosphate starvation response.Plant growth is highly sensitive to a lack of phosphate (Pi); hence, the application of Pi fertilizers has become standard practice for high-throughput crop production (Cordell et al., 2009). Most phosphorus in the soil is not available to plants, as it is combined with other minerals or parts of organic compounds (Bieleski, 1973;Raghothama and Karthikeyan, 2005). Only a small fraction of soluble Pi (usually present at a concentration of ,10 mM in the soil) can be taken up by plants.Although growth is not optimal under limiting conditions, plants can withstand changing Pi concentrations within heterogeneous soils or in the external nutrient supply that can lead to reprogramming of their metabolism and architecture (Hammond et al., 2003;Péret et al., 2011;Plaxton and Tran, 2011). Root architecture can be modified to facilitate Pi uptake by favoring the development of lateral roots (at the expense of primary root elongation in many plants including Arabidopsis), increasing the density and length of root hairs, and limiting the development of aerial parts (López-Bucio et al., 2002;Svistoonoff et al., 2007;Gruber et al., 2013). Pi uptake mechanisms are enhanced at the root/soil interface, particularly through the stimulation of Pi transport activity (Mudge et al., 2002;Shin et al., 2004;Nussaume et al., 2011; Ayadi et al., 2015). In parallel, mobilization of Pi sources fr...

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 79%

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The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

et al. 2018

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“…and Cho (Jost et al, 2009). NMT1 expression is known to be sensitive to feedback inhibition leaves (Dettmer et al, 2014;Kraner et al, 2017). Choline has also long been known to be 582 present in xylem sap (Maizel et al, 1956;Martin et al, 1983).…”

mentioning

confidence: 99%

NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction

et al. 2018

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54Phosphatidylcholine (PC) is a major membrane phospholipid and a precursor for most 55 signalling molecules. Understanding its synthesis is important for improving plant growth, 56 nutritional value and resistance to stress. PC synthesis is complex, involving several 57 interconnected pathways, one of which proceeds from serine-derived phosphoethanolamine 58(PEA) to form phosphocholine (PCho) through three sequential phospho-base methylations 59 catalysed by phosphoethanolamine N-methyltransferases (PEAMTs). The contribution of 60 this pathway to the production of PC and plant growth has been a matter of some debate.

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“…The molecular mechanisms and role of such dynamic modification of PD frequency are unknown. Recently, however, it has been reported that a choline transporter is involved in the insertion of secondary PD during Arabidopsis development [77]. Mutants in CHOLINE TRANSPORTER-LIKE 1 ( CHER1 ), which encodes a choline transporter, have altered the localization of a plant virus movement protein at PD.…”

Section: Regulatory Mechanisms Of Pd Movementmentioning

confidence: 99%

Plasmodesmata-Mediated Cell-to-Cell Communication in the Shoot Apical Meristem: How Stem Cells Talk

Kitagawa

Jackson

2017

Plants

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Positional information is crucial for the determination of plant cell fates, and it is established based on coordinated cell-to-cell communication, which in turn is essential for plant growth and development. Plants have evolved a unique communication pathway, with tiny channels called plasmodesmata (PD) spanning the cell wall. PD interconnect most cells in the plant and generate a cytoplasmic continuum, to mediate short- and long-distance trafficking of various molecules. Cell-to-cell communication through PD plays a role in transmitting positional signals, however, the regulatory mechanisms of PD-mediated trafficking are still largely unknown. The induction and maintenance of stem cells in the shoot apical meristem (SAM) depends on PD-mediated cell-to-cell communication, hence, it is an optimal model for dissecting the regulatory mechanisms of PD-mediated cell-to-cell communication and its function in specifying cell fates. In this review, we summarize recent knowledge of PD-mediated cell-to-cell communication in the SAM, and discuss mechanisms underlying molecular trafficking through PD and its role in plant development.

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Choline transporter‐like1 (CHER1) is crucial for plasmodesmata maturation in Arabidopsis thaliana

Cited by 38 publications

References 58 publications

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction

Plasmodesmata-Mediated Cell-to-Cell Communication in the Shoot Apical Meristem: How Stem Cells Talk

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