Choline stimulates synthesis of extracellular proteins in Trichoderma reesei QM 9414

Schreiber, Gerhard; Kolar, H.; Foisner, Roland; Kubicek, Christian P.

doi:10.1007/bf00454954

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…As for the first, Schreiber et al [32] showed that the addition of the phospolipid precursor cholin increased cellulase formation in T. reesei , and at the same time led to an increase in the hyphal content of endoplasmic reticulum. Also, Glenn et al [33] showed that the hypercellulolytic mutant T. reesei RUT C30 exhibits a proliferated amount of endoplasmic reticulum.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of the Trichoderma reeseitranscriptome during growth on the cellulase inducing substrates wheat straw and lactose

Bischof

Fourtis

Limbeck

et al. 2013

Biotechnol Biofuels

109

105

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BackgroundRenewable lignocellulosic biomass is an advantageous resource for the production of second generation biofuels and other biorefinery products. In Middle Europe, wheat straw is one of the most abundant low-cost sources of lignocellulosic biomass. For its efficient use, an efficient mix of cellulases and hemicellulases is required. In this paper, we investigated how cellulase production by T. reesei on wheat straw compares to that on lactose, the only soluble and also cheap inducing carbon source for enzyme production.ResultsWe have examined and compared the transcriptome of T. reesei growing on wheat straw and lactose as carbon sources under otherwise similar conditions. Gene expression on wheat straw exceeded that on lactose, and 1619 genes were found to be only induced on wheat straw but not on lactose. They comprised 30% of the CAZome, but were also enriched in genes associated with phospholipid metabolism, DNA synthesis and repair, iron homeostatis and autophagy. Two thirds of the CAZome was expressed both on wheat straw as well as on lactose, but 60% of it at least >2-fold higher on the former. Major wheat straw specific genes comprised xylanases, chitinases and mannosidases. Interestingly, the latter two CAZyme families were significantly higher expressed in a strain in which xyr1 encoding the major regulator of cellulase and hemicellulase biosynthesis is non-functional.ConclusionsOur data reveal several major differences in the transcriptome between wheat straw and lactose which may be related to the higher enzyme formation on the former and their further investigation could lead to the development of methods for increasing enzyme production on lactose.

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Section: Discussionmentioning

confidence: 99%

Comparative analysis of the Trichoderma reeseitranscriptome during growth on the cellulase inducing substrates wheat straw and lactose

Bischof

Fourtis

Limbeck

et al. 2013

Biotechnol Biofuels

109

105

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“…reesei by addition of choline or Tween 80 has been reported recently, using cellulose as a carbon source (Schreiber et al, 1986 ;. However, during growth on this carbon source, mycelia cannot be separated from the insoluble cellulose, and hence preparation of mycelial subcellular fractions is difficult.…”

Section: Stimulation Of T Reesei Cellulase and Protein Secretion Bymentioning

confidence: 99%

“…The mechanism of this stimulation is not clear: in bacteria, these agents appear to cause alterations in membrane fluidity (Umesaki et al, 1977 ;Wittenberger et al, 1978), but this effect has not yet been demonstrated in filamentous fungi. For Trichoderma reesei, a fungus producing high amounts of cellulases (Enari & NikuPaavola, 1987), we have recently shown that addition of phospholipid precursors (Schreiber et al, 1986) or Tween-surfactants has little effect on membrane fluidity, but appears to stimulate formation of intracellular membranous structures. T. reesei exoproteins are usually 0-and N-glycosylated (Merivuori et Abbreoiutions : Dol-P, dolichol phosphate; Dol-P-Man, dolichol phosphate mannose.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of exoprotein secretion by choline and Tween 80 in Trichoderma reesei QM 9414 correlates with increased activities of dolichol phosphate mannose synthase

Kruszewska

Palamarczyk

Kubicek

1990

Journal of General Microbiology

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~Addition of choline (20 mM) or Tween 80 (0.06 %) to the culture medium of Trichoderma reesei QM 9414 increased (a) the secretion of protein under both carbon-catabolite-repressed and -derepressed conditions, and (b) cellulase secretion under carbon-catabolite-derepressed conditions. In contrast, no stimulation by choline or Tween 80 was observed with the hypersecretory strain T. reesei RUT C-30. In view of the obligatory role of 0-glycosylation in protein secretion by this fungus, an investigation was made into the effects on this process of choline and Tween 80.A membrane preparation was isolated from both strains of T. reesei and used to assay enzymes involved in 0-glycosylation. Significant differences were observed with respect to the activity of dolichol phosphate mannose (Dol-P-Man) synthase only. Strain QM 9414, grown on media supplemented with choline or Tween 80 exhibited a two-to threefold higher activity of Dol-P-Man synthase compared to a control lacking these supplements. This stimulatory effect was observed during growth under both carbon-catabolite-repressed and -derepressed conditions. In contrast, strain RUT C-30 exhibited decreased activities of Dol-P-Man synthase when grown in media supplemented with choline. Choline had no effect on Dol-P-Man synthase in vifro, whereas Tween 80 decreased the activity. Thus the effect of Tween 80 or choline on protein secretion by T. reesei may be due to a stimulation of formation and/or activity of Dol-P-Man synthase, thereby elevating the level of 0-glycosylation and protein secretion.

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“…In a series of yeast mutants blocked at various steps, intracellular accumulation of secretory proteins appeared to follow enhancement of ER (Novicla et al 1981). Schreiber et al (1986) reported the effect of choline on ER content: they proposed that its increase in T. reesei QM9414 was directly associated with the hyperproduction of extracellular enzymes. Since ER is involved in extracellular enzyme synthesis, they suggest that protein excretion in T. reesei lower-producer strains is limited at the initial step of their secretory pathway.…”

Section: Discussionmentioning

confidence: 99%

Increased endoplasmic reticulum content of Phanerochaete chrysosporium INA-12 by inositol phospholipid precursor in relation to peroxidase excretion

Moukha

Capdevila

Lesage³

et al. 1991

Appl Microbiol Biotechnol

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Haem protein excretion (i.e., lignin and manganese-dependent peroxidases) by Phanerochaete chrysosporium INA-12 was improved in response to an exogenous supply of phospholipid components (inositol and linoleic acid) as well as phosphatidylinositol (PI). Maximal enzyme productions were 46.3 and 21.1 nkat-ml-1, respectively, in inositol cultures after 3 days incubation. Cellular compartment determination by marker enzymes revealed that the enhancement of protein excretion with inositol was correlated with a proliferation of endoplasmic reticulum; cytosol and mitochondrial activity did not change. In contrast, in the presence of linoleic acid and PI total cellular activity was increased. In culture containing inositol, the intracellular phospholipid composition of strain INA-12 mycelium exhibited a two fold enrichment in PI at the expense of phosphatidylserine and its derives, phosphatidylethanolamine and phosphatidylcholine.

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Choline stimulates synthesis of extracellular proteins in Trichoderma reesei QM 9414

Cited by 7 publications

References 26 publications

Comparative analysis of the Trichoderma reeseitranscriptome during growth on the cellulase inducing substrates wheat straw and lactose

Comparative analysis of the Trichoderma reeseitranscriptome during growth on the cellulase inducing substrates wheat straw and lactose

Stimulation of exoprotein secretion by choline and Tween 80 in Trichoderma reesei QM 9414 correlates with increased activities of dolichol phosphate mannose synthase

Increased endoplasmic reticulum content of Phanerochaete chrysosporium INA-12 by inositol phospholipid precursor in relation to peroxidase excretion

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