Choline-induced selective fluorescence quenching of acetylcholinesterase conjugated Au@BSA clusters

Mathew, Meegle S.; Baksi, Ananya; Pradeep, Thalappil; Joseph, Kuruvilla

doi:10.1016/j.bios.2016.02.048

Cited by 32 publications

(15 citation statements)

References 36 publications

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“…Perception is affected in of derangements such as AD 32 . An unusually low concentration of ACh can be create several neuropsychological and neuropsychiatric perturbations such as AD and Parkinson's disease 33 . Generally, the treatment of AD is centralized on AChE inhibitors, such as rivastigmine, tacrine, galantamine and donepezil 34 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis and bioactivity of several new hetaryl sulfonamides

Taslimi

Sujayev

Mamedova

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

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Abstract1-(4-Methylsulfonyl)-2-thione-4-aryl-5-Z-6-methyl and oxyalkyl-imidazoles were synthesized from different tetrahydropyrimidinethiones and aryl sulfonyl chloride. These compunds were tested for metal chelating effects and to determine the phrase in which inhibition occured between two physiologically pertinent compunds and carbonic anhydrase (CA) isozymes I and II (hCA I and II), butyrylcholinesterase (BChE) and acetylcholinesterase (AChE). AChE was detected in high concentrations in the brain and red blood cells. BChE is another enzymes that is abundant available in the liver and released into the blood in a soluble form. Newly synthesized hetaryl sulfonamides exhibited impressive inhibition profiles with Ki values in the range of 1.42–6.58 nM against hCA I, 1.72–7.41 nM against hCA II, 0.20–1.14 nM against AChE and 1.55–5.92 nM against BChE. Moreover, acetazolamide showed Ki values of 43.69 ± 6.44 nM against hCA I and 31.67 ± 8.39 nM against hCA II. Additionally, tacrine showed Ki values of 25.75 ± 3.39 nM and 37.82 ± 2.08 against AChE and BChE, respectively.

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Section: Introductionmentioning

confidence: 99%

Synthesis and bioactivity of several new hetaryl sulfonamides

Taslimi

Sujayev

Mamedova

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Precise syntheses of the quantum clusters using novel synthetic strategies are an emerging area of recent research. In the past decades, synthesis of gold quantum clusters were achieved using various templates like peptides, amino acids, enzymes, dendrimers, DNA, and proteins. − Among these, protein template-based synthesis of the cluster has received much attention due to its simplicity in preparation and potential biomedical application . The protein protected metal quantum clusters provide more attractive features such as good aqueous solubility, excellent biocompatibility, and versatile surface chemistry .…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Gluten-Stabilized Fluorescent Gold Quantum Clusters: Application As Turn-On Sensing of Human Blood Creatinine

Mathew

Joseph

2017

ACS Sustainable Chem. Eng.

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Protein mediated syntheses of fluorescent noble metal quantum clusters have proven to be particularly attractive owing to their high stability and biocompatibility. Here, we present the cost-effective synthesis of novel, water-soluble, and stable fluorescent gold quantum clusters via a facile and green method using wheat gluten protein as a stabilizing agent (AuQC@gluten). Gluten, a cysteine-rich protein serves as an effective stabilizing agent for these clusters. The AuQC@gluten shows intense red emission at ∼680 nm and is characterized using UV–vis spectroscopy, fluorescence, Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). Contrary to any other protein protected gold quantum cluster, AuQC@gluten is highly stable toward reactive oxygen species like H2O2, revealing its promising application in biomedical fields such as bioimaging, biolabeling, etc. The red emitting AuQC@gluten has been applied in the detection of creatinine with high sensitivity and selectivity. The detection limit is found to be 2 nM in the linear range from 20 μM to 520 μM. This method allows the accurate detection of creatinine in clinical blood samples, indicating its promising biomedical applications.

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“…Further, experiments were extended toward the development of paper test strip. Similar to the BSA stabilized AuNCs, 11,41 the EST-AuNCs were found to be stable at the polymeric support. For this purpose, the small pieces of Whatman paper no.…”

Section: ■ Results and Discussionmentioning

confidence: 75%

Esterase-Mediated Highly Fluorescent Gold Nanoclusters and Their Use in Ultrasensitive Detection of Mercury: Synthetic and Mechanistic Aspects

2018

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The fast, accurate, and ultrasensitive detection of toxic mercury in real water samples is still challenging without the use of expensive sophisticated instruments. Herein, highly fluorescent gold nanoclusters (AuNCs) were synthesized using a newer protein templet, esterase (EST). The EST-AuNCs consisted of ∼25 Au atoms in the nanocluster having ∼2 nm size. EST-AuNCs were found to be highly stable in aqueous buffer with a wide range of pH (pH 4–12) and were also stable in powdered form. The fluorescence quantum yield of EST-AuNCs in deionized water was 6.2% which had increased to 7.8% upon the addition of 1 M NaCl (an increase of 23%). The EST-AuNCs selectively sense the toxic Hg 2+ ions with higher sensitivity (limit of detection; 0.88 nM) with the linear range 1–30 nM. The test strips for rapid sensing of Hg 2+ in real water samples were developed on the polymeric surface. The validation of sensing ability of EST-AuNCs suggested 94–98% recovery with linearity. Moreover, because of the widely reported applications of EST, the developed EST-AuNCs could also be used for another sensing, catalytic, and biomedical applications.

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Choline-induced selective fluorescence quenching of acetylcholinesterase conjugated Au@BSA clusters

Cited by 32 publications

References 36 publications

Synthesis and bioactivity of several new hetaryl sulfonamides

Synthesis and bioactivity of several new hetaryl sulfonamides

Green Synthesis of Gluten-Stabilized Fluorescent Gold Quantum Clusters: Application As Turn-On Sensing of Human Blood Creatinine

Esterase-Mediated Highly Fluorescent Gold Nanoclusters and Their Use in Ultrasensitive Detection of Mercury: Synthetic and Mechanistic Aspects

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