Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation

Estrada-Cortés, Eliab; Ortiz, W.; Rabaglino, María Belén; Block, J.; Rae, Owen; Jannaman, Elizabeth; Xiao, Yao; Hansen, Peter J.

doi:10.1096/fj.202100991r

Cited by 17 publications

(9 citation statements)

References 59 publications

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“…A significant change in the glycerophospholipid metabolism pathway was also identified in thigh muscle, which was screened in breast muscle, which may perform similar functions in thigh muscle. Michel et al reported that food supplemented with choline improved the birth weight of cattle by lowering DNA methylation [ 66 ]. The concentration of choline decreased significantly in both breast and thigh muscles in line S chickens ( Figure 4 ), which suggested that selection accelerated the consumption of choline and promoted muscle development.…”

Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

et al. 2022

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The selection for improved body weight is an effective approach in animal breeding. Guangxi Partridge chickens have differentiated into two lines under selective breeding, which include line S and line D that have shown statistically significant differences in body weight. However, the meat quality analysis in our study indicated that the quality of breast and thigh muscles in line S chickens changed, which included increased values of L*, b*, and drip loss and decreased a* value, pH, and shear force in skeletal muscles. To illuminate the effect of selection on skeletal muscles, LC-MS/MS metabolomics was performed to explore differentiated metabolites in divergent tissues from the two chicken lines. The results of principal component analysis and orthogonal projection to latent structures discriminant analysis suggested that metabolites of different groups were separated, which suggested that selective breeding certainly affected metabolism of skeletal muscles. KEGG analysis identified that valine, leucine, and isoleucine biosynthesis, glycerophospholipid metabolism, and glutathione metabolism noteworthily changed in breast muscle. Amino sugars and nucleotide sugar metabolism, ascorbate and aldarate metabolism, the pentose phosphate pathway, pentose and glucuronate interconversions, fructose and mannose metabolism, and glycerophospholipid metabolism were remarkedly identified in thigh muscle. These screened pathways suggested oxidative stress in breast and thigh muscles, which corresponded with our previous results. Therefore, this study determined that glycerophospholipid metabolism conservatively functioned in muscle flavor and development but exhibited different anti-oxidative patterns in different skeletal muscles. Overall, the present study identified several differentiated metabolites and pathways for exploring differences in meat quality between different broiler populations.

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Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

et al. 2022

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“…Moreover, studies reported that the maternal diet during gestation affects the DNA methylation pattern in the placenta and offspring of mice (Ge et al, 2014;Mahajan et al, 2019;Zhang et al, 2021a), cattle (Liu et al, 2021), and humans (Daniels et al, 2020;Kupers et al, 2022). Interestingly, a study showed that the exposure of IVP embryos to choline in the culture medium alters the DNA methylation profile in the offspring muscle (Estrada-Cortes et al, 2021). However, these studies evaluated the effects during embryogenesis.…”

Section: Discussionmentioning

confidence: 99%

Differentially methylated regions identified in bovine embryos are not observed in adulthood

Vargas

Nochi²,

Castro

et al. 2023

Anim. Reprod.

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The establishment of epigenetic marks during the reprogramming window is susceptible to environmental influences, and stimuli during this critical stage can cause altered DNA methylation in offspring. In a previous study, we found that low levels of sulphur and cobalt (low S/Co) in the diet offered to oocyte donors altered the DNA methylome of bovine embryos. However, due to the extensive epigenetic reprogramming that occurs during embryogenesis, we hypothesized that the different methylation regions (DMRs) identified in the blastocysts may not maintain in adulthood. Here, we aimed to characterize DMRs previously identified in embryos, in the blood and sperm of adult progenies of two groups of heifers (low S/Co and control). We used six bulls and characterized the DNA methylation levels of KDM2A, KDM5A, KMT2D, and DOT1L genes. Our results showed that all DMRs analysed in both groups and tissues were hypermethylated unlike that noticed in the embryonic methylome profiles. These results suggest that embryo DMRs were reprogrammed during the final stages of de novo methylation during embryogenesis or later in development. Therefore, due to the highly dynamic epigenetic state during early embryonic development, we suggest that is essential to validate the DMRs found in embryos in adult individuals.

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“…Holstein heifers derived from embryos treated with CSF2 from day 5 to day 7 of development had similar birth weights as heifers derived from control embryos but experienced accelerated body growth after 3 months of age ( Kannampuzha-Francis et al, 2015 ). In another experiment ( Estrada-Cortés et al, 2021a ), addition of the methyl donor choline to culture medium of IVP embryos resulted in an increase in birth and weaning weight in Brahman cattle ( Fig. 5 ).…”

Section: Optimizing Postnatal Phenotypementioning

confidence: 94%

“…Transfer of the IVP embryo can also improve fertility in certain situations such as during heat stress and for the repeat-breeder cow and, as discussed by Hansen (2020a) , improvements in the technologies associated with embryo production could result in fertility enhancement more broadly. Furthermore, the preimplantation embryo is amenable to developmental programming; culture media can be modified to produce offspring with phenotypes desirable for production ( Estrada-Cortés et al, 2021a ; 2021b ).…”

Section: Toward the Goal Of An In Vitro -Produced ...mentioning

confidence: 99%

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Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 17 publications

References 59 publications

Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

Differentially methylated regions identified in bovine embryos are not observed in adulthood

Review: Some challenges and unrealized opportunities toward widespread use of the in vitro-produced embryo in cattle production

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