Cholesterol-Targeted Anticancer and Apoptotic Effects of Anionic and Polycationic Amphiphilic Cyclodextrin Nanoparticles

Varan, Gamze; Öncül, Selin; Ercan, Ayşe; Benito, Juan M.; Mellet, Carmen Ortiz; Bilensoy, Erem

doi:10.1016/j.xphs.2016.06.021

Cited by 32 publications

(38 citation statements)

References 53 publications

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“…The presence of chitosan in the aqueous phase may cause a charge interaction between the adsorbed drug on the surface of the nanoparticles and the chitosan, resulting in a more rigid structure. In another previous study, it was reported that the new amphiphilic CD derivative PC βCDC6 is suitable to form stable nanoparticles with small particle size [ 26 ]. The particle size of nanoparticulate drug delivery systems play a direct and important role on cellular uptake, systemic circulation, toxicity and stability of nanoparticles [ 37 – 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…Our goal is to evaluate the potential of the polycationic CD nanoparticles as an anticancer drug delivery system. In fact, these polycationic CDs were evaluated for their intrinsic apoptotic effect in our first paper [ 26 ] in unloaded blank nanoparticle form. This study focuses on the nanocarrier properties and drug delivery system potential of the polycationic CD nanoparticles for PCX, which is an anticancer drug with several serious bioavaibility and toxicity problems.…”

Section: Introductionmentioning

confidence: 99%

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Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

Varan¹,

Benito²,

Mellet³

et al. 2017

Beilstein J. Nanotechnol.

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Background: Paclitaxel is a potent anticancer drug that is effective against a wide spectrum of cancers. To overcome its bioavailability problems arising from very poor aqueous solubility and tendency to recrystallize upon dilution, paclitaxel is commercially formulated with co-solvents such as Cremophor EL® that are known to cause serious side effects during chemotherapy. Amphiphilic cyclodextrins are favored oligosaccharides as drug delivery systems for anticancer drugs, having the ability to spontaneously form nanoparticles without surfactant or co-solvents. In the past few years, polycationic, amphiphilic cyclodextrins were introduced as effective agents for gene delivery in the form of nanoplexes. In this study, the potential of polycationic, amphiphilic cyclodextrin nanoparticles were evaluated in comparison to non-ionic amphiphilic cyclodextrins and core–shell type cyclodextrin nanoparticles for paclitaxel delivery to breast tumors. Pre-formulation studies were used as a basis for selecting the suitable organic solvent and surfactant concentration for the novel polycationic cyclodextrin nanoparticles. The nanoparticles were then extensively characterized with particle size distribution, polydispersity index, zeta potential, drug loading capacity, in vitro release profiles and cytotoxicity studies. Results: Paclitaxel-loaded cyclodextrin nanoparticles were obtained in the diameter range of 80−125 nm (depending on the nature of the cyclodextrin derivative) where the smallest diameter nanoparticles were obtained with polycationic (PC) βCDC6. A strong positive charge also helped to increase the loading capacity of the nanoparticles with paclitaxel up to 60%. Interestingly, cyclodextrin nanoparticles were able to stabilize paclitaxel in aqueous solution for 30 days. All blank cyclodextrin nanoparticles were demonstrated to be non-cytotoxic against L929 mouse fibroblast cell line. In addition, paclitaxel-loaded nanoparticles have a significant anticancer effect against MCF-7 human breast cancer cell line as compared with a paclitaxel solution in DMSO. Conclusion: According to the results of this study, both amphiphilic cyclodextrin derivatives provide suitable nanometer-sized drug delivery systems for safe and efficient intravenous paclitaxel delivery for chemotherapy. In the light of these studies, it can be said that amphiphilic cyclodextrin nanoparticles of different surface charge can be considered as a promising alternative for self-assembled nanometer-sized drug carrier systems for safe and efficient chemotherapy.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

Varan¹,

Benito²,

Mellet³

et al. 2017

Beilstein J. Nanotechnol.

Self Cite

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“…Cancer cells have a high proliferation rate. Thus, the membranes of these cells are synthesized rapidly, which requires more nutrients and leads to higher CHO content of the membrane [22,23].…”

Section: Introductionmentioning

confidence: 99%

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

et al. 2020

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Apoptosis signaling pathways, drug resistance, and metastasis are important targets to develop new cancer treatments. We developed cholesterol-coated Poly(d,l-Lactide-co-Glycolic Acid) (PLGA) nanoparticles for effective encapsulation and delivery of retinoic acid and oxaliplatin to analyze their antitumor activity in colorectal cancer. The cell viability and proliferation of tumoral cells lines (CT-26 and SW-480) decreased when compared to control in vitro after treatment with the nanoparticles. In addition, apoptosis of CT-26 cells increased. Importantly, cytoprotection of nontumor cells was detected. Expression of pro-apoptotic proteins was upregulated, while anti-apoptotic proteins were downregulated either in vitro or in vivo. In addition, drug resistance and metastasis factors were downregulated in vivo. Human colorectal tumors that highly expressed BCL-2 and Ki-67 had a greater tendency towards death within 60 months. Our results show that loading oxaliplatin combined with retinoic acid and cholesterol in a nanoparticle formulation enables determination of optimal antitumor activity and subsequent treatment efficacy.

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“…Today the medications and nutritional supplements factories pay a special attention to natural resources, and tend to use a large amount of local flora into the production. Besides that, efforts have been gradually more to use herbal drugs as substitute medicine [9][10][11][12][13][14][15]. In this aspect, different recent studies have sought after to assess conventional uses and in vitro detection to identify and isolate their active compound for developing drugs from herbal origion [16,17].…”

Section: Discussionmentioning

confidence: 99%

“…For all investigated test (biochemical, enzymetic and immunological assessments), blood was collected by heart puncture, transferred to Eppendorf tube and allowed to clot at room temperature for 15 minutes, and then serum was separated by centrifugation at 3,000 rpm for 10 minutes. That collected serum was used for the assessment of all tested parameters [10,11]. To carry out these assays (enzyme, biochemical and immunological activity) which determined in mouse serum by following the enzymatic colorimetric method of Ref.…”

Section: Assessments Of Biochemical Enzymetic and Immunological Actimentioning

confidence: 99%

Biochemical, Enzymatic, and Immunological Study on Antimutagenic Achillea millefolium Methanolic Extract in vivo

Hussein¹,

Al-Ezzy²,

Abdallah³

2019

JPP

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Traditionally medicinal plants are used for its potential chemotherapeutic action and for safety upon the continued use of these plants. Achillea millefolium (AM) is an ancient herb which is used to treat wounds, headaches, disorder in gastrointestinal and hepatobiliary, pain, and inflammation. This study was aimed to investigate the biochemical parameter of antimutagenic methanolic extract for AM in vivo. Plant extract together with MTX decreased the damage caused by the drug on the concentrations of total cholesterol and triglycerides (160.3±2.26 and 108.7±2.71), significant increase in total protein concentration (8.51±0.17, 9.46±0.19 and 10.8±2.71 g\dL) for 100 and 200 mg\kg was observed. Also plant extracts counteract the damage caused by drug through the reduction for both creatinin and albumin concentrations to 1.57±0.03 mg\dL and 4.56±0.09 g\dL, respectively. On enzymatic level, the results of interactions indicated the ability of plant to modulating harmful effects of the drug (57.1±1.3, 130±3.1 and 111.1±2.1 U\L) for GOT, GPT and ALP, respectively. On the other hand, the plant possesses the ability to enhance the concentrations of all immunoglobulin (IgM, IgA and IgG) in the two doses tested. Therefore, the results of this study indicate the antimutagenic potential of AM extract and encouraging its consumption with MTX as one of the promising therapies.

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Cholesterol-Targeted Anticancer and Apoptotic Effects of Anionic and Polycationic Amphiphilic Cyclodextrin Nanoparticles

Cited by 32 publications

References 53 publications

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

Biochemical, Enzymatic, and Immunological Study on Antimutagenic Achillea millefolium Methanolic Extract in vivo

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