Cholesterol–phospholipid interactions, the liquid-ordered phase and lipid rafts in model and biological membranes

McMullen, Todd; Lewis, Richard L.; McElhaney, Ronald N.

doi:10.1016/j.cocis.2004.01.007

Cited by 326 publications

(295 citation statements)

References 38 publications

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“…Sphingolipids differ from glycerophospholipids in that the former have both of their acyl chains saturated. Glycerophospholipids have one unsaturated chain (McMullen et al, 2004;Munro, 2003). The relative concentrations of the three lipid groups typically are 10-20%, 30-40%, and 40-60% (mol.%) for sphingolipids, cholesterol and glycerophospholipids, respectively (McMullen et al, 2004).…”

Section: Model Membranesmentioning

confidence: 99%

“…Glycerophospholipids have one unsaturated chain (McMullen et al, 2004;Munro, 2003). The relative concentrations of the three lipid groups typically are 10-20%, 30-40%, and 40-60% (mol.%) for sphingolipids, cholesterol and glycerophospholipids, respectively (McMullen et al, 2004). Fluidity of the bilayer depends on membrane composition (Alberts et al, 2002).…”

Section: Model Membranesmentioning

confidence: 99%

“…Most, not all of sphingolipids are present in the exoplasmic leaflet while phosphatidylserine, phosphatidylinositol, and phosphatidylethalonamine are restricted to the cytoplasmic leaflet. The distribution of cholesterol is assumed higher, although perhaps only slightly, on the outer than the inner leaflet because of its stronger interaction with sphingolipids than phospholipids (McMullen et al, 2004;Munro, 2003 (McMullen et al, 2004).…”

Section: Model Membranesmentioning

confidence: 99%

“…Figure 3A encompasses our current understanding of the raft model. It shows that sphingolipids and cholesterol are distributed unevenly on the outer leaflet of the membrane and cluster together to form L o domains that float in a bed of a L d bilayer (McMullen et al, 2004;Munro, 2003;Simons and Ikonen, 1997). The sphingolipid molecules associate with each other via their head groups and within the bilayer through the saturated hydrocarbon chains.…”

Section: Lipid Raftmentioning

confidence: 99%

“…The reader is referred to recent reviews by Hanzal-Bayer and Hancock (2007) for an in-depth discussion of the lipid raft hypotheses and the role of rafts in membrane traffic, and McMullen et al (2004) for a thorough discussion on lipid raft composition. Herein, we merely introduce the general structure of lipid rafts and how this is important in understanding the biochemical mechanism(s) behind Ab-induced neurotoxicity in AD.…”

Section: Lipid Raftmentioning

confidence: 99%

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Using model membranes for the study of amyloid beta:lipid interactions and neurotoxicity

Vestergaard

Hamada

Takagi

2008

Biotech & Bioengineering

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One of the major tasks in understanding the etiopathogenesis of amyloid beta-induced neurotoxicity of Alzheimer's disease (AD), is in fully capturing the large number of the biochemical processes that influence each other during the course of the disease, in vivo. Model membranes possess, as their main strength, the ability to enable the researcher to manipulate a 'biological' microvesicle under a controlled environment. This review narrowly focuses on discussing the exploitation of model membranes for improved understanding of some of the mechanisms governing AD's amyloid beta-induced neurotoxicity. Amyloid beta (Ab) is cleaved from a membranelocated amyloid precursor protein by membrane-located enzymes. The relative spatial localization of the involved biomolecules within the membrane bilayer is crucial in influencing Ab production, its aggregation on the membrane surface or insertion into the membrane, and fibril formation: all important processes in causing neurotoxicity. The lipid composition of the bilayer is similarly important. The review also attempts to highlight current and future challenges in using model membranes for studying biochemical processes.

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Section: Model Membranesmentioning

confidence: 99%

Section: Model Membranesmentioning

confidence: 99%

Section: Model Membranesmentioning

confidence: 99%

Section: Lipid Raftmentioning

confidence: 99%

Section: Lipid Raftmentioning

confidence: 99%

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Using model membranes for the study of amyloid beta:lipid interactions and neurotoxicity

Vestergaard

Hamada

Takagi

2008

Biotech & Bioengineering

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Glycosyl chains and 25‐hydroxycholesterol contribute to the intracellular transport of amyloid beta (Aβ‐42) in Jurkat T cells

et al. 2017

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Amyloid beta (Aβ) is a peptide responsible for the development of Alzheimer's disease ( AD ). Misfolding and accumulation of endogenous Aβ can lead to neural cell apoptosis through endoplasmic reticulum ( ER ) stress. Added exogenous Aβ can also result in ER stress, leading to neurotoxicity and apoptosis, which is identical to that caused by the endogenous peptide. We have speculated that the endocytic transport of Aβ causes ER stress and have previously shown that the oxysterol, in particular, 7‐ketocholesterol (7‐keto) induces more surface interaction between Aβ‐42 and Jurkat cells than cholesterol. However, the interaction was not enough to induce intracellular transfer of the peptide. In this study, we investigated the effect of another oxysterol, 25‐hydroxycholesterol (25‐OH) on the membrane raft‐dependent transport of Aβ‐42 in Jurkat cells. Interestingly, intracellular transfer of Aβ‐42 was observed in the presence of 25‐ OH only after the inclusion of cholera toxin B subunit (CT‐B), a marker used to detect the raft domain. We speculated that 25‐ OH can induce intracellular movement of Aβ peptides. Furthermore, CT ‐B together with GM 1 provided negative curvature, which resulted in the intracellular transport of Aβ‐42. Notably, we used a protofibrillar species of Aβ‐42 in this study. We have shown that the transport was microtubule‐dependent since it could not be observed in depolymerized microtubules. These results demonstrate that oxysterols and glycosyl chains are important factors affecting intracellular transport. These compounds are also associated with aging and advanced glycation are risk factors for AD . Thus, this study should further understanding of the pathology of AD .

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Supported Lipid Bilayers: Intelligent Surfaces for Ion Channel Recordings

Janshoff

Steinem

2012

Intelligent Surfaces in Biotechnology

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Cholesterol–phospholipid interactions, the liquid-ordered phase and lipid rafts in model and biological membranes

Cited by 326 publications

References 38 publications

Using model membranes for the study of amyloid beta:lipid interactions and neurotoxicity

Using model membranes for the study of amyloid beta:lipid interactions and neurotoxicity

Glycosyl chains and 25‐hydroxycholesterol contribute to the intracellular transport of amyloid beta (Aβ‐42) in Jurkat T cells

Supported Lipid Bilayers: Intelligent Surfaces for Ion Channel Recordings

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