Cholesterol Metabolism in Two Strains of Rats with High or Low Response of Serum Cholesterol to a Cholesterol-Rich Diet

Abstract

Transfer from a low cholesterol commercial diet to a high cholesterol diet, containing 2% (wt/wt) cholesterol and 0.5% cholate, caused an increase in serum cholesterol from about 2.5 mmol/L in two inbred rat strains to 5 mmol/L in the hyporesponsive strain and to 20 mmol/L in the hyperresponsive strain. In both strains the excess of cholesterol in the serum was exclusively located in the very low density lipoproteins. Cholesterol feeding caused a sevenfold increase in the amount of cholesterol in the liver, the increase tending to be greater in the hyporesponders. On the commercial diet, the decay of specific radioactivity of serum cholesterol after the intravenous administration of labeled cholesterol was faster in the hyporesponsive rats. The rate of fecal excretion of radioactive bile acids on this diet was higher in the hyporesponders when compared with the hyperresponders, whereas there was no strain difference with regard to the output of fecal neutral steroids. Sterol balance data showed that whole-body cholesterol synthesis on the low cholesterol diet was about twofold higher in the hypo- than in the hyperresponders. When fed the high cholesterol diet the half-life in the serum of injected radioactive cholesterol was about six times shorter in the hyporesponders. In absolute amounts, the hypo- and hyperresponders excreted similar amounts of endogenous (radioactive) bile acids and fecal steroids with the feces on this diet.

Effects of Restructured Pork Containing <b><i>Himanthalia elongata</i></b> on Adipose Tissue Lipogenic and Lipolytic Enzyme Expression of Normo- and Hypercholesterolemic Rats

“…Nonetheless, the filling effect of alga fibers was lost when cholesterol was added to the SS diet. In agreement with Beynen et al [23], supplementary dietary cholesterol decreased the growth rate. Lower body weight gain in rabbits but not in rats fed cholesterol-enriched diets has been found [24].…”

The effect of a combined dietary treatment with cholesterol and cholic acid on the lipid metabolism of geese at low or high choline concentrations

“…The most profound effect in this respect was a marked accumulation of cholesterol and triglycérides in the liver which also has been observed in other species such as rats (Morin et al, 1962;Rönnemaa, 1976;Hulbron et al, 1982;Martins and Dhopeswharkar, 1982;Beynen et al, 1984), guinea pigs (Beck and Drevon, 1980), rabbits (Lee and Ho, 1972;Gupta et al, 1976) and mice (Lee, 1981;Reihner and Stahlberg, 1996). The extremely high concentrations of cholesterol and triglycérides in VLDL might be the consequence of a very high rate of lipid secretion from liver into blood.…”

“…Several studies in rats (Rönnemaa, 1976;Hulbron et al, 1982;Martins and Dhopeshwarkar, 1982;Beynen et al, 1984;Huang et al, 1984;Garg et al, 1985;Stangl et al, 1993Stangl et al, , 1994a, hamsters (Ohtani et al, 1990) and rabbits (Gupta et al, 1976) demonstrated that diets rich in cholesterol markedly raise plasma cholesterol 286 K. EDER concentrations. Therefore, dietary treatment with cholesterol is a widely used model for the study of the development of atherosclerosis {e.g., Stangl et al, 1993;1994a;Loria and Padgett, 1997;Munilla and Herrera, 1997;Munday et al, 1998).…”

Chromosomal localization of genes involved in biosynthesis, metabolism or transport of cholesterol in the rat

“…Impaired cholesterol metabolism is one of the predisposing factors in the process of this disease (Beynen et al, 1984;Doucet et al, 1987;Bottger et al, 1996a). In a previous study, involving the F 2 progeny of a cross between two rat inbred strains differing in their response to dietary cholesterol, we have found several quantitative trait loci (QTLs) that are associated with cholesterol and other lipid levels in blood and liver (Bonné et al, submitted).…”