Cholesterol metabolism in monocyte-derived macrophages from macrophage colony-stimulating factor administered rabbits

Ishii, Itsuko; Kimuro, Takashi; Saitō, Yasushi; Hirose, Sachiko

doi:10.1016/0005-2760(94)00163-s

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Assays Of Nceh Activity In Macrophages1

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Cited by 7 publications

(1 citation statement)

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“…nCEH activity was assayed as previously described 18) . Briefly, cell monolayers were washed with PBS before being scraped into 200 L assay buffer (50 mM Tris-HCl, pH 7.4, containing 1 mM EDTA and 0.3 M sucrose).…”

Section: Assays Of Nceh Activity In Macrophagesmentioning

confidence: 99%

Insulin Suppresses HDL-Mediated Cholesterol Efflux from Macrophages Through Inhibition of Neutral Cholesteryl Ester Hydrolase and ATP-Binding Cassette Transporter G1 Expressions

Yamashita

Tamasawa

Matsuki

et al. 2010

JAT

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Section: Assays Of Nceh Activity In Macrophagesmentioning

confidence: 99%

Insulin Suppresses HDL-Mediated Cholesterol Efflux from Macrophages Through Inhibition of Neutral Cholesteryl Ester Hydrolase and ATP-Binding Cassette Transporter G1 Expressions

Yamashita

Tamasawa

Matsuki

et al. 2010

JAT

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Acrolein-conjugated low-density lipoprotein induces macrophage foam cell formation

et al. 2013

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Marked induction of sterol 27-hydroxylase activity and mRNA levels during differentiation of human cultured monocytes into macrophages

Hansson

Ellis

Hunt

et al. 2003

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Sterol 27-hydroxylase has been suggested to be involved in an alternative pathway for the elimination of cholesterol from macrophages and early atherosclerotic lesions. We have previously shown that human lung macrophages as well as monocyte-derived macrophages have a relatively high activity of sterol 27-hydroxylase (CYP27). This enzyme converts intracellular cholesterol into 27-hydroxycholesterol and cholestenoic acid that flux from cultured cells into the medium. It is shown here that human monocytes have very low CYP27 activity and CYP27 mRNA levels. During differentiation into macrophages, both CYP27 activity and CYP27 mRNA levels increase markedly after 4 days of culture in serum-free medium. Addition of macrophage-colony stimulating factor had no significant effect on the induction and addition of fetal calf serum had an inhibitory effect. Cholesterol synthesis was found to be a critical factor for the production of 27-oxygenated products by the macrophages cultured in serum-free medium. The increased capacity of the differentiated cells to eliminate intracellular cholesterol is of interest and supports the contention that CYP27 is an antiatherogenic factor.

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Cholesterol metabolism in monocyte-derived macrophages from macrophage colony-stimulating factor administered rabbits

Cited by 7 publications

References 14 publications

Insulin Suppresses HDL-Mediated Cholesterol Efflux from Macrophages Through Inhibition of Neutral Cholesteryl Ester Hydrolase and ATP-Binding Cassette Transporter G1 Expressions

Insulin Suppresses HDL-Mediated Cholesterol Efflux from Macrophages Through Inhibition of Neutral Cholesteryl Ester Hydrolase and ATP-Binding Cassette Transporter G1 Expressions

Acrolein-conjugated low-density lipoprotein induces macrophage foam cell formation

Marked induction of sterol 27-hydroxylase activity and mRNA levels during differentiation of human cultured monocytes into macrophages

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