Cholesterol lowering and inhibition of sterol absorption by Lactobacillus reuteri NCIMB 30242: a randomized controlled trial

Abstract

BACKGROUND/OBJECTIVES:The percentage of hypercholesterolemic individuals not reaching their LDL-cholesterol (LDL-C) goal remains high and additional therapeutic strategies should be evaluated. The objective of this study was to evaluate the cholesterollowering efficacy and mechanism of action of bile salt hydrolase-active Lactobacillus reuteri NCIMB 30242 capsules in hypercholesterolemic adults. SUBJECTS/METHODS: A total of 127 subjects completed a randomized, double-blind, placebo-controlled, parallel-arm, multicenter study. Subjects were randomized to consume L. reuteri NCIMB 30242 capsules or placebo capsules over a 9-week intervention period. The primary outcome was LDL-C relative to placebo at the study end point. RESULTS: L. reuteri NCIMB 30242 capsules reduced LDL-C by 11.64% (Po0.001), total cholesterol by 9.14%, (Po0.001), non-HDLcholesterol (non-HDL-C) by 11.30% (Po0.001) and apoB-100 by 8.41% (P ¼ 0.002) relative to placebo. The ratios of LDL-C/HDLcholesterol (HDL-C) and apoB-100/apoA-1 were reduced by 13.39% (P ¼ 0.006) and 9.00% (P ¼ 0.026), respectively, relative to placebo. Triglycerides and HDL-C were unchanged. High-sensitivity C-reactive protein and fibrinogen were reduced by 1.05 mg/l (P ¼ 0.005) and 14.25% (P ¼ 0.004) relative to placebo, respectively. Mean plasma deconjugated bile acids were increased by 1.00 mmol/l (P ¼ 0.025) relative to placebo, whereas plasma campesterol, sitosterol and stigmasterol were decreased by 41.5%, 34.2% and 40.7%, respectively. CONCLUSIONS: The present results suggest that the deconjugation of intraluminal bile acids results in reduced absorption of non-cholesterol sterols and indicate that L. reuteri NCIMB 30242 capsules may be useful as an adjunctive therapy for treating hypercholesterolemia.

Effectiveness of probiotics in type 2 diabetes: a meta-analysis

“…31, 32 Hulston et al 33 reported that probiotic consumption has a positive effect on blood glucose concentrations and insulin sensitivity in healthy subjects on obesogenic diet. Furthermore, studies have demonstrated that probiotics may have potential benefits for the prevention of other diabetes-related changes, 34-37 which has been confirmed, at least partially, by our meta-analysis.…”

Evaluation of probiotic potential of lactic acid bacteria isolated from traditional Malaysian fermentedBambangan(Mangifera pajang)

“…A large number of LAB, isolated from various fermented foods in different parts of the world, had been studied for their probiotic potential and ability to produce important substances for foods and pharmaceutical preparation (BautistaGallego et al, 2013;Monteagudo-Mera et al, 2012;Tham, Peh, Bhat, & Liong, 2012). LAB have become the focus of probiotic research internationally and their health benefits in the prevention of diarrhoea (Hempel et al, 2012), reduction of the cholesterol level (Jones, Martoni, & Prakash, 2012), relief of both irritable bowel syndrome (Ducrotté, Sawant, & Jayanthi, 2012) and milk allergy in infants (Kekkonen et al, 2008) are well documented. However, these properties cannot be extrapolated or generalised for all probiotic strains as such effects are strain-specific and a considerable number of works are still required to affirm the benefits of each probiotic candidate (Aureli et al, 2011).…”

Growth of Lactobacillus reuteri NCIMB 30242 during yogurt fermentation and bile salt hydrolysis activity in the product

“…Fermented milks are recognized as being very good matrices for functional ingredients (Shiby and Mishra 2012). Lactobacillus reuteri NCIMB 30242 has been shown to be clinically efficacious on reducing blood cholesterol (Jones et al 2012a(Jones et al , 2012d.…”