Cholesterol-loaded cyclodextrin enhances osmotic tolerance and inhibits the acrosome reaction in rabbit spermatozoa

Aksoy, Melih; Akman, Orhan; Lehimcioğlu, Necdet Cankat; Erdem, Hüseyin

doi:10.1016/j.anireprosci.2010.02.014

Cited by 35 publications

(20 citation statements)

References 35 publications

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“…The solution was kept in a water bath for 10 min at 39°C and then vortexed and sonicated for 30 min. The working solution of CLC was prepared by adding BSA (3 mg/mL) as reported earlier by Aksoy et al [3].…”

Section: Clc Preparationmentioning

confidence: 99%

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Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

et al. 2015

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Section: Clc Preparationmentioning

confidence: 99%

“…The exact mechanism of action of CLC, involved in sperm protection during freezing and thawing procedure is not well understood. However, its role in enhancing osmotic tolerance and reducing premature capacitation/acrosome reaction have been elucidated in simulation studies in different species [2,3,12,24,45,36].…”

Section: Introductionmentioning

confidence: 99%

Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

et al. 2015

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“…This might be due to the fact that increased cholesterol in the sperm cell membrane enhanced the osmotic tolerance and inhibited the acrosome reaction (52). On the other hand, fertility after use of CLCtreated frozen-thawed semen is questionable.…”

Section: β-Cyclodextrinmentioning

confidence: 99%

Cholesterol efflux from sperm: approaches and applications

Naseer¹,

Ahmad²,

Aksoy³

2014

Turk J Vet Anim Sci

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Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides insight to researchers designing new procedures for sperm preparation to be used in assisted reproductive technology.

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“…In addition, cholesterol has a complex effect on plasma membrane property. For instance, it decreases membrane permeability and phase changes, provides a proper physical and/or chemical microenvironment for membrane proteins, regulates morphological characteristics and acts as a membrane antioxidant [16,17] . Cyclodextrins are cyclic oligosaccharides, which are the main degradation products of starch.…”

Section: Introductionmentioning

confidence: 99%

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

̇İnanç¹,

Olğaç²,

Tekin³

et al. 2018

Kafkas Univ Vet Fak Derg

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How to Cite This Articleİnanç ME, Olgac KT, Tekin K, Çil B, Alemdar H, Özen D, Uysal O: Effect of cholesterol and 7-dehydrocholesterol on bull semen freezing with different rates of glycerol. Kafkas Univ Vet Fak Derg, 24 (6): 815-820, 2018. DOI: 10.9775/kvfd.2018 AbstractThe aim of this study was to evaluate the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol loaded cyclodextrin (CLC) with the addition of different glycerol (G) rates on bull semen cryopreservation. Semen samples of three bulls were pooled and divided into seven groups; control group (C) and the other six were extended with standard Tris extender including different G rates (3, 6 and 9%) with CLC or 7-DHCLC to reach 1.5 mg/120x10 6 . Extended samples were frozen in 0.25 mL straws. After thawing, motion characteristics and motility, viability, acrosome integrity, mitochondrial activation, functional membrane integrity and abnormal spermatozoa rates were assessed. The highest membrane integrity, viability and total motility were detected in 7-DHCLC with 9% of G group (P<0.001). The lowest progressive motility was detected in 3% G groups (P<0.001). 7-DHCLC with 9% G had higher mitochondrial activity compared with 3% G groups. In addition, no statistical difference was observed between groups in terms of acrosome integrity and abnormal spermatozoa rates (P>0.05). According to these results, 7-DHCLC with 9% G addition maintained some spermatological parameters better than other groups after thawing in bull semen thus, it was concluded that 7-DHCLC could be used instead of CLC in bull semen cryopreservation. Keywords: Cholesterol 7-dehydrocholesterol, Cyclodextrin, Glycerol, Bull semen, Freeze-thawed Kolestrol ve 7-Dehidrokolestrolün Farklı Gliserol Oranları İle Boğa Sperması Dondurulmasına Etkisi ÖzBu çalışmanın amacı, 7-dehidrokolesterol yüklü siklodekstrin (7-DHCLC) ve kolesterol yüklü siklodekstrin (CLC) 'in farklı gliserol (G) oranları ilavesi ile boğa sperması dondurulması üzerindeki etkilerini değerlendirmektir. Üç adet boğaya ait sperma örnekleri toplandı, birleştirildi ve yedi gruba ayrıldı; biri kontrol (C) ile, diğer altı grup ise CLC veya 7-DHCLC (1.5 mg/120x10 6 ) ve farklı G oranlarını (%3, 6 ve 9) içeren standart Tris sulandırıcısı ile sulandırıldı. Sulandırılan spermalar 0.25 mL'lik payetlerde donduruldu. Çözdürme sonrası gruplar hareket karakteristikleri ve motilite, viabilite, akrozom bütünlüğü, mitokondriyal aktivasyon, fonksiyonel membran bütünlüğü ve anormal spermatozoa oranları yönünden değerlendirildi. En yüksek membran bütünlüğü, viabilite ve motilite %9 G ilavesi ile 7-DHCLC grubunda tespit edildi (P<0.001). En düşük progresif motilite %3 G gruplarında saptandı (P<0.001). %9 G içeren 7-DHCLC grubu, %3 G gruplarına kıyasla daha yüksek mitokondriyal aktivite belirlendi. Ayrıca, gruplar arasında akrozom bütünlüğü ve anormal spermatozoa oranları açısından istatistiksel bir fark gözlenmedi (P>0.05). Bu sonuçlara göre, %9 G ilave edilen 7-DHCLC grubunun, diğer gruplara kıyasla eritme sonrası bazı spermat...

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Cholesterol-loaded cyclodextrin enhances osmotic tolerance and inhibits the acrosome reaction in rabbit spermatozoa

Cited by 35 publications

References 35 publications

Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

Cholesterol efflux from sperm: approaches and applications

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

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