Cholesterol concentrations in lipoprotein fractions separated by anion-exchange–high-performance liquid chromatography in healthy dogs and dogs with hypercholesterolemia

Oda, Hitomi; Mori, Akira; Hirowatari, Yuji; Takoura, Toshie; Manita, Daisuke; Takahashi, Tomoya; Shono, Saori; Onozawa, Eri; Mizutani, H.; Miki, Yohei; Itabashi, Yukiko; Sako, Toshinori

doi:10.1016/j.rvsc.2017.04.004

Cited by 7 publications

(14 citation statements)

References 25 publications

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“…Manual and automated lipoprotein electrophoresis, density‐gradient ultracentrifugation, various high‐performance liquid chromatography methods, and chemistry analyzers have been used to quantify lipoproteins in dogs. There is currently no established consensus on the gold standard methodology for lipoprotein measurements in dogs . Therefore, it is possible that lipoprotein concentrations measured by a different methodology, such as manual lipoprotein electrophoresis, could yield somewhat different results than those reported in this study.…”

Section: Discussionmentioning

confidence: 72%

“…There is currently no established consensus on the gold standard methodology for lipoprotein measurements in dogs. 5,[22][23][24][25] Therefore, it is possible that lipoprotein concentrations measured by a different methodology, such as manual lipoprotein electrophoresis, could yield somewhat different results than those reported in this study. However, the goal of this study was to compare lipoprotein lipid concentrations in dogs with and without DM.…”

Section: Discussionmentioning

confidence: 95%

“…Several methods for lipoprotein quantification have been reported in dogs, with issues such as ease of use, equipment availability, time constraints, and accuracy being considered. 5,13,[22][23][24][25] Manual and automated lipoprotein electrophoresis, density-gradient ultracentrifugation, various high-performance liquid chromatography methods, and chemistry analyzers have been used to quantify lipoproteins in dogs. There is currently no established consensus on the gold standard methodology for lipoprotein measurements in dogs.…”

Section: Discussionmentioning

confidence: 99%

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Spectrophotometry and Ultracentrifugation for Measurement of Plasma Lipids in Dogs with Diabetes Mellitus

Seage

Drobatz

Hess

2017

Veterinary Internal Medicne

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BackgroundThere are conflicting reports of plasma lipoprotein lipid content in dogs with diabetes mellitus (DM).ObjectivesTo determine lipoprotein lipid content of plasma of dogs with DM by spectrophotometry and ultracentrifugation; to compare lipoprotein lipid content in diabetic and healthy dogs; and to quantify apolipoprotein B‐100 (ApoB) in dogs with DM.Animals22 dogs with DM and 9 healthy dogs.MethodsCross‐sectional study. Triglyceride (TG), total cholesterol (TC), and high‐density lipoprotein cholesterol (HDL‐C) concentrations were measured by spectrophotometry. Very low‐density lipoprotein cholesterol (VLDL‐C) and low‐density lipoprotein cholesterol (LDL‐C) concentrations were calculated after ultracentrifugation. Non‐HDL‐C cholesterol was calculated by subtracting HDL‐C from TC. ApoB was quantified by ELISA. The Mann‐Whitney test was used for comparison of median lipoprotein concentrations, and Spearman's correlation was used to assess associations between ApoB and lipoprotein fractions.ResultsAll values are reported in mg/dL. Median TG (122), TC (343.5), HDL‐C, (200), VLDL‐C, (27) LDL‐C (68), non‐HDL‐C (114), and ApoB (320) were significantly higher in dogs with DM, compared to healthy dogs (57, 197, 168, 12, 16, 31, and 258, respectively, P‐values 0.0079, <0.001, 0.029, 0.011, <0.001, <0.001, 0.025, respectively). A significant association was found between ApoB and LDL‐C (Spearman's rho = 0.41, P = 0.022) and between ApoB and non‐HDL‐C (Spearman's rho = 0.40, P = 0.027).Conclusions and Clinical ImportanceDyslipidemia of dogs with DM is characterized by pronounced increases in LDL‐C and non‐HDL‐C concentrations, although all lipoprotein fractions are significantly increased. Knowledge of specific lipoprotein fraction alterations in dogs with DM can enhance treatment options for diabetic dyslipidemia in dogs.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Spectrophotometry and Ultracentrifugation for Measurement of Plasma Lipids in Dogs with Diabetes Mellitus

Seage

Drobatz

Hess

2017

Veterinary Internal Medicne

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“…If one of these components is impaired, dyslipidemia may occur and lead to various lipid deposits in tissues, atherosclerosis and/or cardiovascular diseases in humans [1,5,6]. Except for Intermediate-Density Lipoproteins (IDL) [7], all kinds of lipoproteins with overall same molecular characteristics are found in dogs, cats and horses [8] but surprisingly, primary atherosclerotic dyslipidemia are rarely observed in these domestic animals. The present mini review aims to compare the apolipoproteins components with human molecules, to emphasize some marked differences in cholesterol transport and to correlate the major dyslipidemias occurring in the three animal species to eventual anomalies in the lipoprotein metabolism.…”

Section: Introductionmentioning

confidence: 99%

"Lipoproteins and Cholesterol Transport in Dogs, Cats and Horses: Particular Feature Compared to Humans (Mini Review)"

Bret¹

2022

BJSTR

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Lipoproteins are large particles of lipids stabilized by apolipoproteins and they transport lipids in the plasma within the body by specific oriented pathways according to the kind of the lipoproteins. Apolipoproteins have structural and functional roles: they serve as recognition molecules for membrane receptors and are essential partners for enzymes (LPL, LCAT and CETP) that are involved in the metabolism and exchange of lipids. The composition of lipoproteins is slightly different between dogs, cats, horses and humans: VLDLs containing the ApoB48 molecule are identified in dogs and horses. The HDL lipoproteins are the major lipoproteins in animals, but the spherical triglyceride-rich HDL particles are virtually absent because the CETP activity is low. By limiting triglyceride exchanges between lipoproteins, these compositional differences impact the prevalence of atherosclerosis in animals in which the vascular lesions occur secondary to other endocrine and metabolic disorders.

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“…Foram avaliadas cadelas obesas com ECC 8-9 e % de MG igual a 32,48 ± 6,43, mas as concentrações de colesterol total, triglicérides e LDL não ultrapassaram os valores de referência (JERICÓ et al, 2009;ODA et al, 2017). Entretanto, os benefícios do emagrecimento foram novamente constatados, a partir da melhora do perfil lipídico, com diminuição das concentrações de colesterol total, triglicérides, LDL e VLDL.…”

Section: Correlaçõesunclassified

Avaliação cardiovascular e determinação de fatores ateroscleróticos em cães obesos

Roque¹

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Primeiramente agradeço a minha mãe Rita, por sempre ter me apoiado nas escolhas que fiz e dividir comigo o sonho de ser Médica Veterinária. Ao meu namorado Murilo, por me escutar todas às vezes mesmo a distância e por não me deixar desistir. À Profª. Drª. Deise Carla Almeida Leite Dellova, pelas oportunidades oferecidas, pelos ensinamentos ao longo destes anos de convívio. E por ser essa pessoa que me faz querer ser melhor todos os dias. Meu exemplo de profissional. Ao Prof. Dr. Márcio Brunetto e seus orientados por me mostrarem a importância da nutrição. Ao Prof. Dr. Júlio Cesar, por me ajudar a entender um pouco de estatística. À técnica de laboratório M. V. Danielle Passarelli, por toda ajuda com as análises e pela paciência. A cardiologista M. V. Érika por me auxiliar nos exames ecocadiodiográficos. Às estagiárias do serviço de eletrocardiograma Amanda, Maíra, Adriana, Marcela e Fernanda por terem me ajudado em todas as coletas. A todos os funcionários do hospital veterinário UDCH, em especial a M.V Paula e técnica Bárbara, pela ajuda diária no atendimento. Aos jovens veterinários (Karina, Bruna, Henrique, William e Samira), pelo apoio nas avaliações nutricionais. Aos grupos de estudos e integrantes GEPA e ENDOCRINOPET por me manterem sempre estudando. Aos laboratórios Hermes Pardini e Cabianca, principalmente a Ana Carolina e a Drª Eugenia, pela atenção e auxílio das análises. Ao laboratório de Espectrometria de Massa da Faculdade de Medicina de Ribeirão Preto e a nutricionista Karina, por me ajudar com as análises. À Faculdade de Zootecnia e Engenharia de Alimentos, pela oportunidade de realização do curso de mestrado. À Fundação de Amparo à Pesquisa do Estado de São Paulo pela concessão da bolsa de mestrado e pelo apoio financeiro para a realização desta pesquisa, possibilitando assim a permanência na cidade de Pirassununga para atividade integral e exclusiva ao projeto, e também a compra de todos os matérias e serviços necessários com a reserva técnica (Número processo: 2014/27242-7). À Nestlé Purina, em especial a Jacira, por viabilizar a padronização da dieta usada no projeto. À minha prima Gabriela, por dividir comigo um lar. Às minhas amigas Gabriela e Laís, por todas as risadas, conversas e incentivos. À minha querida amiga Shayne Pedrozo Bisetto, pela amizade, companhia e compreensão em todos os momentos. Aos tutores que permitiram a avaliação de seus animais e também ao comprometimento à pesquisa. E finalmente às minhas especiais cadelas (magrinhas e gordinhas) que participaram do estudo, sem elas não seria possível todo esse trabalho. "O impossível não é uma fato: e uma opinião". (Mario Sergio Cortella) RESUMO ROQUE, B. K. Avaliação cardiovascular e determinação de fatores ateroscleróticos em cães obesos. 2017. 89 f. Dissertação (Mestrado)-Faculdade

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Cholesterol concentrations in lipoprotein fractions separated by anion-exchange–high-performance liquid chromatography in healthy dogs and dogs with hypercholesterolemia

Cited by 7 publications

References 25 publications

Spectrophotometry and Ultracentrifugation for Measurement of Plasma Lipids in Dogs with Diabetes Mellitus

Spectrophotometry and Ultracentrifugation for Measurement of Plasma Lipids in Dogs with Diabetes Mellitus

"Lipoproteins and Cholesterol Transport in Dogs, Cats and Horses: Particular Feature Compared to Humans (Mini Review)"

Avaliação cardiovascular e determinação de fatores ateroscleróticos em cães obesos

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