Cholesterol, But Not Cigarette Smoke, Decreases Rabbit Carotid Artery Relaxation

Johnson, Dawn; Freischlag, Julie A.; Coe, Douglas A.; Mudaliar, Junaid H.; Traul, David K.; Kelly, Holly; Hanson, Lara; Cambria, Robert A.; Seabrook, Gary R.; Towne, Jonathan B.

doi:10.1007/s100169900287

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…[2][3][4][5][6] However, the precise mechanism of cigarette smoke-mediated endothelial dysfunction and the alteration of NO biology is unclear, and existing data are often contradictory. [2][3][4][5][6][7][8][9][10][11][12][13] The majority of in vitro studies related to smoking used crude cigarette smoke extract (CSE) produced by passing cigarette smoke through a buffer solution. 6,9,14 This model is relevant but probably not physiological.…”

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confidence: 99%

Dysfunctional Endothelial Nitric Oxide Biosynthesis in Healthy Smokers With Impaired Endothelium-Dependent Vasodilatation

et al. 2001

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Background-The mechanisms involved in the dysfunction of both endothelium-dependent vasodilatation (EDV) and NO biosynthesis related to smoking are unclear. In this study, EDV was assessed in healthy smokers and nonsmokers in vivo and, using serum from the same individuals, was related to the NO biosynthetic pathway in vitro. Methods and Results-Flow-mediated EDV of the brachial artery was measured in 23 male patients (8 nonsmokers and 15 smokers). Serum was collected, added to confluent (Ϸ85%) monolayers of human umbilical vein endothelial cells (HUVECs), and incubated for 12 hours. Basal and substance P-stimulated NO production was measured. The HUVECs used for measuring basal NO production were lysed, and both endothelial NO synthase (eNOS) protein expression and eNOS activity were determined. EDV was lower in smokers compared with nonsmokers (PϽ0.001). HUVECs treated with serum from smokers compared with nonsmokers showed significantly lower basal (PϽ0.0001) and stimulated (PϽ0.02) NO production, higher eNOS expression (PϽ0.0001), but lower eNOS activity (PϽ0.004). There was a significant positive correlation between in vivo EDV and in vitro substance P-stimulated NO production (rhoϭ0.57, PϽ0.01) and between basal NO production and eNOS activity (rϭ0.54, PϽ0.008) and a negative correlation between basal NO production and eNOS protein expression (rϭϪ0.60, PϽ0.003). Conclusions-This is the first study to combine an in vivo model with a near-physiological in vitro model to demonstrate an association between decreased NO production and reduced EDV. Cigarette smoking was associated with reduced EDV, NO generation, and eNOS activity in the presence of increased eNOS protein expression.

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confidence: 99%

Dysfunctional Endothelial Nitric Oxide Biosynthesis in Healthy Smokers With Impaired Endothelium-Dependent Vasodilatation

et al. 2001

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“…The relatively short duration, and thus the relatively low dose, of cigarette smoke exposure (relative to a lifetime) may have accounted for the paucity of ductal hyperplasia. In animal models, 600 cigarettes/day for 5 days/ week has correlated with serum cotinine, a major metabolite of nicotine, and carboxyhemoglobin levels observed in chronic smokers [17]. In this study, however, this concentration during just a 3-month period in the hamster most likely approximated only a fraction of a chronic smoker's lifetime exposure.…”

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confidence: 41%

Histopathologic changes induced by cellophane wrapping of the pancreas are unaffected by cigarette smoke exposure in hamsters

et al. 2002

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“…Além disso, a normalidade do fluxo sangüíneo é descrita como fundamental para a homeostase do endotélio, com implicação nas propriedades elásticas arteriais (2,28,29) . Os efeitos da exposição ao CO conta com vasta literatura, por vezes conflitante, sobre o sistema cardiovascular (17,18,19) .…”

Section: Análise Estatísticaunclassified

"Efeitos do tabagismo sobre o sistema cardiovascular: hemodinâmica e propriedades elásticas arteriais"

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Cholesterol, But Not Cigarette Smoke, Decreases Rabbit Carotid Artery Relaxation

Cited by 7 publications

References 24 publications

Dysfunctional Endothelial Nitric Oxide Biosynthesis in Healthy Smokers With Impaired Endothelium-Dependent Vasodilatation

Dysfunctional Endothelial Nitric Oxide Biosynthesis in Healthy Smokers With Impaired Endothelium-Dependent Vasodilatation

Histopathologic changes induced by cellophane wrapping of the pancreas are unaffected by cigarette smoke exposure in hamsters

"Efeitos do tabagismo sobre o sistema cardiovascular: hemodinâmica e propriedades elásticas arteriais"

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