Cholesterol 7α-hydroxylase (CYP7A1) activity is modified after chronic ingestion of depleted uranium in the rat

Racine, Radjini; Grandcolas, Line; Grison, Stéphane; Stéfani, Johanna; Delissen, Olivia; Gourmelon, P.; Veyssière, G.; Souidi, Maâmar

doi:10.1016/j.jsbmb.2010.03.066

Cited by 16 publications

(15 citation statements)

References 42 publications

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“…Thus, DU contamination had no effect on general health parameters and cholesterol homeostasis at body level in apo E-deficient mice contaminated for 3 months. These results are in accordance with a previous study performed on healthy rodents [2].…”

Section: Parameterssupporting

confidence: 94%

“…Among systems affected after exposure to DU, we can note the cholesterol metabolism. A previous study has effectively shown that cholesterol metabolism was modulated at molecular level in the liver of rats drinking DUsupplemented water (40 mg L −1 ) for 9 months [2]. However, the contaminated animals did not display any pathological feature at organ or body level.…”

Section: Introductionmentioning

confidence: 89%

“…Total and free cholesterol in samples of liver were assayed using the Amplex Red Cholesterol Assay kit (Invitrogen-Life technologies, Cergy-Pontoise, France) following the manufacturer's instructions and in accordance with our previous paper [2]. The dilutions of samples were 1/12.…”

Section: Cholesterol Assay In the Livermentioning

confidence: 99%

“…The oligonucleotide sequences of primers were previously published [2]. The plates were analyzed on an AbiPrism 7900 Sequence Detection System (Applied Biosystems, Courtaboeuf, France) in accordance with previous papers [2,6]. Results were normalized to hypoxanthine-guanine phosphoribosyltransferase (HPRT) and fold-inductions calculated relatively to the control group.…”

Section: Real-time Pcrmentioning

confidence: 99%

“…Reaction intensity was determined by computer-assisted densitometry (Fuji LAS 3000, Raytest, France). The protein expression assay method used in this paper is in accordance with the previous paper [2].…”

Section: Assay Of Protein Levelsmentioning

confidence: 99%

See 4 more Smart Citations

Influence of depleted uranium on hepatic cholesterol metabolism in apolipoprotein e-deficient mice

Souidi

Racine

Grandcolas

et al. 2012

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Parameterssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 89%

Section: Cholesterol Assay In the Livermentioning

confidence: 99%

Section: Real-time Pcrmentioning

confidence: 99%

Section: Assay Of Protein Levelsmentioning

confidence: 99%

See 3 more Smart Citations

Influence of depleted uranium on hepatic cholesterol metabolism in apolipoprotein e-deficient mice

Souidi

Racine

Grandcolas

et al. 2012

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

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Berberrubine and its analog, hydroxypropyl‐berberrubine, regulate LDLR and PCSK9 expression via the ERK signal pathway to exert cholesterol‐lowering effects in human hepatoma HepG2 cells

Cao

Yan

et al. 2018

J of Cellular Biochemistry

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Berberine (BBR), the major isoquinoline alkaloid in Chinese herb Rhizoma coptidis, has significant lipid‐lowering effect by upregulating hepatic low‐density lipoprotein receptor (LDLR) expression. In a previous study, we have indicated that berberrubine (M3), a major metabolite of BBR in vivo, displays the most potential hypolipidemic effects via upregulating LDLR expression in human hepatoma (HepG2) cells compared with BBR and 3 other metabolites. Accordingly, 9 M3 analogs (A1‐A9) were modified at the C9 position. We aimed to find a new promising agent by evaluating the cholesterol‐lowering effect and clarifying the related molecular mechanism. In the current study, the cellular cholesterol content was assayed with a commercial cholesterol assay kit. Real‐time polymerase chain reaction and Western blot assay were used to explore the molecular mechanism of M3 and its analogs on the hypolipidemic effect. Among M3 and its analogs, hydroxypropyl‐berberrubine (A8) exhibited the highest potential effects on the upregulation of LDLR expression, which was accompanied by a steady decline of proprotein convertase subtilisin/kexin type 9 (PCSK9) messenger RNA and protein levels. Furthermore, inhibition of extracellular signal‐regulated kinase (ERK) activity with PD98059 prevented the upregulation of LDLR and downregulation of PCSK9 induced by A8. The current study revealed that M3 and its structurally modified analog, A8, could regulate hepatic LDLR and PCSK9 expression to exert lipid‐lowering effects via the ERK signal pathway, while A8 showed a stronger effect and might be a promising drug candidate against hyperlipidemia.

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Metabolomics identifies a biological response to chronic low-dose natural uranium contamination in urine samples

et al. 2013

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Because uranium is a natural element present in the earth’s crust, the population may be chronically exposed to low doses of it through drinking water. Additionally, the military and civil uses of uranium can also lead to environmental dispersion that can result in high or low doses of acute or chronic exposure. Recent experimental data suggest this might lead to relatively innocuous biological reactions. The aim of this study was to assess the biological changes in rats caused by ingestion of natural uranium in drinking water with a mean daily intake of 2.7 mg/kg for 9 months and to identify potential biomarkers related to such a contamination. Subsequently, we observed no pathology and standard clinical tests were unable to distinguish between treated and untreated animals. Conversely, LC–MS metabolomics identified urine as an appropriate biofluid for discriminating the experimental groups. Of the 1,376 features detected in urine, the most discriminant were metabolites involved in tryptophan, nicotinate, and nicotinamide metabolic pathways. In particular, N-methylnicotinamide, which was found at a level seven times higher in untreated than in contaminated rats, had the greatest discriminating power. These novel results establish a proof of principle for using metabolomics to address chronic low-dose uranium contamination. They open interesting perspectives for understanding the underlying biological mechanisms and designing a diagnostic test of exposure.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-013-0544-7) contains supplementary material, which is available to authorized users.

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Cholesterol 7α-hydroxylase (CYP7A1) activity is modified after chronic ingestion of depleted uranium in the rat

Cited by 16 publications

References 42 publications

Influence of depleted uranium on hepatic cholesterol metabolism in apolipoprotein e-deficient mice

Influence of depleted uranium on hepatic cholesterol metabolism in apolipoprotein e-deficient mice

Berberrubine and its analog, hydroxypropyl‐berberrubine, regulate LDLR and PCSK9 expression via the ERK signal pathway to exert cholesterol‐lowering effects in human hepatoma HepG2 cells

Metabolomics identifies a biological response to chronic low-dose natural uranium contamination in urine samples

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