Cholera Toxin β Subunit Induces the Differentiation of Human Medulloblastoma Cell Line DEV in a Neuronal Pathway

Dufay, Natalie; Belin, Marie‐Françoise; Confavreux, Christian; Touraine-Moulin, Françoise; Derrington, Edmund

doi:10.1111/j.1460-9568.1994.tb00554.x

Cited by 10 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This has fuelled speculation that PNET may derive from the transformation of multipotent neural precursors (Tohyama et al, 1992;Trojanowski et al, 1994;Valtz et al, 1991;Rorke et al, 1997). The Dev cell line is unique among PNET cell lines because Dev cells may express either neuronal or glial phenotypes when cells are cultivated in medium containing FCS (Dufay et al, 1994). Markers of mature neural cells, however, are not found in Dev cells when they are cultivated in the absence of serum.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies we have established that Dev cells express neuronal and glial antigens when cultured in the medium containing high levels of fetal calf serum (Dufay et al, 1994;Giraudon et al, 1993). The expression of mature cell type-speci®c antigens such as neuro®laments, GFAP or galactocerebroside is not observed when Dev cells are plated and maintained in serum free medium (EA Derrington, C Bellin, N Dufay, A Jouvet, S Bertrand, G St-Pierre and M-F Belin, submitted).…”

Section: Dev Cells Express An Undi Erentiated Neuroepithelial Phenotymentioning

confidence: 99%

“…Previous investigations had suggested that Dev cells were capable of di erentiation towards either the astrocytic or neuronal pathways in response to exogenous stimuli (Dufay et al, 1994;Giraudon et al, 1993). The question of whether distinct subpopulations of cells within the cell line are responding was not addressed.…”

Section: Monoclonal Subclones Of the Dev Cell Linementioning

confidence: 99%

“…We have described a cell line, Dev, derived from a cerebellar PNET (medulloblastoma), which is unique among PNET cell lines because subpopulations of Dev cells can be induced to di erentiate towards neuronal or astrocytic lineages when they are cultivated in fetal calf serum (FCS) (Dufay et al, 1994;Giraudon et al, 1993). However, Dev cells are intrinsically undifferentiated in that they do not express mature neural markers when plated and maintained in the absence of FCS.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Human primitive neuroectodermal tumour cells behave as multipotent neural precursors in response to FGF2

Dufay

Rudkin

et al. 1998

Oncogene

View full text Add to dashboard Cite

Primitive neuroectodermal tumours (PNET) are thought to derive from the malignant transformation of pluripotent CNS precursors although this hypothesis has yet to be tested in vitro. Here we show that cells of a human PNET cell line`Dev' express functional ®broblast growth factor (FGF) receptors (FGFR) and respond to FGF2 as multipotent CNS precursors. FGF2 induces tyrosine phosphorylation of FGFR-1 and FGFR-2 and many cellular substrates including MAP kinases and stimulates proliferation. Cells detach from plastic substrates and proliferate to form large clusters of undi erentiated cells. After adhesion to polylysine, cells migrate out from the clusters and di erentiate. The majority of di erentiated cells express neuronal phenotypes but distinct subpopulations express oligodendrocytic and astrocytic markers. Mature neural di erentiation markers are not otherwise detected in Dev cells in de®ned medium. Identical results were obtained with 12 monoclonal subclones as well as the parent cell line, con®rming that Dev cells are multipotent. This extends evidence that multipotent CNS precursors are the cellular substrate from which certain PNET develop and shows that FGF2 is a potent proliferation and di erentiation inducer for PNET cells in vitro, suggesting that FGF2 may also modulate the evolution of PNET in vivo. Finally our results suggest that PNET cell lines may provide models to elucidate the biology of human multipotent CNS precursors.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Dev Cells Express An Undi Erentiated Neuroepithelial Phenotymentioning

confidence: 99%

Section: Monoclonal Subclones Of the Dev Cell Linementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human primitive neuroectodermal tumour cells behave as multipotent neural precursors in response to FGF2

Dufay

Rudkin

et al. 1998

Oncogene

View full text Add to dashboard Cite

show abstract

“…The cellular model for this initial research concerning neural stem cells was the cell line derived from human medulloblastoma -named DEV (Dufay et al, 1994). It was characterized by indefinite growth and ability to differentiate into three neuronal cell types, thus showing neural stem cell line character (Derrington et al, 1998).…”

Section: Historical Notementioning

confidence: 99%

A novel, neural potential of non-hematopoietic human umbilical cord blood stem cells

Domanska-Janik¹,

Bużañska²,

Łukomska³

2008

Int. J. Dev. Biol.

View full text Add to dashboard Cite

From the time of discovery that among the cord blood mononuclear cell population there are cells capable of changing their fate towards the neural lineage and producing functional neurons and macroglial cells, our attempts have been focused on the understanding of the underlying mechanism of this transition. We have deciphered the first steps of neural stem/ progenitor gene induction in aggregating culture of cord blood mononuclear cells, their rapid phenotypic conversion under the influence of neuromorphogenic signals due to mitogen activation and their ability to expand and develop a prototypic, long-living line with neural stem cell properties. Evidence has accumulated that human umbilical cord-derived and neurally committed cells, due to their capacity for self-renewal, multilineage differentiation, plasticity and ability for long-lasting growth in vitro, provide unique material for the cell therapy of a wide spectrum of neurological diseases. The putative regenerating potential of these cord blood-derived neural stem/progenitor cells was evaluated after transplantation in experimental models of brain injury. In spite of initial promising data, the results indicate an urgent need to improve available animal model protocols in order to increase immuno-tolerance toward transplanted human cells.

show abstract

Human medulloblastoma cell line DEV is a potent tool to screen for factors influencing differentiation of neural stem cells

Bużañska¹,

Spassky²,

Belin³

et al. 2001

J of Neuroscience Research

View full text Add to dashboard Cite

The aim of our study was to investigate whether a human neural cell line could be used as a reliable screening tool to examine the functional conservation, in humans, of transcription factors involved in neuronal or glial specification in other species. Gain-of-function experiments were performed on DEV cells, a cell line derived from a human medulloblastoma. Genes encoding nine different transcription factors were tested for their influence on the process of specification of human DEV cells towards a neuronal or glial fate. In a first series of experiments, DEV cells were transfected with murine genes encoding transcription factors known to be involved in the neuronal differentiation cascade. Neurogenins-1, -2, and -3; Mash-1; and NeuroD increased the differentiation of DEV cells towards a neuronal phenotype by a factor of 2-3.5. In a second series of experiments, we tested transcription factors involved in invertebrate glial specification. In the embryonic Drosophila CNS, the development of most glial cells depends on the master regulatory gene glial cell missing (gcm). Expression of gcm in DEV cells induced a twofold increase of astrocytic and a sixfold increase of oligodendroglial cell types. Interestingly, expression of tramtrack69, which is required in all Drosophila glial cells, resulted in a fourfold increase of only the oligodendrocyte phenotype. Expression of the related tramtrack88 protein, which is not expressed in the fly glia, or the C. elegans lin26 protein showed no effect. These results show that the Drosophila transcription factor genes tested can conserve their function upon transfection into the human DEV cells, qualifying this cell line as a screening tool to analyze the mechanisms of neuronal and glial specification. J. Neurosci. Res. 65:17-23, 2001.

show abstract

Cholera Toxin β Subunit Induces the Differentiation of Human Medulloblastoma Cell Line DEV in a Neuronal Pathway

Cited by 10 publications

References 37 publications

Human primitive neuroectodermal tumour cells behave as multipotent neural precursors in response to FGF2

Human primitive neuroectodermal tumour cells behave as multipotent neural precursors in response to FGF2

A novel, neural potential of non-hematopoietic human umbilical cord blood stem cells

Human medulloblastoma cell line DEV is a potent tool to screen for factors influencing differentiation of neural stem cells

Contact Info

Product

Resources

About