Cholera toxin and retinoic acid slow the growth rate of human keratinocytes in low-calcium, serum-free culture

Pluff, Danielle; Dunham, William; Marcelo, Cynthia L.; Kageyama, Hiromi

doi:10.1016/j.jdermsci.2003.10.005

Cited by 1 publication

(1 citation statement)

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“…Although there was a significant inhibition of proliferation for the groups of 8 keratinocyte cultures, this could not be demonstrated in cultures from all donors. In contrast to these results are those obtained in cultures of adult human keratinocytes cultivated in 'prodifferentiating', high Ca 2+ mediums and growth-factor-deficient medium in which all-trans RA in a concentration of as low as 1 M may inhibit the proliferation of keratinocytes [24,28] . This discrepancy might be at least partly explained by the fact that we used a complete keratinocyte growth medium supplemented with growth factors and low Ca 2+ to provide a milieu for optimal growth of primary keratinocytes.…”

Section: Discussioncontrasting

confidence: 58%

Antiproliferative Effect of Vitamin A and D Analogues on Adult Human Keratinocytes in vitro

Popadić

Ramić²,

Medenica³

et al. 2008

Skin Pharmacol Physiol

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Background: Vitamin A and D analogues play an important role in epidermal homeostasis and are used in the treatment of various skin diseases. The failure of retinoid and vitamin D treatments is sometimes difficult to explain. Methods: We analyzed the effect of all-trans retinoic acid (all-trans RA), 13-cis retinoic acid (13-cis RA), ergocalciferol and cholecalciferol in keratinocyte cultures established from adult donors, on the cell proliferation by means of [³H]thymidine incorporation and apoptosis after fluorescein diacetate/trypan blue staining. Results: All tested agents exerted a dose-dependent inhibition of keratinocyte proliferation in the concentration range of 1.25–5 µM. Based on IC₅₀ values, the antiproliferative efficiency was as follows: cholecalciferol > ergocalciferol = all-trans RA > 13-cis RA. The observed effect of cholecalciferol and ergocalciferol, but not retinoids, involved the induction of apoptotic cell death. Combining vitamins A and D did not further increase the proliferation block and even displayed an antagonistic effect. Conclusion: The susceptibility of keratinocytes to the antiproliferative action of vitamins A and D was markedly different in cell cultures derived from different donors, indicating a possible predictive value of the in vitro testing for the efficiency of the clinical response to these agents.

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Section: Discussioncontrasting

confidence: 58%