Cholecystokinin receptors in Atlantic salmon: molecular cloning, gene expression, and structural basis

Rathore, Raja Mansingh; Angotzi, Anna Rita; Jordal, Ann‐Elise Olderbakk; Rønnestad, Ivar

doi:10.1002/phy2.69

Cited by 6 publications

(4 citation statements)

References 43 publications

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“…The action of CCK is initiated by its binding to two subtypes of cognate receptors (CCK-1R and CCK-2R), which results in satiety ( 197 ). Cck receptor genes have been isolated in yellowtail ( cck-1r ) ( 217 ), Atlantic salmon ( cck-1r, cck-2r1 , and cck-2r2 ) ( 218 ), and goldfish ( cck-1r and cck-2r ) ( 219 ). The primary structure of fish Cck receptors as well as their tissue distribution patterns is highly conserved; cck-1r is widely distributed within the GI-tract, while cck-2r is mainly expressed in the brain.…”

Section: Peripheral Signalsmentioning

confidence: 99%

Appetite-Controlling Endocrine Systems in Teleosts

et al. 2017

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Mammalian studies have shaped our understanding of the endocrine control of appetite and body weight in vertebrates and provided the basic vertebrate model that involves central (brain) and peripheral signaling pathways as well as environmental cues. The hypothalamus has a crucial function in the control of food intake, but other parts of the brain are also involved. The description of a range of key neuropeptides and hormones as well as more details of their specific roles in appetite control continues to be in progress. Endocrine signals are based on hormones that can be divided into two groups: those that induce (orexigenic), and those that inhibit (anorexigenic) appetite and food consumption. Peripheral signals originate in the gastrointestinal tract, liver, adipose tissue, and other tissues and reach the hypothalamus through both endocrine and neuroendocrine actions. While many mammalian-like endocrine appetite-controlling networks and mechanisms have been described for some key model teleosts, mainly zebrafish and goldfish, very little knowledge exists on these systems in fishes as a group. Fishes represent over 30,000 species, and there is a large variability in their ecological niches and habitats as well as life history adaptations, transitions between life stages and feeding behaviors. In the context of food intake and appetite control, common adaptations to extended periods of starvation or periods of abundant food availability are of particular interest. This review summarizes the recent findings on endocrine appetite-controlling systems in fish, highlights their impact on growth and survival, and discusses the perspectives in this research field to shed light on the intriguing adaptations that exist in fish and their underlying mechanisms.

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Section: Peripheral Signalsmentioning

confidence: 99%

Appetite-Controlling Endocrine Systems in Teleosts

et al. 2017

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“…CCKBR is also found in a few types of cells in gastric mucosa, pancreas, and gastrointestinal tract and its functions in mammals are involved in gut development and enzyme secretion rather than gut motility (Guilloteau et al, 2006). In fish, the tissue distribution of the CCK receptors have been investigated in a few species such as Atlantic salmon ( Salmon salar ) (Rathore et al, 2013), yellowtail Seriola quinqueradiata (Furutani et al, 2013), and goldfish (Tinoco et al, 2015). However, a full understanding of the CCK receptors role in gastrointestinal functions is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Effects of Cholecystokinin (CCK) on Gut Motility in the Stomachless Fish Ballan Wrasse (Labrus bergylta)

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Giroud-Argoud

et al. 2019

Front. Neurosci.

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Cholecystokinin (CCK) is well-known as a key hormone that inhibits stomach emptying and stimulates midgut motility in gastric species. However, the function of CCK related to gut motility in agastric fish, especially in fish with a short digestive tract such as ballan wrasse, remains unknown. Here we present a detailed description of the spatio-temporal quantification of intestinal motility activity in vitro comprising the complete intestinal tract in ballan wrasse. We show that CCK modulates intestinal motility, having multiple effects on motility patterns depending on location in the gut and types of contractions. CCK reduced propagating contractions in the foregut, but it increased both non-propagating and propagating contractions in the hindgut. CCK also altered the direction of propagating contractions, as it reduced anterograde ripples and slow propagating contractions. The velocity of propagating contractions was slowed down by CCK. CCK also reduced the amplitude of standing contractions and ripples, but it did not alter the amplitude of slow propagating contractions. The presence of CCKA receptor antagonist modulated the motility responses of ballan wrasse intestines when exposed to CCK. We also showed that CCK reduced the intestinal length and stimulated motility to empty the gallbladder. Based on our findings we hypothesize that CCK, mainly through the CCKA receptor, modulates non-propagating and propagating contractions to optimize digestion and absorption and regulate the intestinal evacuation in ballan wrasse. We also found evidence that the modulation of intestinal motility by CCK is different in agastric fish from that in gastric vertebrates. We suggest that this is an evolutionary adaptation to optimize digestion without a stomach.

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“…Cholecystokinin (CCK), a key hormone in the control of digestion, is released when food is present in the gut in both mammals [52][53][54] and fish [55][56][57]. CCK performs its functions related to gastrointestinal motility and digestion mainly through Cholecystokinin A Receptor (CCKAR) in mammals (reviewed by Staljanssens et al [58]) and in fish [59][60][61]. We have previously found that in ballan wrasse, CCK works mainly through CCKA receptors to suppress propulsive contractions, thus prolonging the residence of food in the bulbous for optimal digestion [17].…”

Section: Plos Onementioning

confidence: 99%

Physical and nutrient stimuli differentially modulate gut motility patterns, gut transit rate, and transcriptome in an agastric fish, the ballan wrasse

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Etayo

et al. 2021

PLoS ONE

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The effects of nutrient and mechanical sensing on gut motility and intestinal metabolism in lower vertebrates remains largely unknown. Here we present the transcriptome response to luminal stimulation by nutrients and an inert bolus on nutrient response pathways and also the response on gut motility in a stomachless fish with a short digestive tract; the ballan wrasse (Labrus berggylta). Using an in vitro model, we differentiate how signals initiated by physical stretch (cellulose and plastic beads) and nutrients (lipid and protein) modulate the gut evacuation rate, motility patterns and the transcriptome. Intestinal stretch generated by inert cellulose initiated a faster evacuation of digesta out of the anterior intestine compared to digestible protein and lipid. Stretch on the intestine upregulated genes associated with increased muscle activity, whereas nutrients stimulated increased expression of several neuropeptides and receptors which are directly involved in gut motility regulation. Although administration of protein and lipid resulted in similar bulbous evacuation times, differences in intestinal motility, transit between the segments and gene expression between the two were observed. Lipid induced increased frequency of ripples and standing contraction in the middle section of the intestine compared to the protein group. We suggest that this difference in motility was modulated by factors [prepronociceptin (pnoca), prodynorphin (pdyn) and neuromedin U (nmu), opioid neurotransmitters and peptides] that are known to inhibit gastrointestinal motility and were upregulated by protein and not lipid. Our findings show that physical pressure in the intestine initiate contractions propelling the bolus distally, directly towards the exit, whereas the stimuli from nutrients modulates the motility to prolong the residence time of digesta in the digestive tract for optimal digestion.

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Cholecystokinin receptors in Atlantic salmon: molecular cloning, gene expression, and structural basis

Cited by 6 publications

References 43 publications

Appetite-Controlling Endocrine Systems in Teleosts

Appetite-Controlling Endocrine Systems in Teleosts

Effects of Cholecystokinin (CCK) on Gut Motility in the Stomachless Fish Ballan Wrasse (Labrus bergylta)

Physical and nutrient stimuli differentially modulate gut motility patterns, gut transit rate, and transcriptome in an agastric fish, the ballan wrasse

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