Choice of material for above-knee femoropopliteal bypass graft

Michaels, J

doi:10.1002/bjs.1800760105

Cited by 123 publications

(45 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In case of small-diameter bypasses, ePTFE is the material most frequently used. Both clinical as well as basic studies have shown that ePTFE performs inferior to veins when used as bypass grafts [1, 2]. This led to the concept of cell seeding in which autologous endothelial cells are transplanted on ePTFE and so form an anticoagulant layer between blood and prosthesis [29, 30, 31].…”

Section: Discussionmentioning

confidence: 99%

“…In particular, small-diameter prosthetic vascular grafts perform poorly when compared to normal arteries or autologous vein grafts [1], resulting in a high occlusion rate, especially during the 1st year [2]. It has been generally accepted that this is a consequence of platelet adhesion and activation of the coagulation cascade on the graft [3, 4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Thrombomodulin Activity of Fat-Derived Microvascular Endothelial Cells Seeded on Expanded Polytetrafluorethylene

Joosten¹,

Verhagen²,

Heijnen-Snyder³

et al. 1999

J Vasc Res

View full text Add to dashboard Cite

Lining the luminal surface of prosthetic vascular grafts with endothelial cells (cell seeding) will lower its thrombogenicity. Commonly used macrovascular human adult endothelial cells (HAEC) require in vitro cultivation before large enough numbers are obtained to cover grafts confluently. Fat-derived microvascular endothelial cells (MVEC) prove to be a good alternative as they can be harvested in much larger numbers while showing similar antithrombotic and fibrinolytic characteristics. An important anticoagulant function of macrovascular endothelial cells is due to the activity of thrombomodulin (TM) on their surface. In this study, the presence and functional activity of TM on fat-derived microvascular cells used in cell seeding was investigated. The expression and localization of TM on MVEC was studied using immunohistochemistry. Functional activity of TM on MVEC was measured by the generation of activated protein C (APC) and was compared to human umbilical vein endothelial cells (HUVEC). TM activity was studied in MVEC seeded on expanded polytetrafluorethylene (ePTFE) vascular prostheses and compared to blank prostheses. We found that TM was expressed on the surface of MVEC, both in vivo and vitro. TM-dependent generation of APC differed significantly between MVEC and HUVEC (3.98 ± 1.2 vs. 3.0 ± 0.7 nM, respectively). After seeding MVEC on vascular prostheses, TM activity did not change. APC generation was significantly higher on MVEC-seeded vascular grafts compared to blank grafts (4.0 ± 0.7 vs. 1.7 ± 0.5 nM, respectively). We conclude that TM is present and highly active on cultured MVEC. When seeded on ePTFE, MVEC retain the possibility to inhibit thrombin coagulant activity and to activate protein C. Therefore, since MVEC are readily available, the anticoagulant properties demonstrated here indicate that this cell type is suitable for cell seeding of vascular prostheses.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thrombomodulin Activity of Fat-Derived Microvascular Endothelial Cells Seeded on Expanded Polytetrafluorethylene

Joosten¹,

Verhagen²,

Heijnen-Snyder³

et al. 1999

J Vasc Res

View full text Add to dashboard Cite

show abstract

“…The greater saphenous vein is a better substitute for femoropopliteal bypass than a prosthetic graft, 1,2 However, availability depends on the condition of the vein. Minor limited dilatation can be corrected by plication, tuck-stitching, or resection-anastomosis.…”

Section: Introductionmentioning

confidence: 99%

Prosthetic Reinforcement of Varicose Saphenous Vein Grafts for Infrainguinal Bypass

Soury

Peillon

Watelet

et al. 1999

Annals of Vascular Surgery

View full text Add to dashboard Cite

“…31 It is assumed that this is a consequence of platelet adhesion and activation. 32 A vascular graft that is lined with living cells capable of protecting it against unintentional activation of the hemostatic response would theoretically be less susceptible to thrombosis and occlusion.…”

Section: Discussionmentioning

confidence: 99%

Thrombogenicity and procoagulant activity of human mesothelial cells.

Pronk¹,

Groot²,

Papendrecht³

et al. 1992

Arterioscler Thromb

View full text Add to dashboard Cite

Cell seeding may decrease the thrombogenicity of implanted vascular grafts, but its application is hampered by the limited availability of autologous endothelial cells. We studied the interaction of alternate cells, human peritoneal mesothelial cells, with whole blood in a flow chamber. When titrated blood was perfused over mesothelial cells, platelet adhesion was seen on the intercellular matrix but not on the cells themselves. Pet-fusions with blood anticoagulated with low-molecular-weight heparin resulted in fibrin formation at the surface of mesothelial cells but not at the surface of human umbilical venous endothelial cells. At shear rates of 200 sec~' fibrin deposition on the mesothelial cell surface increased during the first 5 minutes to 5.7 ±1.06 fi% fibrin per square centimeter, whereafter these values stabilized. The procoagulant activity of cultured mesothelial cells was higher than that of peritoneal membrane studied ex vivo. However, cultured mesothelial cells incubated with polyclonal antibodies against tissue factor showed a significant decrease in procoagulant activity. We conclude that human peritoneal mesothelial cells may be used for cell seeding procedures, provided that their tissue factor expression can be controlled. 1 One of the causes for the inferior performance of prosthetic vascular grafts is thought to be their failure to establish a blood flowsupporting cellular surface.2 After implantation of a graft the lumen may be reduced by deposition of proteinaceous material and aggregated platelets. Consequently, the blood flow drops and may reach a critical level at which occlusion via thrombosis occurs. 3Previous workers have succeeded in seeding endothelial cells on vascular prostheses and have claimed reduced thrombogenicity of the implanted grafts. 4 -5 One of the major problems of endothelial cell seeding, however, is the limited availability of human endothelial cells. With current techniques, the luminal surface area of a donor vein that is needed to effectively seed a graft approximates half the surface area of that graft.67 In most cases it is then better to use the donor vein directly as a vascular conduit rather than as a source of endoFrom the Departments of Haematology (P.G. de G., J.J.S.) and Surgery (A.P., TJ.M.V. van V.), University Hospital Utrecht, and the Departments of Surgery (A.P., A.A.G.M.H. van P., P.L.) and Microbiology (H.A.V.),

show abstract

Choice of material for above-knee femoropopliteal bypass graft

Cited by 123 publications

References 43 publications

Thrombomodulin Activity of Fat-Derived Microvascular Endothelial Cells Seeded on Expanded Polytetrafluorethylene

Thrombomodulin Activity of Fat-Derived Microvascular Endothelial Cells Seeded on Expanded Polytetrafluorethylene

Prosthetic Reinforcement of Varicose Saphenous Vein Grafts for Infrainguinal Bypass

Thrombogenicity and procoagulant activity of human mesothelial cells.

Contact Info

Product

Resources

About