Chlortetracycline inhibits seed germination and seedling growth in Brassica campestris by disrupting H2O2 signaling

Cheong, Mi Sun; Yoon, Young-Eun; Kim, Jin Wook; Hong, Young Kyu; Kim, Sung Chul; Lee, Yong-Bok

doi:10.1186/s13765-019-0484-7

Cited by 15 publications

(23 citation statements)

References 29 publications

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“…Then, seed radicle emergence (i.e., rupturing of the seed coat), cotyledon opening (greening), and primary root growth were monitored. To measure the primary root length, photographs of captured B. campestris seedlings were measured using ImageJ ( ) (Bethesda, MD, USA) [ 80 ].…”

Section: Methodsmentioning

confidence: 99%

Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development

et al. 2020

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Veterinary antibiotics such as sulfonamides are widely used to increase feed efficiency and to protect against disease in livestock production. The sulfonamide antimicrobial mechanism involves the blocking of folate biosynthesis by inhibiting bacterial dihydropteroate synthase (DHPS) activity competitively. Interestingly, most treatment antibiotics can be released into the environment via manure and result in significant diffuse pollution in the environment. However, the physiological effects of sulfonamide during plant growth and development remain elusive because the plant response is dependent on folate biosynthesis and the concentration of antibiotics. Here, we present a chemical interaction docking model between Napa cabbage (Brassica campestris) DHPS and sulfamethoxazole and sulfamethazine, which are the most abundant sulfonamides detected in the environment. Furthermore, seedling growth inhibition was observed in lentil bean (Lens culinaris), rice (Oryza sativa), and Napa cabbage plants upon sulfonamide exposure. The results revealed that sulfonamide antibiotics target plant DHPS in a module similar to bacterial DHPS and affect early growth and the development of crop seedlings. Taking these results together, we suggest that sulfonamides act as pollutants in crop fields.

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Section: Methodsmentioning

confidence: 99%

Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development

et al. 2020

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“…Accumulated evidence indicates that veterinary drugs affect plant growth and development. We previously demonstrated that the treatment of B. campestris with Ery and CTC during the seedling growth period results in reduced Chl content [28,29]. Not surprisingly, these antibiotics were accumulated in the shoots of B. campestris seedlings [28,29].…”

Section: Introductionmentioning

confidence: 92%

“…We previously demonstrated that the treatment of B. campestris with Ery and CTC during the seedling growth period results in reduced Chl content [28,29]. Not surprisingly, these antibiotics were accumulated in the shoots of B. campestris seedlings [28,29]. Additionally, CTC accumulation in the leaves was greater during longer cultivation periods and in plants exposed to higher concentrations of antibiotics [29].…”

Section: Introductionmentioning

confidence: 94%

“…Not surprisingly, these antibiotics were accumulated in the shoots of B. campestris seedlings [28,29]. Additionally, CTC accumulation in the leaves was greater during longer cultivation periods and in plants exposed to higher concentrations of antibiotics [29]. Physiologically, CTC blocks RBOH-mediated oxidative signaling in B. campestris and inhibits primary root growth [29], while Ery influences the accumulation of photosynthetic proteins and inhibits plant development [28].…”

Section: Introductionmentioning

confidence: 99%

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Different Inhibitory Effects of Erythromycin and Chlortetracycline on Early Growth of Brassica campestris Seedlings

et al. 2021

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Veterinary antibiotics, including erythromycin (Ery) and chlortetracycline (CTC), are often detected in agricultural land. Although these contaminants affect plant growth and development, their effects on crops remain elusive. In this study, the effects of Ery and CTC on plant growth were investigated and compared by analyzing transcript abundance in Brassica campestris seedlings. Treatment with Ery and/or CTC reduced chlorophyll content in leaves and photosynthetic efficiency. Examination of the chloroplast ultrastructure revealed the presence of abnormally shaped plastids in response to Ery and CTC treatments. The antibiotics produced similar phenotypes of lower accumulation of photosynthetic genes, including RBCL and LHCB1.1. Analysis of the transcript levels revealed that Ery and CTC differentially down-regulated genes involved in the tetrapyrrole biosynthetic pathway and primary root growth. In the presence of Ery and CTC, chloroplasts were undeveloped and photosynthesis efficiency was reduced. These results suggest that both Ery and CTC individually affect gene expression and influence plant physiological activity, independently of one another.

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“…Actin was used as a housekeeping gene [24]. Gene expression was calculated using the 2 − Ct method [25].…”

Section: Quantitative Rt-pcr (Qrt-pcr)mentioning

confidence: 99%

Effect of sodium silicate on early growth stages of wheat under drought stress

Kim

Lee

et al. 2020

Appl Biol Chem

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Wheat yield is decreasing due to climate change, and a method to prevent decreasing yield during drought stress is desirable. In this study, wheat cultivars (Koso and Jokyung) were treated with 15% polyethylene glycol-6000 (PEG) and PEG + Si solution (6.5, 8.7, 13.1 and 26.1 mM). The effect of Si treatment on the alleviation of drought stress was measured using the germination test, shoot relative water content (RWC), seedling stage observation, and quantitative real time polymerase chain reaction (qRT-PCR). The results of root/shoot length ratio and shoot length ratio showed that Si treatment induced the alleviation of drought stress in Jokyung cultivar. The result of qRT-PCR showed the alleviation of drought stress in Koso cultivar. In addition, the results with shoot RWC and seedling stage observation showed that the alleviation effects of Si treatment was observed with both Koso and Jokyung cultivar at the high concentration of Si (26.1 mM). All these results suggest that Si treatment at a high concentration could be employed to alleviate drought stress in wheat.

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Chlortetracycline inhibits seed germination and seedling growth in Brassica campestris by disrupting H2O2 signaling

Cited by 15 publications

References 29 publications

Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development

Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development

Different Inhibitory Effects of Erythromycin and Chlortetracycline on Early Growth of Brassica campestris Seedlings

Effect of sodium silicate on early growth stages of wheat under drought stress

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