Chlorpyrifos induced oxidative stress to promote apoptosis and autophagy through the regulation of miR-19a-AMPK axis in common carp

Zhang, Qiaojian; Zheng, Shufang; Wang, Shengchen; Wang, Wei; Xing, Houjuan; Xu, Shiwen

doi:10.1016/j.fsi.2019.07.022

Cited by 84 publications

(28 citation statements)

References 57 publications

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“…Of particular relevance in this regard would be genes that encode proteins affecting AD etiology, such as that encoding Paraoxonase-1 (PON1) [136,137]. Pesticides are also known to affect the expression of micro(mi)RNAs [138][139][140]. In particular, Paraquat's modulate miRNAs affecting components of Wnt signaling [138] would be of direct relevance to AD [110,113].…”

Section: Epigenetic Mechanismsmentioning

confidence: 99%

Neuropathological Mechanisms Associated with Pesticides in Alzheimer’s Disease

Tang

2020

Toxics

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Environmental toxicants have been implicated in neurodegenerative diseases, and pesticide exposure is a suspected environmental risk factor for Alzheimer’s disease (AD). Several epidemiological analyses have affirmed a link between pesticides and incidence of sporadic AD. Meanwhile, in vitro and animal models of AD have shed light on potential neuropathological mechanisms. In this paper, a perspective on neuropathological mechanisms underlying pesticides’ induction of AD is provided. Proposed mechanisms range from generic oxidative stress induction in neurons to more AD-specific processes involving amyloid-beta (Aβ) and hyperphosphorylated tau (p-tau). Mechanisms that are more speculative or indirect in nature, including somatic mutation, epigenetic modulation, impairment of adult neurogenesis, and microbiota dysbiosis, are also discussed. Chronic toxicity mechanisms of environmental pesticide exposure crosstalks in complex ways and could potentially be mutually enhancing, thus making the deciphering of simplistic causal relationships difficult.

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Section: Epigenetic Mechanismsmentioning

confidence: 99%

Neuropathological Mechanisms Associated with Pesticides in Alzheimer’s Disease

Tang

2020

Toxics

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“…In zebrafish, CPF exposure has resulted in decreased hatching rate and increased apoptosis rate . Studies in common carp ( Cyprinus carpio ) have indicated that chlorpyrifos can induce oxidative stress and apoptosis through regulating the miR‐19a‐AMPK pathway in the kidney and can suppress neutrophil extracellular traps by inhibiting respiratory burst and increasing necroptosis which is caused by the PKC/MAPK Pathway . CPF induction on Xenopus laevis embryos has shown to reduce fgf8 , bmp4 , and sox9 genes expression which are master regulators of several early developmental pathways of the anatomical structures …”

Section: Introductionmentioning

confidence: 99%

DNA methylation changes of apoptotic genes in organogenesis stage of mice embryos by maternal chlorpyrifos induction

Barzi

Eftekhari

Doroud

et al. 2020

Environmental Toxicology

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The continued use of pesticides is one of the requirements of modern agriculture.Investigations have shown that pesticides can alter gene methylation and expression and subsequently may lead to abortion or birth of embryos with teratogenic disorders. In present study, 30 female NMRI mouse were divided in three experimental groups which in the CPF group, intraperitoneal chlorpyrifos was injected, in the sham group, DMSO was injected, and the control group without injection. The mice were mated and utinized 10 days' post gestation. The number of embryos in each fertilized female, maternal weight, and liver fibrosis was evaluated. The apoptosis pathway genes (caspase3, caspase9) and protein expressions (pro-caspase3, caspase3) of the embryos were evaluated with qRT-PCR and western blot, respectively. The DNA methylation of caspase3 and caspase9 were also assessed. The number of embryos and obtained maternal weight from the CPF group was significantly lower than other two groups. The mRNA expression of Caspase3 and Caspase9 were significantly higher in the CPF group. The protein expression evaluation confirmed the results achieved at the mRNA level. The percentage of Caspase9 DNA methylation in embryos collected from the CPF group was higher compared to the others. It can be considered that consumption of chlorpyrifos toxin can alter the DNA methylation and increase the expression of apoptotic genes. Therefore, continuous use of chlopyrifos may affect pregnancy by increasing the apoptosis pathway in the developing embryos which may lead to abortion or teratogenic disorders in newborn infants. K E Y W O R D Sapoptotic genes, chlorpyrifos, DNA methylation, embryo, organogenesis stage

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“…A previous study showed that AMPK regulation could affect apoptosis in CMs and a hyperglycemic zebrafish model 36 . Thus, to elucidate the possible mechanism of DCM, the expression levels of components of the AMPK pathway were examined in our models.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have shown that AMPK, a sensor of cellular energy status, is involved in cell apoptosis, and its function in cell apoptosis is often controversial 36 . AMPK is considered as a key regulator of cellular and systemic energy homeostasis, and is involved in various metabolic functions in diseases.…”

Section: Discussionmentioning

confidence: 99%

Effect of adenosine monophosphate‐activated protein kinase–p53–Krüppel‐like factor 2a pathway in hyperglycemia‐induced cardiac remodeling in adult zebrafish

Wang

Luo

et al. 2020

J of Diabetes Invest

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Aims/Introduction Diabetic cardiomyopathy is a type of myocardial disease. It causes left ventricular hypertrophy, followed by diastolic and systolic dysfunction, eventually leading to congestive heart failure. However, the underlying mechanism still requires further elucidation. Materials and Methods A high‐glucose zebrafish model was constructed by administering streptozocin intraperitoneally to enhance the development of cardiomyopathy and then treated with adenosine monophosphate‐activated protein kinase (AMPK) activator. Cardiac structure and function, and protein and gene expression were then analyzed. Cardiomyocytes (CMs) culture in vitro using lentivirus were used for detection of AMPK, p53 and Krüppel‐like factor 2a (klf2a) gene expression. Results In the hyperglycemia group, electrocardiogram findings showed arrhythmia, echocardiography results showed heart enlargement and dysfunction, and many differences, such as increased apoptosis and myocardial fiber loss, were observed. The phospho‐AMPK and klf2a expression were downregulated, and p53 expression was upregulated. Activation of phospho‐AMPK reduced p53 and increased klf2a expression, alleviated apoptosis in CMs and improved cardiac function in the hyperglycemic zebrafish. In vitro knockdown system of AMPK, p53 and klf2a using lentivirus illustrated an increased p53 expression and decreased klf2a expression in CMs by inhibiting AMPK. Repression of p53 and upregulation of klf2a expression were observed, but no changes in the expression of AMPK and its phosphorylated type. Conclusions In the model of streptozocin‐induced hyperglycemia zebrafish, the reduction of phosphorylated AMPK increased p53, which led to KLF2a decrease to facilitate apoptosis of CMs, inducing the cardiac remodeling and cardiac dysfunction. These results can be reversed by AMPK activator, which means the AMPK–p53–klf2a pathway might be a potential target for diabetic cardiomyopathy intervention.

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Chlorpyrifos induced oxidative stress to promote apoptosis and autophagy through the regulation of miR-19a-AMPK axis in common carp

Cited by 84 publications

References 57 publications

Neuropathological Mechanisms Associated with Pesticides in Alzheimer’s Disease

Neuropathological Mechanisms Associated with Pesticides in Alzheimer’s Disease

DNA methylation changes of apoptotic genes in organogenesis stage of mice embryos by maternal chlorpyrifos induction

Effect of adenosine monophosphate‐activated protein kinase–p53–Krüppel‐like factor 2a pathway in hyperglycemia‐induced cardiac remodeling in adult zebrafish

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