Chlorpyrifos-induced cell proliferation in human breast cancer cell lines differentially mediated by estrogen and aryl hydrocarbon receptors and KIAA1363 enzyme after 24 h and 14 days exposure

Moyano, Paula; García, José M.; Pelayo, Adela; Muñoz-Calero, Pilar; Frejo, María Teresa; Anadón, M.J.; Lobo, Margarita; Pino, Javier del

doi:10.1016/j.chemosphere.2020.126426

Cited by 31 publications

(31 citation statements)

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“…Recent studies have demonstrated that after a single and repeated treatment of chlorpyrifos and chlorpyrifos‐oxon, NCEH1 is elevated in MCF‐7 and MDA‐MA‐231 cells and induces proliferation. The results indicated that NCEH1 may participate with chlorpyrifos as factors in the induction of breast cancer 26 . Chang et al reported that NCEH1 was highly expressed in prostate cancer cells.…”

Section: Discussionmentioning

confidence: 87%

NAD(P)‐dependent steroid dehydrogenase‐like protein and neutral cholesterol ester hydrolase 1 serve as novel markers for early detection of gastric cancer identified using quantitative proteomics

Xiao

Xie

Liu

et al. 2020

Clinical Laboratory Analysis

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Background Gastric cancer (GC) is the third most common cause of cancer deaths worldwide. In the present study, we aimed to identify novel GC biomarkers by integrating isobaric tags of relative and absolute quantitation (iTRAQ) for aberrantly expressed proteins in GC patients. Methods Using stable isotope tags, we labeled an initial discovery group comprising four paired gastric cancer and adjacent gastric tissue samples, and subjected them to LC‐ESI‐MS/MS. We used a validation set comprising 129 paired gastric cancer and adjacent gastric tissues from patients and benign healthy controls to validate the candidate targets. Results We identified two proteins, NAD(P)‐dependent steroid dehydrogenase‐like (NSDHL) and neutral cholesterol ester hydrolase 1 (NCEH1), that were significantly overexpressed in GC tissues. The sensitivity and specificity of NSDHL were 80.6% and 74.4%, respectively, in GC compared with a sensitivity of 25.6% in adjacent tissues and 24% in benign healthy controls. The area under the ROC curve (AUC) for NSDHL was 0.810 for GC detection. Overexpression of NSDHL in GC was significantly correlated with local tumor invasion. The sensitivity and specificity of NCEH1 were 77.5% and 73.6%, respectively, in GC compared with a sensitivity of 26.4% in adjacent tissues and 20% in benign controls. The AUC for NSDHL was 0.792. Overexpression of NCEH1 was significantly associated with tumor histological classification and local invasion. Moreover, a combined analysis of NSDHL and NCEH1 achieved a sensitivity and specificity of 85.7% and 83%, respectively, and the AUC was 0.872. The combined analysis of NSDHL and NCEH1 was significantly correlated with histological grade and TNM Ⅱ‐Ⅳ staging. Conclusions iTRAQ‐labeled quantitative proteomics represents a powerful method to identify novel cancer biomarkers. The present study identified NSDHL and NCEH1 as useful biomarkers for screening, diagnosis, and prognosis of patients with gastric cancer.

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Section: Discussionmentioning

confidence: 87%

NAD(P)‐dependent steroid dehydrogenase‐like protein and neutral cholesterol ester hydrolase 1 serve as novel markers for early detection of gastric cancer identified using quantitative proteomics

Xiao

Xie

Liu

et al. 2020

Clinical Laboratory Analysis

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“…However, another concluded that malathion did not display any estrogenic activity [ 43 ]. Three studies demonstrated the estrogenic potential of chlorpyrifos by binding to sex hormones [ 44 ] and acting as estrogen agonists [ 45 , 46 ]. However, two other studies did not find any chlorpyrifos-induced endocrine disrupting potential [ 47 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, two other studies did not find any chlorpyrifos-induced endocrine disrupting potential [ 47 , 48 ]. Chlorpyrifos was also found to alter changes in gene expression [ 46 ], induce cell cycle arrest in the S and G2/M-phase vent [ 49 ], decrease cell proliferation [ 50 ], and increase oxidative stress [ 51 ]. A detailed summary of the main findings and their proposed cellular mechanisms are shown in Table 3 .…”

Section: Resultsmentioning

confidence: 99%

“…Among the in vitro studies, one reported that malathion and dimethoate did not possess any endocrine disrupting activity while another reported a mild endocrine disrupting activity through activation of the ERa pathway in malathion-treated breast cancer cells [ 42 , 43 ]. Chlorpyrifos was also shown to act as a potential Era and AhR agonist among two studies thereby increasing cell proliferation and viability that may eventually lead to breast tumorigenesis [ 46 , 49 ]. However, chlorpyrifos did not induce cell proliferation or exhibit ER activation in two other studies [ 47 , 48 ] and was found to decrease cell proliferation through mechanisms that may or may not involve the ER pathway [ 45 , 50 , 51 ], demonstrating the potential cytotoxic effects chlorpyrifos has on breast cell lines through endocrine disrupting mechanisms.…”

Section: Discussionmentioning

confidence: 99%

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Organophosphate Pesticide Exposure and Breast Cancer Risk: A Rapid Review of Human, Animal, and Cell-Based Studies

Yang

Lee

Park

2020

IJERPH

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Background: Organophosphate pesticides (OPs) are one of the most commonly used classes of insecticides in the U.S., and metabolites of OPs have been detected in the urine of >75% of the U.S. population. While studies have shown that OP exposure is associated with risk of neurological diseases and some cancers, the relationship between OP exposure and breast cancer risk is not well understood. Methods: The aim of this rapid review was to systematically evaluate published literature on the relationship between OP exposure and breast cancer risk, including both epidemiologic and laboratory studies. Twenty-seven full-text articles were reviewed by searching on Pubmed, EMBASE, and Cochrane databases. Results: Some human studies showed that malathion, terbufos, and chlorpyrifos were positively associated with human breast cancer risk, and some laboratory studies demonstrated that malathion and chlorpyrifos have estrogenic potential and other cancer-promoting properties. However, the human studies were limited in number, mostly included agricultural settings in several geographical areas in the U.S., and did not address cumulative exposure. Conclusions: Given the mixed results found in both human and laboratory studies, more research is needed to further examine the relationship between OP exposure and breast cancer risk, especially in humans in non-agricultural settings.

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“…The abuse of Chl could cause environmental pollution including water contamination, soil degradation, accumulation in crops and potential risk to animals [1 − 3]. Besides, exposure to Chl also has great threat to human health, such as affect male reproduction [4], neurotoxicity [5], nephrotoxicity [6], induce the proliferation of breast cancer cells [7,8] and acute toxicity [9]. Thus, the toxicity of Chl to environment and humans lead to urgently exploit sensitive, simple, low cost and on-site rapid methods for Chl analysis and detection, since the instrumental techniques (high performance liquid chromatography [10,11], gas chromatography [12], mass spectrometry [13], surface enhanced Raman scattering [14], etc.)…”

Section: Discussionmentioning

confidence: 99%

A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

Liu¹,

Yang³

et al. 2021

Preprint

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Background Chlorpyrifos (Chl) is an organophosphorus pesticide, which has toxicity to environment, animals and human beings. To overcome the shortages of traditional detection methods of small molecular, this study aimed to develop a sensitive, simple, low cost and on-site rapid method for Chl analysis and detection. Thus, we developed a simple label-free gold nanoparticles (AuNPs) based colorimetric biosensor aptasensor for Chl detection using an aptamer as the caputure probe. Results The Chl-aptamer with low dissociation constant (Kd) of 58.59 ± 6.08 (nM) was selected by ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for AuNPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately thus a self-aggregation of AuNPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the limit of detection as low as 14.46 nM. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, which showed satisfying results with excellent recovery values between 96.2% and 105.6% and acceptable RSD values below 5%. Conclusions The developed colorimetric aptasensor can serve as a promising candidate for Chl detection in the area of biosensors, which also showed a great potential in simple, cheap and rapid detection of Chl.

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Chlorpyrifos-induced cell proliferation in human breast cancer cell lines differentially mediated by estrogen and aryl hydrocarbon receptors and KIAA1363 enzyme after 24 h and 14 days exposure

Cited by 31 publications

References 69 publications

NAD(P)‐dependent steroid dehydrogenase‐like protein and neutral cholesterol ester hydrolase 1 serve as novel markers for early detection of gastric cancer identified using quantitative proteomics

NAD(P)‐dependent steroid dehydrogenase‐like protein and neutral cholesterol ester hydrolase 1 serve as novel markers for early detection of gastric cancer identified using quantitative proteomics

Organophosphate Pesticide Exposure and Breast Cancer Risk: A Rapid Review of Human, Animal, and Cell-Based Studies

A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

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